, H4 cells stably overexpressing APP (H4-APP) were transfected with OSBP1 cDNA as described in Methods

Cell lysates from untransfected cells and those transiently overexpressing OSBP1 were immunoblotted with antibodies to the C-terminus of APP (upper panel), c-myc (middle panel), which detects the myc-tagged OSBP1, and actin, used for loading control. , OSBP1 overexpression decreased PMA-regulated sAPPα secreted levels. Cells were treated with PMA as described in Methods. Proteins from cell lysates and media were separated by SDS-PAGE and lysates were immunoblotted with anti-myc antibody (upper panel). Media samples were analyzed for sAPPα using the 6E10 antibody (middle panel). Cell lysates were also analyzed for CTFα using an antibody to the C-terminus of APP (lower panel). Densitometric analysis of Western blots is shown on the right. *p < 0.05 and **p < 0.01 by Student's test.<p><b>Copyright information:</b></p><p>Taken from "Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein"</p><p>http://www.molecularneurodegeneration.com/content/3/1/5</p><p>Molecular Neurodegeneration 2008;3():5-5.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2323375.</p><p></p

Cells were transfected with the 3 siRNA sequences as described in Methods

Non-targeting siRNA (NT) and siRNA against APP and BACE1 were used as controls. , Media samples were assayed for Aβ40 and Aβ42 by ELISA. , Lysates from HEK-APPNFEV cells were separated by SDS-PAGE and immunoblotted with an antibody to the APP C-terminus for detection of full length APP and APP-CTFs, and to actin as loading control. , Lysates from HEK-APPNFEV cells and immunoblotted with antibodies to OSBP1 and actin to confirm effective knockdown. *p < 0.01 and **p < 0.05 by ANOVA.<p><b>Copyright information:</b></p><p>Taken from "Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein"</p><p>http://www.molecularneurodegeneration.com/content/3/1/5</p><p>Molecular Neurodegeneration 2008;3():5-5.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2323375.</p><p></p