Relative growth rates (RGRs) of seedlings of Berkut, Krichauff, Gladius and Drysdale over 0 to 7 days grown with no added NaCl (circles) or treated with 100 mM NaCl (triangles).

<p>(SE, n = 6 for Berkut and Krichauff, n = 4 for Gladius and Drysdale). RGRs of treated plants were significantly difference to RGRs of control plants (2 way ANOVA, p = 0.025).</p

Gene expression pattern for the four cultivars under control conditions.

<p>(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions during the first 3 days of growth under control conditions compared with the starting point (day 0) of the experiments. (B) Heat maps indicating intensity of gene expression in Berkut. Log FC = log₂ (the signal intensity under saline conditions / the signal intensity under control conditions). (C) Analysis of the gene ontology of 39 genes upregulated only in Berkut under control conditions. Functional categorizations by annotation were shown as gene ontology of biological process.</p

Additional file 1: Figure S1. of Genetic association of stomatal traits and yield in wheat grown in low rainfall environments

Frequency distribution of phenotypes for stomatal size related traits and yield in the RAC875/Kukri DH lines based on means obtained over each experiment. a) Lameroo, b) Roseworthy, c) Well watered conditions in the glasshouse, d) Drought conditions in the glasshouse. Arrows indicate phenotypic values of RAC875 (R) and Kukri (K). (PPTX 264 kb

Additional file 4: Figure S3. of Genetic association of stomatal traits and yield in wheat grown in low rainfall environments

Morphological features of a single stomata. Arrows indicate aperture length (APL) and width (APW) and guard cell length (GCL) and width (GCW). Aperture area (APA) and guard cell area (GCL) were calculated by multiplying the length and width of the rectangle. (DOCX 261 kb

Sequential monitoring of shoot biomass under conditions of salinity stress.

<p>Quantification of growth through time under conditions of salinity stress of (A) Berkut (blue diamonds, SE, n = 6), (B) Krichauff (orange triangles, SE, n = 6,), (C) Gladius (green squares, SE, n = 4) or (D) Drysdale (red circles, SE, n = 4). Cultivars were treated with RO water (open symbols) or 100 mM NaCl (filled symbols) at the emergence of the fourth leaf (vertical line indicates day 0 or day of treatment). Projected shoot area (pixels) was determined by image analysis from digital images taken with RGB cameras. Exponential curves were fitted to data from day 0 to day 7 after treatment (rectangle within graphs A to D) to calculate relative growth rates for (E) Berkut, (F) Krichauff, (G) Gladius and (H) Drysdale seedlings under control and saline conditions.</p

Venn diagram analyses of genes regulated in all four cultivars under salinity conditions (100 mM NaCl).

<p>(A–C) Venn diagrams showing a five-fold or greater difference in expression at 1 (A), 2 (B) or 3 (C) days after NaCl treatment. Red numeric characters show the number of upregulated genes. Blue numeric characters show the number of downregulated genes.</p

Comparative analysis of gene expression among regulated genes in Berkut under control conditions, in Krichauff under saline conditions and in Drysdale under saline conditions (100 mM NaCl).

<p>(A) Venn diagrams show the comparative analysis of upregulated gene expression associated with each specific physiological trait. (B) Venn diagrams show the comparative analysis of downregulated gene expression associated with each specific physiological trait.</p

Gene expression pattern for four cultivars under conditions of salinity stress.

<p>(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions under saline conditions (100 mM NaCl). Red numeric characters show the number of upregulated genes. Blue numeric characters show the number of downregulated genes. (B) Analysis of the gene ontology of 48 genes increased only in Krichauff under saline conditions. Functional categorizations by annotation were shown as gene ontology of biological process. (C) Analysis of the gene ontology of 21 genes increased only in Drysdale under saline conditions.</p