1 research outputs found
In Vivo Anti-Biofilm and Anti-Bacterial Non-Leachable Coating Thermally Polymerized on Cylindrical Catheter
Catheters
are indispensable tools of modern medicine, but catheter-associated
infection is a significant clinical problem, even when stringent sterile
protocols are observed. When the bacteria colonize catheter surfaces,
they tend to form biofilms making them hard to treat with conventional
antibiotics. Hence, there is a great need for inherently antifouling
and antibacterial catheters that prevent bacterial colonization. This
paper reports the preparation of nonleachable antibiofilm and antibacterial
cationic film coatings directly polymerized from actual tubular silicone
catheter surfaces via the technique of supplemental activator and
reducing agent surface-initiated atom-transfer radical polymerization
(SARA SI-ATRP). Three cross-linked cationic coatings containing (3-acrylamidopropyl)
trimethylammonium chloride (AMPTMA) or quaternized polyethylenimine
methacrylate (Q-PEI-MA) together with a cross-linker (polyethylene
glycol dimethacrylate, PEGDMA) were tested. The in vivo antibacterial
and antibiofilm effect of these nonleachable covalently linked coatings
(using a mouse catheter model) can be tuned to achieve 1.95 log (98.88%)
reduction and 1.26 log (94.51%) reduction of clinically relevant pathogenic
bacteria (specifically with methicillin-resistant Staphylococcus
aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VRE)). Our good in vivo bactericidal
killing results using the murine catheter-associated urinary tract
infection (CAUTI) model show that SARA SI-ATRP grafting-from technique
is a viable technique for making nonleachable antibiofilm coating
even on “small” (0.30/0.64 mm inner/outer diameter)
catheter