Supplementary_Table – Supplemental material for The prognostic significance of tall cells in papillary thyroid carcinoma: A case-control study

<p>Supplemental material, Supplementary_Table for The prognostic significance of tall cells in papillary thyroid carcinoma: A case-control study by Sebastian Stenman, Päivi Siironen, Harri Mustonen, Johan Lundin, Caj Haglund and Johanna Arola in Tumor Biology</p

Clinical and laboratory data on the study subjects.

<p>Clinical and laboratory data on the study subjects.</p

Relative abundances (%) of the genus <i>Streptococcus</i>.

<p><b>a)</b> in controls and patients at different stages of PSC. <b>b)</b> in relation to ERC score. Lower and upper box hinges represent the 1^st and 3^rd quartiles, respectively. Whiskers represent 1.5 times the interquartile range. Bold lines represent medians and crosses the means.</p

Relative abundances (%) of bacteria in controls and patients at different stages of PSC.

<p><b>a)</b> Five most common phyla. <b>b)</b> Ten most common families. <b>c</b>) Ten most common genera.</p

Statistically significant results from differential abundance comparisons.

<p>Statistically significant results from differential abundance comparisons.</p

Shannon diversity.

<p><b>a)</b> in controls and patients at different stages of PSC. <b>b)</b> in controls compared to early stage patients at their 1^st ERC examination. Lower and upper box hinges represent the 1^st and 3^rd quartiles, respectively. Whiskers represent 1.5 times the interquartile range. Bold lines represent medians and crosses the means.</p

Immunohistochemical analysis of Ad5 and Ad5T122 infection in ex vivo tissue cultures of normal human liver and colorectal carcinoma liver metastasis.

<p>Precision-cut tumour (top and third row of panels) and liver (second row and bottom panels) tissue cultures were left uninfected (left panels) or infected with 10⁷ PFU (in 2 ml of media) of Ad5 (middle panels) or Ad5T122 (right panels), and fixed five days later for staining with haematoxylin-eosin (panels G-L) or an antibody against the viral E1A protein (panels A–F). Necrosis developing in the central area of the uninfected control tissue (panel J) is indicated with arrows.</p

Oncolytic potential of Ad5T122 and Ad5 in a lung cancer xenograft model.

<p>Mice were treated with 10⁷ PFU of Ad5, Ad5T122, or injected with a corresponding volume of PBS (Mock) on days 0, 2 and 4, as indicated by arrows. Tumour size was calculated as the volume of an ellipsoid. Tumour growth is expressed as a percentage increase from first day of virus injection. The asterisks (*) indicate a statistically significant differences in the volumes of the PBS-injected tumours compared to the virus-treated tumours. The number of tumours in each group were 10 (PBS), 11 (Ad5T122), and 12 (Ad5). Data are presented as mean ± standard error.</p

Schematic illustration of the virus constructs used in this study.

<p>Ad5 is a wild-type serotype 5 adenovirus containing an unmodified E1 region. In Ad5T122, six copies of miR122 target elements were introduced in the 3′UTR of E1A gene. Ad5Luc1 is a replication-deficient virus in which the whole E1-region has been replaced with a CMV-driven firefly luciferase gene.</p

Comparison of cell killing by Ad5 and Ad5T122 in a panel of cancer cell lines.

<p>Four cell lines of non-hepatic (HCT116, A549, and Hep-2) or hepatic (Huh7) origin were infected with Ad5Luc1 (non-replicative virus control), Ad5, or Ad5T122 at an MOI of 0.05. Cell survival in the infected cells was measured 7 days (Hep-2 and A549) or 9 days (HCT116 and Huh7) post-infection using an ATP-based cell viability assay, and plotted on the y-axis as the percentage of the control values measured from uninfected cultures. The data are presented as the mean of six repetitions ± standard error.</p