106 research outputs found
Effects of Helium-implantation on Fracture Behavior of Reduced Activation Martensitic Steel F82H
Effect of Helium on Mechanical Properties of ODS Ferritic/Martensitic Steels for Fusion Applications
Evaluation of Fracture Toughness and Tensile Properties of Reduced Activation Materials with Miniaturized Specimen
Effect of Helium on Mechanical Properties of High Chromium ODS Ferritic/Martensitic Steels for Fusion Applications
Establishment of Small Specimen Test Technique for Fracture Toughness Evaluation of Reduced Activation Ferritic Steels towards Highly Efficient Utilization of High Energy Neutron Source
Cupricyclins, Novel Redox-Active Metallopeptides Based on Conotoxins Scaffold
Highly stable natural scaffolds which tolerate multiple amino acid substitutions represent the ideal starting point for the application of rational redesign strategies to develop new catalysts of potential biomedical and biotechnological interest. The knottins family of disulphide-constrained peptides display the desired characteristics, being highly stable and characterized by hypervariability of the inter-cysteine loops. The potential of knottins as scaffolds for the design of novel copper-based biocatalysts has been tested by engineering a metal binding site on two different variants of an ω-conotoxin, a neurotoxic peptide belonging to the knottins family. The binding site has been designed by computational modelling and the redesigned peptides have been synthesized and characterized by optical, fluorescence, electron spin resonance and nuclear magnetic resonance spectroscopy. The novel peptides, named Cupricyclin-1 and -2, bind one Cu2+ ion per molecule with nanomolar affinity. Cupricyclins display redox activity and catalyze the dismutation of superoxide anions with an activity comparable to that of non-peptidic superoxide dismutase mimics. We thus propose knottins as a novel scaffold for the design of catalytically-active mini metalloproteins
Microalgal food supplements from the perspective of Polish consumers: patterns of use, adverse events, and beneficial effects
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