DGAT1 overexpression is associated with an increased mitochondrial function and biogenesis in HFD-fed rats.

<p>(<b>A</b>) Western blot analysis of PGC1α (<b>B</b>) OXPHOS complexes and (<b>C</b>) UCP3 in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). <b>^#</b> P<0.05 HFD-DGAT1 vs. HFD-control.</p

DGAT1 overexpression results in bigger lipid droplets in HFD-fed rats.

<p>(<b>A</b>) Electron microscopy revealed an increase in lipid droplet diameter in DGAT1 overexpressing TA muscle of HFD-fed rats. (<b>B</b>) Western blot analysis of OXPAT in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12).</p

Three weeks of high-fat feeding is not associated with an increased TA ceramide content compared to rats on CHOW.

<p>DGAT1 overexpression does not result in an increase in TA ceramide content in rats on CHOW or HFD. Data are expressed as mean ± SEM (n = 10–12).</p

Three weeks of high-fat feeding is associated with an increased TA DAG content compared to rats on CHOW.

<p>DGAT1 overexpression leads to an increase in TA DAG content in rats on CHOW and tended to increase DAG content in rats on HFD. Data are expressed as mean ± SEM (n = 6). *P<0.05 CHOW-DGAT1 and HFD-control vs. CHOW-control.</p

Three weeks of high-fat feeding compared to rats on CHOW decreased whole-body insulin sensitivity as assessed by hyperinsulinemic eulgycemic clamp.

<p>DGAT1 overexpression in TA muscle increased muscle specific insulin sensitivity both in rats on CHOW as HFD. (<b>A</b>) Steady-state whole-body glucose infusion rates in rats on CHOW and HFD for three weeks. (<b>B</b>) 2DG uptake in TA muscle of CHOW fed rats after bolus injection of 2-deoxy-[3H]glucose under the same clamping conditions as in A. (<b>C</b>) 2DG uptake in TA muscle of HFD fed rats after bolus injection of 2-deoxy-[3H]glucose under the same clamping conditions as in A. Data are expressed as mean ± SEM (n = 10–12). *P<0.05 CHOW-DGAT1 and HFD-control vs. CHOW-control, <b>^#</b> P<0.05 HFD-DGAT1 vs. HFD-control.</p

The turnover of DAG and TAG is increased in the DGAT1 overexpressing TA muscle.

<p>(<b>A</b>) Western blotting of ATGL, (<b>B</b>) CGI58 (<b>C</b>) and ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). <b>^#</b> P<0.05 HFD-DGAT1 vs. HFD-control.</p