Serologic profile of serum antibody reactivity to SV40 mimotopes VP1 B (A) and VP2/3 C (B) and VPs B+C (C).

<p>Immunologic data are from serum samples from Multiple Sclerosis patients (MS), Healthy Subjects (HS1), Other Inflammatory Neurologic Diseases (OIND), Non-Inflammatory Neurologic Diseases (NIND) and Healthy subjects (HS2). Results are presented as values of optical density (OD) readings at λ 405 nm, of serum samples diluted at 1∶20, detected in indirect ELISA. In scatter dot plotting, each plot represents the dispersion of OD values to a mean level indicated by the line inside the scatter with Standard Error Mean (SEM) for each group of subjects analyzed. A) The mean OD of sera (VP B ± Std Error) in MS (0.32±0.02) were lower than that in HS1 (0.50±0.02) and in OIND (0.67±0.04). Moreover the mean OD of sera in OIND were higher than that in NIND (0.32±0.02) and HS2 (0.46±0.02). The mean OD of sera in NIND were lower than that in HS2. B) The mean OD of sera (VP C ± Std Error) in MS (0.26±0.03) were lower than that in HS1 (0.43±0.02). C) The mean OD of sera (VPs B+C ± Std Error) in MS (0.29±0.02) were lower than that in HS1 (0.46±0.02) and in OIND (0.49±0.03). Moreover, the mean OD of sera in OIND were higher than that in NIND (0.34±0.02). The mean OD in NIND were lower than that in HS2 (0.43±0.02) and in OIND sera. Statistical analysis was performed using Anova and Newman-Keuls Comparison test. (**<i>P</i><0.001; °<i>P</i><0.01).</p

Prevalence of immunoglobulin G antibodies reacting with Simian Virus 40 (SV40) viral protein (VP) mimotopes in serum samples of patients affected by MS, OIND, NIND and HS∧.

<p>∧Human sera were from patients affected by multiple sclerosis (MS), other inflammatory neurologic diseases (OIND), non-inflammatory neurologic diseases (NIND) and healthy subjects (HS1), (HS2).</p><p>*The prevalence of SV40 antibodies in MS patients is statistically significant lower that those detected in NIND patients (<i>P</i> = 0.0254) and in HS1 (<i>P</i> = 0.001), whereas no significant was the different prevalence detected in MS and OIND patients (<i>P</i>>0.05).</p><p>** The prevalence of SV40 antibodies in OIND patients is statistically significant lower that those detected in HS2 (<i>P</i> = 0.0403). The different prevalence of SV40 antibodies between the cohorts of NIND patients was not significant compared with the HS2 (<i>P</i>>0.05). Statistical analysis was performed using the χ² test.</p><p>Prevalence of immunoglobulin G antibodies reacting with Simian Virus 40 (SV40) viral protein (VP) mimotopes in serum samples of patients affected by MS, OIND, NIND and HS∧.</p