Transcriptome assembly of RNA-Seq data from field samples collected during wheat blast epidemic in Bangladesh 2016

In 2016, wheat fields in Bangladesh were infected by wheat blast fungus (<i>Magnaporthe oryzae</i>) for the first time. The fungus spread quickly thoroughout the country and threatened other wheat growing areas in neighbouring countries. Islam <i>et al </i>(BMC Biology 2016 14:84<strong>, </strong>DOI<strong>: </strong>10.1186/s12915-016-0309-7) collected infected wheat samples, extracted RNA, and obtained short reads by Illumina RNA-Seq technology. Here, we report assemby of these short reads using Trinity v 2.0.6.<br

Transcriptome sequencing of infected wheat samples collected during the wheat blast epidemic in Bangladesh in 2017 and release of raw sequence data on OpenWheatBlast website with open access

Wheat fields in Bangladesh were again infected by wheat blast fungus (<i>Magnaporthe oryzae</i>) in 2017 growing season, following the epidemic of 2016. Infected wheat samples were collected by Tofazzal Islam and team, and sent to the Sainsbury Laboratory for RNA extraction and sequencing. Library preparation and RNA-Seq sequencing runs were performed on Illumina HiSeq-2500 machines at the Earlham Institute to produce paired-end reads with ~267 bp average insert size. Here we report the release of these data to general public with open access on OpenWheatBlast website.<br

Transcriptome sequencing of rice leaves with blast symptoms collected from rice fields of the Philippines in 2017 and release of raw sequence data on OpenRiceBlast website for open access

<div>Infected rice samples were collected by Bo Zhou and team, and sent to the Sainsbury Laboratory for RNA extraction and sequencing. Library preparation and RNA-Seq sequencing runs were performed by Genewiz using Illumina HiSeq-2500 machines to produce paired-end reads with ~300 bp average insert size. Here we report the release of these data to general public with open access on OpenRiceBlast website.</div><div><br></div

Transcriptome sequencing of infected wheat samples collected during the wheat blast epidemic in Bangladesh in 2016 and release of raw sequence data on OpenWheatBlast website for open access

In 2016, wheat fields in Bangladesh were infected by wheat blast fungus (<i>Magnaporthe oryzae</i>) for the first time. The fungus spread quickly thoroughout the country and threatened other wheat growing areas in neighbouring countries. Islam <i>et al </i>(BMC Biology 2016 14:84<strong>, </strong>DOI<strong>: </strong>10.1186/s12915-016-0309-7) collected infected wheat samples, extracted RNA, and obtained short reads by Illumina RNA-Seq technology. Library preparation and sequencing runs were performed on Illumina HiSeq-2500 machines to produce paired-end reads with ~300 bp insert size. Here we report the release of all raw sequence data to public via OpenWheatBlast website for open access.<br><div><br></div

Consensus mapping of transcripts from RNA-Seq short read data of wheat samples collected during 2016 wheat blast epidemic in Bangladesh

This data set describes the synthesis of transcripts derived from RNA-Seq short read data from 2016 Bangladesh wheat blast epidemic openly available from "OpenWheatBlast" website. We aligned the short reads to the predicted transcriptome of Brazilian wheat blast isolate BR32 reported by <a href="http://gbe.oxfordjournals.org/content/7/10/2896">Chiapello et al. GBE (2016)</a> and obtained from the <a href="http://genome.jouy.inra.fr/gemo/">GEMO database</a>. Then, we extracted the predicted consensus sequences for the Bangladeshi strain(s). Here, we provide fasta files for these predicted consensus sequences based on the Br32 gene models

Transcriptome sequencing of rice leaves with blast symptoms collected from rice fields of Bangladesh in 2017 and release of raw sequence data on OpenRiceBlast website for open access

<div>Infected rice samples were collected by Tofazzal Islam and team, and sent to the Sainsbury Laboratory for RNA extraction and sequencing. Library preparation and RNA-Seq sequencing runs were performed by Genewiz using Illumina HiSeq-2500 machines to produce paired-end reads with ~300 bp average insert size. Here we report the release of these data to general public with open access on OpenRiceBlast website.</div><div><br></div

Plant probiotic bacteria suppress wheat blast fungus Magnaporthe oryzae Triticum pathotype

<b>Biological control of fearsome wheat blast by bacterial antagonist from native environment.</b

Genome sequences of candidate wheat blast biocontrol bacteria

In an effort to combat wheat blast disease in Bangladesh, Prof. Tofazzal Islam and team have identified several biocontrol bacteria that have the ability to inhibit fungal growth in wheat (Surovy et al., 2017). They have isolated a number of these agents and we have sequenced the genomes of four bacterial strains to 30x coverage. The genome sequence data is now available to download from links in the tables included in the document.<br