The purity of CD34⁺ separation decreases with an increasing number of MNC in CCB.

<p>MNC were isolated from CCB or FCB by washing with DNAse buffer or Ficoll-paque density gradient centrifugation as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046772#s2" target="_blank">materials and methods</a>. CD34⁺ stem cells were isolated from MNC by a positive magnetic separation of CD34⁺ cells and the purity was determined by flow cytometry. The correlations between the number of MNC and the purity of the CD34⁺ separation for CCB (A; no significance) and FCB (B) are shown. Correlations were evaluated using GraphPad Prism software and the Spearman rank correlation coefficient method.</p

Humanized mice generated functional human B and T cells.

<p>(A, B) The concentration of human IgM and IgG in the plasma of humanized mice was determined by cytometric bead array 19–21 weeks after intrahepatic transplantation of CD34⁺ cells from CCB or FCB. (C) Spleen cells were labeled with CFDA-SE and cultured for 5 d in the presence (dark gray bars) or absence (gray bars) of an anti-CD3 antibody. Proliferation of cells was determined by flow cytometry. Box plots depict median and 5–95% percentile. Level of significance is given.</p

Differences and parallelism in the purity of CD34⁺ -cell-purifications from FCB and blood bank CCB.

<p>(A) MNC were isolated from FCB and blood bank CCB by washing with DNAse buffer or Ficoll-paque density gradient centrifugation as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046772#s2" target="_blank">materials and methods</a>. CD34⁺ stem cells were isolated from MNC by a positive magnetic separation of CD34+ cells and the purity was analyzed by flow cytometry. Dot plots depict the percentage of CD34⁺ cells of one representative separation from CCB and FCB (lower panels). The quadrant was set according to the isotype controls (upper panels). (B) The bar chart depicts the percentage of CD34⁺ cells after isolation of CD34⁺ cells from 26 CCB and 37 FCB samples. Box plots depict median and 5–95% percentile. Level of significance is given. n. s. = no significance.</p

Differences and parallelism in the yield of CD34⁺ -cell-purifications from FCB and blood bank CCB.

<p>(A) MNC were isolated from CCB or FCB by washing with DNAse buffer or Ficoll-paque density gradient centrifugation as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046772#s2" target="_blank">materials and methods</a>. The total number of MNC per 10 ml cord blood is shown. (B) CD34⁺ cells were isolated from MNC by a positive magnetic separation of CD34⁺ cells. The number of CD34⁺ cells per 10 ml cord blood is shown.</p