Factors influencing the Yield of Transgenic Mouse Embryos for Cryopreservation

Cryopreservation of pre-implantation embryos is  a powerful tool to save and handle the increasing  number of transgenic mouse lines, often consisting  of very small populations. However, the outcome  of embryos following superovulation and mating  varies and is one of the limiting factors for  cryopreservation. Using the data received together  with the cryopreservation of more than 100,000  mouse embryos, the role of embryo yield limiting  and of yield raising factors was analyzed: annual  rhythms, seasonal changes (possibly due to  environmental effects, such as extreme weather  conditions), infections of a mouse colony, or the  mating frequency of males. Then again to increase  embryo yields two pheromonal effects were  investigated: Especially the housing of female  donors in small groups (Lee-Boot effect), but also  the exposure to the future males’ bedding two  days before mating (Whitten effect) resulted in  remarkably higher embryo yields.

In vitro reconstitution of hnRNP particles

AbstractThe assembly of hnRNP-like particles was studied by in vitro reconstitution, UV-crosslinking and CsCl-equilibrium centrifugation. Using total nuclear protein and RNA extracts from HeLa cells for RNP reconstitution, RNP particles sedimenting with the same buoyant density of ϱ=1.4 g/cm3 as ‘native’ 40 S core hnRNPs were obtained. Under the stringent reconstitution conditions used, hnRNP complexes containing only the C1-core hnRNP protein could be identified

Dijagnostika stenoza intrakranijskih arterija primjenom transkranijskog bojom kodiranog ultrazvuka

Transcranial Doppler (TCD) scenography has been widely used as a diagnostic tool for intracranial hemodynamic alterations and in the last decade transcranial color-coded real-time sonography (TCCS) have been added to conventional TCD sonography in order to improve diagnostic yield. TCCS can reliably assess the 50% and <50% basal artery narrowing, and has proved useful for noninvasive management of patients with symptomatic intracranial stenoses.Transkranijski dopler (TCD) upotrebljava se kao dijagnostička metoda za intrakranijske hemodinamske poremećaje, a u zadnjem desetljeću uvođenje transkranijskog bojom kodiranog ultrazvuka (transcranial color-coded sonography - TCCS) doprinijelo je poboljšanju dijagnostike. Primjenom TCCS-a moguće je procijeniti suženje arterija na bazi mozga što se pokazalo kao vrijedna neinvazivna metoda kod stenoza intrakranijskih arterija

Functional rescue of the glomerulosclerosis phenotype in Mpv17 mice by transgenesis with the human Mpv17 homologue

The germ line insertion of a defective retrovirus into the Mpv17 gene of mice is associated with a recessive phenotype. Mice homozygous for the integration develop glomerulosclerosis at a young age. The phenotype resembles human glomerulosclerosis in its physiological parameters as well as in histology. A human homologue of the Mpv17 gene has been identified, isolated and analyzed. We here show that this gene, which has a role in the production of reactive oxygen species, can rescue the phenotype of Mpv17 deficient mice when introduced by transgenesis. This provides formal proof for the hypothesis that the phenotype is caused by the loss of function of the Mpv17 gene. It also provides evidence for the functional conservation of the Mpv17 gene in mammals and points to a potential role of this gene in human kidney disease

Dijagnostika stenoza intrakranijskih arterija primjenom transkranijskog bojom kodiranog ultrazvuka

Transcranial Doppler (TCD) scenography has been widely used as a diagnostic tool for intracranial hemodynamic alterations and in the last decade transcranial color-coded real-time sonography (TCCS) have been added to conventional TCD sonography in order to improve diagnostic yield. TCCS can reliably assess the 50% and <50% basal artery narrowing, and has proved useful for noninvasive management of patients with symptomatic intracranial stenoses.Transkranijski dopler (TCD) upotrebljava se kao dijagnostička metoda za intrakranijske hemodinamske poremećaje, a u zadnjem desetljeću uvođenje transkranijskog bojom kodiranog ultrazvuka (transcranial color-coded sonography - TCCS) doprinijelo je poboljšanju dijagnostike. Primjenom TCCS-a moguće je procijeniti suženje arterija na bazi mozga što se pokazalo kao vrijedna neinvazivna metoda kod stenoza intrakranijskih arterija

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Overexpression of Bcl-2 inhibits alveolar cell apoptosis during involution and accelerates c-myc-induced tumorigenesis of the mammary gland in transgenic mice

Expression of the apoptosis-inhibitory protein Bcl-2 has frequently been detected in human cancer including mammary carcinoma. The functional significance of its expression has been well established in experimental tumors of the lymphoid system, however, remains to be elucidated for epithelial tumors. In order to assess the role of Bcl-2 in mammary tumorigenesis we have generated WAP-bcl-2 transgenic mice. The strong overexpression of Bcl-2 in lactating mammary glands was preserved during early postlactational involution and apoptosis of alveolar epithelial cells was prevented without influencing the dedifferentiation of the milk-producing epithelium. Although Bcl-2 overexpression was not sufficient to induce spontaneous tumors it, however, led to an accelerated development of MMTV myc transgene-induced mammary tumors. In the mammary glands of MMTV myc transgenic mice, a high proportion of apoptotic cells was detected which was significantly reduced in the mammary glands of WAP-bcl-2/ MMTV myc double transgenic mice. Taken together, these results suggest that Bcl-2 contributes to mammary tumorigenesis by inhibiting apoptosis