2 research outputs found
Strong Plasmonic Enhancement of a Single Peridinin–Chlorophyll <i>a</i>–Protein Complex on DNA Origami-Based Optical Antennas
In
this contribution, we fabricate hybrid constructs based on a
natural light-harvesting complex, peridinin–chlorophyll <i>a</i>–protein, coupled to dimer optical antennas self-assembled
with the help of the DNA origami technique. This approach enables
controlled positioning of individual complexes at the hotspot of the
optical antennas based on large, colloidal gold and silver nanoparticles.
Our approach allows us to selectively excite the different pigments
present in the harvesting complex, reaching a fluorescence enhancement
of 500-fold. This work expands the range of self-assembled functional
hybrid constructs for harvesting sunlight and can be further developed
for other pigment–proteins and proteins
Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer
Single-molecule localization
based super-resolution microscopy
has revolutionized optical microscopy and routinely allows for resolving
structural details down to a few nanometers. However, there exists
a rather large discrepancy between lateral and axial localization
accuracy, the latter typically three to five times worse than the
former. Here, we use single-molecule metal-induced energy transfer
(smMIET) to localize single molecules along the optical axis, and
to measure their axial distance with an accuracy of 5 nm. smMIET relies
only on fluorescence lifetime measurements and does not require additional
complex optical setups