Development and Survivorship of Immature Angoumois Grain Moth (Lepidoptera: Gelechiidae) on Stored Corn

Life history of immature Angoumois grain moths, Sitotroga cerealella (Olivier), was studied on dent corn (Pioneer 3320) at 10, 15, 20, 25, 30, 35, and 40°C and at 43, 53-61, 75-76, and 82-87% RH under laboratory conditions. At 10 and 40°C, none of the stages survived at any relative humidity. Temperature was the main factor affecting egg incubation period, larval-pupal development time, and egg and larval-pupal survivorship. The shortest egg development times occurred at temperatures of 30°C and higher, but they increased sharply as temperature decreased. Larval-pupal development time was shortest at 30°C. Survivorship was optimal at 20-30°C for eggs and larvae-pupae, but larval-pupal survivorship decreased sharply at 15 and 35°C. Duration of larval-pupal development did not vary with sex. Newly emerged females were twofold heavier than males, and temperature and relative humidity did not affect weight. Sex ratio of emerging adults did not differ from 1:1 at any temperature or relative humidity. The optimum conditions for development of Angoumois grain moth on corn were 30°C and 75% RH. The data will be useful for determining safe storage conditions for corn and for developing a computer model for simulating population dynamics of immature S. cerealella

Effects of Rearing Density, Age, Sex, and Food Deprivation on Flight Initiation of the Red Flour Beetle (Coleoptera: Tenebrionidae)

Effects of rearing density, adult density and sex ratio in the flight chamber, adult age, sex, presence or absence of food, and duration of food deprivation on rate of and time to flight initiation of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), were studied in the laboratory. Rates of flight initiation were slightly lower at lower rearing densities, but they did not differ with age or sex of adults, presence or absence of food in the flight chamber, or duration of food deprivation. Focal adults were less likely to fly when individuals of the opposite sex were present in flight chambers. Presence of the same sex or mixed sexes and numbers of individuals in flight chambers did not affect tendency to fly of focal individuals. Mean time to flight of older beetles (7-20 d old) was less than that of younger beetles (1-4 d old). No young beetles flew during the first 24 h of flight tests. Mean time to flight did not differ with rearing density, sex, presence or absence of food, or duration of food deprivation. Our results indicate that this species is highly dispersive during the adult stage and that flight does not seem to be associated with just prereproductive or postreproductive dispersal phases, high rearing density, or short to medium periods of food deprivation. Therefore, T. castaneum level of flight activity does not seem to be associated with factors that have been shown in the literature to increase dispersal by walking for this species and to increase flight initiation in other stored-product species

Effect of Abiotic Factors on Initiation of Red Flour Beetle (Coleoptera: Tenebrionidae) Flight

Traps baited with pheromones are used to monitor the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), populations in flour mills to aid in making pest management decisions, but the factors that influence T. castaneum movement are not fully understood. We investigated the impact of photoperiod, light intensity, temperature, and relative humidity on flight initiation. The percentage of adults initiating flight reached a maximum at 30-35°C, and then fell to zero at 22.5 and 45°C. Only 2% of beetles flew in complete darkness, and the number of beetles initiating flight increased to 41% under 18 h of light and then decreased slightly to 37% under 24 h of light. Rates of flight initiation did not vary with light intensities from 1,784 to 4,356 lux or relative humidities from 25 to 85%. Thus, temperature and photoperiod are the main abiotic factors tested that impact flight initiation in T. castaneum, which have broad ranges of temperatures and photoperiods over which they can fly. The current results should be useful in helping to interpret trap catches based on abiotic conditions during the trapping period, and the results should be useful in helping to understand T. castaneum movement outside grain storages and processing facilities and their potential to infest structures

Effects of Rearing Density, Age, Sex, and Food Deprivation on Flight Initiation of the Red Flour Beetle (Coleoptera: Tenebrionidae)

