Neutrophil migration in adults (n = 30) and in cord blood of healthy term neonates expressed as number of migrated cells on the ordinate and the tested chemoattractants (PBS-buffer and fMLP) on the abscissa.

<p>Neonates after vaginal delivery (n = 13), Cesarean section (CS, all, n = 31), CS under spinal anaesthesia (n = 13), CS under epidural anaesthesia (n = 11), CS under general anaesthesia (n = 7). Results are expressed as Mean ± SE. * p<0.05; ** p<0.01; *** p<0.001</p

Demoscopic data and results of adults and term neonates.

<p>Demoscopic data and results of adults and term neonates.</p

Neutrophil migration in cord blood of term and preterm neonates expressed as number of migrated cells on the ordinate and the tested chemoattractants (PBS-buffer and fMLP) and patient populations on the abscissa.

<p>Left: Term (n = 13) and preterm (n = 4) infants after vaginal delivery Right: Term (n = 11) and preterm (n = 18) infants after Cesarean section under epidural anaesthesia. Results are expressed as Mean ± SE. No statistically rsignificant differences.</p

L-Selectin expression on neutrophils granulocytes in adults (n = 30) and in cord blood of healthy term neonates expressed as relative fluorescence intensity (RFI) on the ordinate and the tested cell populations on the abscissa: non-migrated cells from PBS-insert (left) and migrated cells towards PBS-buffer (middle) and fMLP (right).

<p>Neonates after vaginal delivery (n = 13), Cesarean section (CS, all, n = 31), CS under spinal anaesthesia (n = 13), CS under epidural anaesthesia (n = 11), CS under general anaesthesia (n = 7). Results are expressed as Mean ± SE. * p<0.05; ** p<0.01; *** p<0.001</p

Shape change of PMN during migration through the membrane pores in a fluorescence microscopy analyse Diameter of the pores: 3 μm, see the bevelled, diagonal diameter.

<p>Shape change of PMN during migration through the membrane pores in a fluorescence microscopy analyse Diameter of the pores: 3 μm, see the bevelled, diagonal diameter.</p

Demoscopic data and results of the migration assay of the preterm neonates.

<p>Demoscopic data and results of the migration assay of the preterm neonates.</p

Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo-1

<p><b>Copyright information:</b></p><p>Taken from "Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo"</p><p>http://www.biomedcentral.com/1471-2172/8/31</p><p>BMC Immunology 2007;8():31-31.</p><p>Published online 28 Nov 2007</p><p>PMCID:PMC2217554.</p><p></p>chimeric mice (gray bar, n = 4)) and control mice (black bar, n = 6) before and during 15 min local administration of fMLP (1 μM). * indicates significant difference (p < 0.05) between chimeric and control mice

Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo-3

<p><b>Copyright information:</b></p><p>Taken from "Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo"</p><p>http://www.biomedcentral.com/1471-2172/8/31</p><p>BMC Immunology 2007;8():31-31.</p><p>Published online 28 Nov 2007</p><p>PMCID:PMC2217554.</p><p></p>o) addition of 1 mM Mnat 37°C for 30 min. Adherent (n = 7 mice) or control neutrophils (n = 5 mice) in percent of total cells added (A), microscopic images (B), increase of cell area (in μm, C) and frequency distribution of cell area (D) of adherent (n = 400 from 4 mice) and control neutrophils (n = 400 from 4 mice) upon stimulation for 30 min at 37°C. * indicates significant difference (p < 0.05), n.s., not significant

Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo-2

<p><b>Copyright information:</b></p><p>Taken from "Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo"</p><p>http://www.biomedcentral.com/1471-2172/8/31</p><p>BMC Immunology 2007;8():31-31.</p><p>Published online 28 Nov 2007</p><p>PMCID:PMC2217554.</p><p></p>uring gradual stimulation (A, right panel) leading to flattening out of the cell with a concomitant decrease in cell diameter perpendicular to the vessel wall (white arrows). Diameters of adherent leukocytes from chimeric mice (gray bar, n = 318 from 4 mice) and control mice (black bar, n = 419 from 6 mice) were measured perpendicular to the vessel wall before and during superfusion with fMLP (B). In addition, a cumulative frequency distribution of measured leukocyte diameters is given for (gray lines) and control leukocytes (black lines) after 1 min (dashed lines) and 15 min (solid lines) fMLP superfusion (C). * in (B): significant difference (p < 0.05) between chimeric and control mice. * in (C): significant difference (p < 0.05) in the distribution of control cell diameters at 15 min fMLP to all other groups

Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo-4

<p><b>Copyright information:</b></p><p>Taken from "Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo"</p><p>http://www.biomedcentral.com/1471-2172/8/31</p><p>BMC Immunology 2007;8():31-31.</p><p>Published online 28 Nov 2007</p><p>PMCID:PMC2217554.</p><p></p>ncy [(adherent cells/mmvessel surface area)/(systemic leukocyte count)] (B), and rolling flux fraction [%] (C) in chimeric mice (gray bar, n = 4) and control mice (black bar, n = 6). * indicates significant difference (p < 0.05) between chimeric and control mice