1 research outputs found
Antiviral Rotenoids and Isoflavones Isolated from <i>Millettia</i> <i>oblata</i> ssp. <i>teitensis</i>
Three new (1–3) and
six known
rotenoids (5–10), along with three
known isoflavones (11–13), were isolated
from the leaves of Millettia oblata ssp. teitensis. A new glycosylated isoflavone (4), four known isoflavones (14–18), and one known chalcone (19) were isolated from the
root wood extract of the same plant. The structures were elucidated
by NMR and mass spectrometric analyses. The absolute configuration
of the chiral compounds was established by a comparison of experimental
ECD and VCD data with those calculated for the possible stereoisomers.
This is the first report on the use of VCD to assign the absolute
configuration of rotenoids. The crude leaves and root wood extracts
displayed anti-RSV (human respiratory syncytial virus) activity with
IC50 values of 0.7 and 3.4 μg/mL, respectively. Compounds 6, 8, 10, 11, and 14 showed anti-RSV activity with IC50 values of
0.4–10 μM, while compound 3 exhibited anti-HRV-2
(human rhinovirus 2) activity with an IC50 of 4.2 μM.
Most of the compounds showed low cytotoxicity for laryngeal carcinoma
(HEp-2) cells; however compounds 3, 11,
and 14 exhibited low cytotoxicity also in primary lung
fibroblasts. This is the first report on rotenoids showing antiviral
activity against RSV and HRV viruses