Effects of rearing density, adult density and sex ratio in the flight chamber, adult age, sex, presence or absence of food, and duration of food deprivation on rate of and time to flight initiation of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), were studied in the laboratory. Rates of flight initiation were slightly lower at lower rearing densities, but they did not differ with age or sex of adults, presence or absence of food in the flight chamber, or duration of food deprivation. Focal adults were less likely to fly when individuals of the opposite sex were present in flight chambers. Presence of the same sex or mixed sexes and numbers of individuals in flight chambers did not affect tendency to fly of focal individuals. Mean time to flight of older beetles (7-20 d old) was less than that of younger beetles (1-4 d old). No young beetles flew during the first 24 h of flight tests. Mean time to flight did not differ with rearing density, sex, presence or absence of food, or duration of food deprivation. Our results indicate that this species is highly dispersive during the adult stage and that flight does not seem to be associated with just prereproductive or postreproductive dispersal phases, high rearing density, or short to medium periods of food deprivation. Therefore, T. castaneum level of flight activity does not seem to be associated with factors that have been shown in the literature to increase dispersal by walking for this species and to increase flight initiation in other stored-product species

Chronological Age-Grading of Three Species of Stored-Product Beetles by Using Near-Infrared Spectroscopy

The accuracy of near-infrared spectroscopy (NIRS) for predicting the chronological age of adults of the rice weevil, Sitophilus oryzae (L.); the lesser grain borer, Rhyzopertha dominica (F.); and the red flour beetle, Tribolium castaneum (Herbst), three pests of stored grain, was examined. NIRS-predicted age correlated well with actual age of these three species. Age predictions in S. oryzae by using the NIRS method are not dependent upon adult sex or temperatures to which adult weevils are exposed. Results indicated that water content decreased with increasing age in rice weevil adults, and excluding wavelengths at which water absorbs NIR radiation reduced the accuracy of correct classification. Additionally, removing cuticular lipids from insects resulted in a significant decrease in classification accuracy of weevils, indicating that these compounds may be partly responsible for the ability of NIRS to differentiate young from old beetles. NIRS is a nondestructive technique that can be used to age-grade large numbers of adult stored-product beetles, information that could help to increase the accuracy of population models for these pest species

Diseño de un Relleno Sanitario manual en el distrito de Jepelacio, San Martín

El objetivo de la presente investigación fue Diseñar un relleno sanitario manual para la disposición final de los residuos sólidos en el distrito de Jepelacio - San Martín. Para ello se desarrolló las siguientes actividades: caracterización los residuos sólidos, cálculo del área y volumen del relleno, selección de sitio y cálculo de la magnitud del impacto ambiental del relleno sanitario. La investigación tuvo un diseño no experimental transversal de tipo descriptivo. Se obtuvo una generación promedio de residuos sólidos municipales (domiciliarios y no domiciliarios) en los 15 años de 3.518 ton/día. El volumen acumulado de 46,239.122 m3 y requiriendo un área aproximada de 2.384ha para una vida útil de 15 años. La alternativa seleccionada para la construcción del relleno, presenta una distancia mayor a 1 km de la vivienda más cercana, no presenta riesgo de inundaciones, además de cumplir con todos los requisitos técnicos establecidos en la normativa nacional. Asimismo, en la evaluación del impacto ambiental del relleno se observa impactos tanto positivos como negativos en las etapas de construcción operación y cierre. Se concluye que la propuesta de relleno sanitario manual servirá a los gestores municipales para tomar decisiones en cuanto a manejo adecuado de los residuos sólidos.TesisTARAPOTOEscuela Profesional de Ingeniería AmbientalSaneamiento Ambiental y Tratamiento de Agua

Insect Fragments in Flour: Relationship to Lesser Grain Borer (Coleoptera: Bostrichidae) Infestation Level in Wheat and Rapid Detection Using Near-Infrared Spectroscopy

We determined that the number of insect fragments, quantified using the standard flotation method, in flour milled from wheat infested with larvae, pupae, or preemergent adults of the lesser grain borer, Rhyzopertha dominica (F.), was proportional to infestation level. Wheat infested with a single preemergent adult contributed 28 and 10X as many fragments as wheat infested with a single larva or pupa, respectively. Using regression models that were developed from these data, we predicted that the maximum infestation level that would result in flour with fragment counts below the Food and Drug Administration defect action level (75 fragments/50 g of flour) was 0.95 and 1.5% (380-640 infested kernels/kg of wheat) for pupae and larvae, but it decreased to 0.05% (20 infested kernels/kg) when the grain was infested with preemergent adults. We also reexamined the accuracy and sensitivity of near-infrared spectroscopy (NIRS) for detecting insect fragments in flour by testing three different NIR spectrometers. NIRS-predicted numbers of insect fragments were correlated with the actual number of fragments. NIRS is less precise than the standard flotation method, but it is rapid, nondestructive, does not require extensive sample preparation, and could easily be automated for a more sophisticated sampling protocol for flour based on prescreening samples with NIRS followed up by use of the standard flotation method when necessary

