Sourcing obsidian: a new optimized LA-ICP-MS protocol

Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry [LA-ICP-MS] is one of the most successful analytical techniques used in archaeological sciences. Applied to the sourcing of lithic raw materials, it allows for fast and reliable analysis of large assemblages. However, the majority of published studies omit important analytical issues commonly encountered with laser ablation. This research presents a new advanced LA-ICP-MS protocol developed at Southern Cross GeoScience (SOLARIS laboratory, Southern Cross University, Australia), which optimizes the potential of this cutting-edge geochemical characterization technique for obsidian sourcing. This new protocol uses ablation lines with a reduced number of assayed elements (specific isotopes) to achieve higher sensitivity as well as increased precision and accuracy, in contrast to previous studies working with ablation points and an exhaustive list of measured isotopes. Applied to obsidian sources from the Western Mediterranean region, the Carpathian basin, and the Aegean, the results clearly differentiate between the main outcrops, thus demonstrating the efficiency of the new advanced LA-ICP-MS protocol in answering fundamental archaeological questions

Owning humankind : fossils, humans and archaeological remains

Abstract: There are a myriad of laws, guidelines and unwritten agreements relating to human, hominid and hominin remains. Legal gaps and inadequate definitions of what constitutes a fossil have meant that a ‘finders keepers’ approach is often applied to the ownership and control of our ancestors' remains. Such shortcomings expose numerous legal and ethical conundrums. Should any one organisation, individual or government control access to recently-found remains, limiting opportunities to unlock the secrets of evolution? Given that humans can start fossilisation processes immediately after burial, at what point does it become appropriate to dig up their remains? And who should control access to them? Could any prehistoric Homo ever have imagined they would one day be exhumed and their remains laid out in cases as the centrepiece of a museum exhibit? This paper surveys a number of implications that arise from these foundational questions, and ultimately challenges the belief that human, hominin and hominid remains are self-evident ‘objects’ capable of clear ownership: rather they constitute creative cultural intersections, which are deserving of greater ethical consideration. Protocols for respecting, protecting and conserving remains while allowing a greater equity in access to information about our common ancestors are both desirable and urgently required

A marine reservoir correction for the Houtman-Abrolhos Archipelago, East Indian Ocean, Western Australia

High-precision analysis using accelerator mass spectrometry (AMS) was performed upon known-age Holocene and modern, pre-bomb coral samples to generate a marine reservoir age correction value (ΔR) for the Houtman-Abrolhos Archipelago (28.7°S, 113.8°E) off the Western Australian coast. The mean ΔR value calculated for the Abrolhos Islands, 54 ± 30 yr (1σ) agrees well with regional ΔR values for Leeuwin Current source waters (N-NW Australia-Java) of 60 ± 38 yr. The Abrolhos Islands show little variation with ΔR values of the northwestern and north Australian coast, underlining the dominance of the more equilibrated western Pacific-derived waters of the Leeuwin Current over local upwelling. The Abrolhos Islands ΔR values have remained stable over the last 2896 cal yr BP, being also attributed to the Leeuwin Current and the El Niño Southern Oscillation (ENSO) signal during this period. Expected future trends will be a strengthening of the teleconnection of the Abrolhos Islands to the climatic patterns of the equatorial Pacific via enhanced ENSO and global warming activity strengthening the Leeuwin Current. The possible effect upon the trend of future ΔR values may be to maintain similar values and an increase in stability. However, warming trends of global climate change may cause increasing dissimilarity of ΔR values due to the effects of increasing heat stress upon lower-latitude coral communities

Extinction of eastern Sahul megafauna coincides with sustained environmental deterioration

