Additional file 2 of Moonlighting glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of Lactobacillus gasseri attenuates allergic asthma via immunometabolic change in macrophages

Additional file 2. Details of RNA-seq analysis

Additional file 1 of Moonlighting glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of Lactobacillus gasseri attenuates allergic asthma via immunometabolic change in macrophages

Additional file 1. Details of the identification of the anti-allergy fraction, IE3-3G1, from L. gasseri

Additional file 4 of Moonlighting glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of Lactobacillus gasseri attenuates allergic asthma via immunometabolic change in macrophages

Additional file 4. Raw data of sub-fraction IE3-3G1 proteomics analysis using LC–MS/MS

Additional file 3 of Moonlighting glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of Lactobacillus gasseri attenuates allergic asthma via immunometabolic change in macrophages

Additional file 3: Table S1. Experimental protocol of ion-exchange chromatography. Table S2. Experimental protocol of size-exclusion chromatography. Table S3. Data collection and refinement statistics of the LGp40 crystal. Figure S1. IL-12p40 levels of crude extracts used to stimulate mouse BMDC. Figure S2. Ion-exchange chromatography of crude extracts on a DEAE-Sepharose Fast Flow column. Figure S3. IL-12p40 levels of IE1-1 to IE4-2 fractions (fractions from ion-exchange chromatography) used to stimulate mouse BMDC. Figure S4. Size-exclusion chromatography of fractions from crude extract IE1 on a Sephacryl S-300 HR column. Figure S5. Size-exclusion chromatography of fractions from crude extract IE2 on a Sephacryl S-300 HR column. Figure S6. Size-exclusion chromatography of fractions from crude extract IE3 on a Sephacryl S-300 HR column. Figure S7. Identification and purification of sub-fraction IE3-3G1. Figure S8. GAPDH derived from probiotics and pathogens are dissimilar. Figure S9. RNA-seq analysis showed differentially regulated gene expression between LGp40 and CDp40-stimulated BMDM. Figure S10. The plasminogen interaction and plasmin activation ability of LGp40 decreased when the GAPDH activity was lost