Detection of Stored-Grain Insect Infestation in Wheat Transported in Railroad Hopper-Cars

Levels of insect infestation, insect spatial distribution, and the relationship between the number of insect-damaged kernels (IDK) and the number of insects present in grain samples in three-hopper railcars transporting wheat from country elevators to a mill were studied. Six of eight sampled railcars were infested with more than two species of insects. The most abundant species collected were the lesser grain borer, Rhyzopertha dominica (F.), and rusty grain beetle, Cryptolestes ferrugineus (Stephens), with the larval stage of the two species being the most prevalent (\u3e90%). The spatial distributions of these two species within the grain mass were typically clumped in railcar compartments containing\u3e0.4 insect/2.75-kg sample of wheat, and these foci of high-infestation levels varied in compartments within the railcars and among the sampled railcars. There were no significant correlations between IDK and insect density for any of the different stage-specific insect populations that were collected in the grain samples. Mean numbers of immatures and IDK differed among railcars and compartments within railcars, but not among grain depths. Number of insects in the first discharge sample was not correlated with mean numbers of insects in the entire compartment. This indicates that each compartment of a railcar should be sampled to determine level of insect infestation but that sampling at different depths within a compartment is less important

Detection of Stored-Grain Insect Infestation in Wheat Transported in Railroad Hopper-Cars

Levels of insect infestation, insect spatial distribution, and the relationship between the number of insect-damaged kernels (IDK) and the number of insects present in grain samples in three-hopper railcars transporting wheat from country elevators to a mill were studied. Six of eight sampled railcars were infested with more than two species of insects. The most abundant species collected were the lesser grain borer, Rhyzopertha dominica (F.), and rusty grain beetle, Cryptolestes ferrugineus (Stephens), with the larval stage of the two species being the most prevalent (\u3e90%). The spatial distributions of these two species within the grain mass were typically clumped in railcar compartments containing\u3e0.4 insect/2.75-kg sample of wheat, and these foci of high-infestation levels varied in compartments within the railcars and among the sampled railcars. There were no significant correlations between IDK and insect density for any of the different stage-specific insect populations that were collected in the grain samples. Mean numbers of immatures and IDK differed among railcars and compartments within railcars, but not among grain depths. Number of insects in the first discharge sample was not correlated with mean numbers of insects in the entire compartment. This indicates that each compartment of a railcar should be sampled to determine level of insect infestation but that sampling at different depths within a compartment is less important

Rapid Assessment of Insect Fragments in Flour Milled from Wheat Infested with Known Densities of Immature and Adult \u3ci\u3eSitophilus oryzae\u3c/i\u3e (Coleoptera: Curculionidae)

Milling wheat, Triticum aestivum L., infested with low densities of internal feeding insects can result in flour containing insect fragments. The Food and Drug Administration (FDA) enforces a standard or defect action level stating that a maximum of 75 insect fragments per 50 g of flour is allowed. However, the relationship between level of infestation and number of resulting fragments is not well documented, and a more rapid method for enumerating insect fragments is needed. We characterized the number of insect fragments produced from milling small lots of wheat spiked with known densities and life stages of Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Insect fragments were enumerated with near-infrared spectroscopy (NIRS), a quick nondestructive procedure, and with the industry standard flotation method. Results showed that an individual small larva, large larva, pupa, or adult produced 0.4, 0.7, 1.5, and 27.0 fragments, respectively. NIRS-predicted counts of ≤51 (from small larvae), ≤53 (from large larvae), ≤43 (from pupae), or 0 (from adults) indicated that there weresample, because the upper bound of associated 95% inverse prediction confidence intervals was less than the standard; NIRS-predicted counts of ≥98, ≥117, ≥108, or ≥225 fragments (same life stages as above) signaled that these flour samples contained \u3e75 actual fragments. These data suggest that NIRS could be adopted for rapid assessment of insect fragments resulting from relatively low levels of infestation with immature life states, but that it was not accurate enough for enumerating insect fragments, relevant to FDA standards, resulting from adults