Abstract: Explanations for the Upper Pleistocene extinction of megafauna from Sahul (Australia and New Guinea) remain unresolved. Extinction hypotheses have advanced climate or human-driven scenarios, in spite of over three quarters of Sahul lacking reliable biogeographic or chronologic data. Here we present new megafauna from north-eastern Australia that suffered extinction sometime after 40,100 (±1700) years ago. Megafauna fossils preserved alongside leaves, seeds, pollen and insects, indicate a sclerophyllous forest with heathy understorey that was home to aquatic and terrestrial carnivorous reptiles and megaherbivores, including the world’s largest kangaroo. Megafauna species diversity is greater compared to southern sites of similar age, which is contrary to expectations if extinctions followed proposed migration routes for people across Sahul. Our results do not support rapid or synchronous human-mediated continental-wide extinction, or the proposed timing of peak extinction events. Instead, megafauna extinctions coincide with regionally staggered spatio-temporal deterioration in hydroclimate coupled with sustained environmental change

Two Late Pleistocene human femora from Trinil, Indonesia: Implications for body size and behavior in Southeast Asia

Late Pleistocene hominin postcranial specimens from Southeast Asia are relatively rare. Here we describe and place into temporal and geographic context two partial femora from the site of Trinil, Indonesia, which are dated stratigraphically and via Uranium-series direct dating to ca. 37–32 ka. The specimens, designated Trinil 9 and 10, include most of the diaphysis, with Trinil 9 being much better preserved. Microcomputed tomography is used to determine cross-sectional diaphyseal properties, with an emphasis on midshaft anteroposterior to mediolateral bending rigidity (Ix/Iy), which has been shown to relate to both body shape and activity level in modern humans. The body mass of Trinil 9 is estimated from cortical area and reconstructed length using new equations based on a Pleistocene reference sample. Comparisons are carried out with a large sample of Pleistocene and Holocene East Asian, African, and European/West Asian femora. Our results show that Trinil 9 has a high Ix/Iy ratio, most consistent with a relatively narrow-bodied male from a mobile hunting-gathering population. It has an estimated body mass of 55.4 kg and a stature of 156 cm, which are small relative to Late Pleistocene males worldwide, but larger than the penecontemporaneous Deep Skull femur from Niah Cave, Malaysia, which is very likely female. This suggests the presence of small-bodied active hunter-gatherers in Southeast Asia during the later Late Pleistocene. Trinil 9 also contrasts strongly in morphology with earlier partial femora from Trinil dating to the late Early-early Middle Pleistocene (Femora II–V), and to a lesser extent with the well-known complete Femur I, most likely dating to the terminal Middle-early Late Pleistocene. Temporal changes in morphology among femoral specimens from Trinil parallel those observed in Homo throughout the Old World during the Pleistocene and document these differences within a single site

Characterization of acute kidney injury in critically ill patients with severe coronavirus disease 2019

Abstract Background Coronavirus disease 2019 (COVID-19)-associated acute kidney injury (AKI) frequency, severity and characterization in critically ill patients has not been reported. Methods Single-centre cohort performed from 3 March 2020 to 14 April 2020 in four intensive care units in Bordeaux University Hospital, France. All patients with COVID-19 and pulmonary severity criteria were included. AKI was defined using Kidney Disease: Improving Global Outcomes (KDIGO) criteria. A systematic urinary analysis was performed. The incidence, severity, clinical presentation, biological characterization (transient versus persistent AKI; proteinuria, haematuria and glycosuria) and short-term outcomes were evaluated. Results Seventy-one patients were included, with basal serum creatinine (SCr) of 69 ± 21 µmol/L. At admission, AKI was present in 8/71 (11%) patients. Median [interquartile range (IQR)] follow-up was 17 (12–23) days. AKI developed in a total of 57/71 (80%) patients, with 35% Stage 1, 35% Stage 2 and 30% Stage 3 AKI; 10/57 (18%) required renal replacement therapy (RRT). Transient AKI was present in only 4/55 (7%) patients and persistent AKI was observed in 51/55 (93%). Patients with persistent AKI developed a median (IQR) urine protein/creatinine of 82 (54–140) (mg/mmol) with an albuminuria/proteinuria ratio of 0.23 ± 20, indicating predominant tubulointerstitial injury. Only two (4%) patients had glycosuria. At Day 7 after onset of AKI, six (11%) patients remained dependent on RRT, nine (16%) had SCr >200 µmol/L and four (7%) had died. Day 7 and Day 14 renal recovery occurred in 28% and 52%, respectively. Conclusion Severe COVID-19-associated AKI is frequent, persistent, severe and characterized by an almost exclusive tubulointerstitial injury without glycosuria

The age of homo naledi and associated sediments in the rising star cave, South Africa

New ages for flowstone, sediments and fossil bones from the Dinaledi Chamber are presented. We combined optically stimulated luminescence dating of sediments with U-Th and palaeomagnetic analyses of flowstones to establish that all sediments containing Homo naledi fossils can be allocated to a single stratigraphic entity (sub-unit 3b), interpreted to be deposited between 236 ka and 414 ka. This result has been confirmed independently by dating three H. naledi teeth with combined U-series and electron spin resonance (US-ESR) dating. Two dating scenarios for the fossils were tested by varying the assumed levels of222Rn loss in the encasing sediments: a maximum age scenario provides an average age for the two least altered fossil teeth of 253 +82/-70 ka, whilst a minimum age scenario yields an average age of 200 +70/-61 ka. We consider the maximum age scenario to more closely reflect conditions in the cave, and therefore, the true age of the fossils. By combining the US-ESR maximum age estimate obtained from the teeth, with the U-Th age for the oldest flowstone overlying Homo naledi fossils, we have constrained the depositional age of Homo naledi to a period between 236 ka and 335 ka. These age results demonstrate that a morphologically primitive hominin, Homo naledi, survived into the later parts of the Pleistocene in Africa, and indicate a much younger age for the Homo naledi fossils than have previously been hypothesized based on their morphologyWe would also like to thank the many funding agencies that supported various aspects of this work. In particular we would like to thank the National Geographic Society, the National Research Foundation and the Lyda Hill Foundation for significant funding of the discovery, recovery and initial analysis of this material. Further support was provided by ARC (DP140104282: PHGMD, ER, JK, HHW; FT 120100399: AH). The ESR dosimetry study undertaken by CENIEH and Griffith University has been supported by a Marie Curie International Outgoing Fellowship (under REA Grant Agreement n˚ PIOF-GA-2013–626474) of the European Union’s Seventh Framework Programme (FP7/2007-2013) and an Australian Research Council Future Fellowship (FT150100215). ESR and U-series dating undertaken at SCU were supported by ARC (DP140100919: RJB)

Direct dating of human remains

To understand human evolution, archaeologists require precise chronologies to compare and contrast the fossil collection. While indirect dating techniques of human occupation sites usually applied on sediments are somehow more precise than direct dating techniques, the accuracy of indirect dating is frequently poor. Direct dating of human remains older than 50 to 60 ka (radiocarbon limit) is limited to U-series and ESR techniques. To minimize the impact of direct dating on valuable archaeological samples, non-destructive U-series and ESR analysis have to be carried out using specifically designed protocols. Both methods are seriously compromised by the fact that teeth accumulate large amounts of uranium following their deposition in sediments. During the three years of this PhD, a 2D mapping protocol has been developed on the isotope distributions and elemental concentrations of uranium and thorium in fossil teeth using laser ablation ICP-MS. Isotopic maps of enamel and dentine show complex patterns that imply that systematic mapping of fragments would provide accurate insight for U-series and ESR internal dose assessment. A fossil Neanderthal tooth from Payre (France) showed negligible U-migration through the external enamel surface compared to the internal migration from the dentine, with great implications for ESR dating. Non-destructive ESR analyses are carried out on enamel fragments instead of powders to minimize the impact of analysis on samples. Nevertheless, the ESR spectra of fragments have a high angular dependency which complicates their study and the establishment of experimental protocols. During this PhD, new measuring protocols and analytical decomposition of ESR spectra have allowed to gain new insight on the composite nature of the signal. The development of comprehensive model describing the influence of several oriented and non-oriented CO2- radicals in the spectra with complex kinetics and transfer processes has shown that major age underestimation can be expected for most fossil tooth enamel. The new model suggests that fossils such as the Irhoud specimen or Broken Hill had their age underestimated by around 30%, propelling the Irhoud specimen amongst the oldest anatomically modem humans in Africa