2 research outputs found
Sigma-2 receptor ligand anchored telmisartan loaded nanostructured lipid particles augmented drug delivery, cytotoxicity, apoptosis and cellular uptake in prostate cancer cells
<p>Recently, the anticancer activity of telmisartan (TEL) has been discovered against prostate cancer. Nevertheless, despite favorable therapeutic profile, poor aqueous solubility and suboptimal oral bioavailability hamper the anticancer efficacy of TEL. Therefore, in this investigation, sigma-2 receptor ligand, 3-(4-cyclohexylpiperazine-1-yl) propyl amine (CPPA) anchored nanostructured lipid particles of telmisartan (CPPA-TEL-NLPs) were engineered using stearic acid for targeting prostate cancer, PC-3 cells. The mean particle size of TEL-NLPs was measured to be 25.4βΒ±β3.2βnm, significantly (<i>p</i>β<β0.05) lower than 32.6βΒ±β5.3βnm of CPPA-TEL-NLPs. Correspondingly, the zeta-potential of TEL-NLPs was measured to be β15.4βΒ±β2.3βmV significantly (<i>p</i>β<β0.05) higher than β9.6βΒ±β2.7βmV of CPPA-TEL-NLPs. The encapsulation efficiency of CPPA-TEL-NLPs was estimated to be 72.7βΒ±β4.3%, significantly (<i>p</i>β<β0.05) lower than 77.5βΒ±β5.4%, displayed by TEL-NLPs. In addition, FT-IR and PXRD confirmed the molecular encapsulation of the drug in amorphous state. <i>In vitro</i> drug release study was conducted to determine the drug delivery potential of tailored nanoparticles. TEL-NLPs released 93.36% of drug significantly (<i>p</i>β<β0.05) higher than 85.81%, released by CPPA-TEL-NLPs in 24βh. The IC<sub>50</sub> of CPPA-TEL-NLPs was measured to be 20.3βΒ΅M significantly (<i>p</i>β<β0.05) lower than 36.3βΒ΅M presented by TEL-NLPs in PC-3 cells. In contrast, CPPA-TEL-NLPs displayed the IC<sub>50</sub> of 41.3βΒ΅M, significantly (<i>p</i>β>β0.05) not different from 43.4βΒ΅M, exhibited by TEL-NLPs in PNT-2 cells. We elucidated that CPPA-TEL-NLPs entered the PC-3 cells via receptor mediated endocytosis pathway and thus exhibited superior cytotoxicity, apoptosis and greater extent of cellular uptake in PC-3 cells. In conclusion, CPPA-TEL-NLPs may be a promising nanomedicine and warrant further <i>in vivo</i> investigations for gaining clinical success.</p
Tetanus toxoid-loaded cationic non-aggregated nanostructured lipid particles triggered strong humoral and cellular immune responses
<p>In the present investigation, non-aggregated cationic and unmodified nanoparticles (TT-C-NLPs4 and TT-NLPs1) were prepared of about 49.2βΒ±β6.8-nm and 40.8βΒ±β8.3-nm, respectively. In addition, spherical shape, crystalline architecture and cationic charge were also noticed. Furthermore, integrity and conformational stability of TT were maintained in both TT-C-NLPs4 and TT-NLPs1, as evidenced by symmetrical position of bands and superimposed spectra, respectively in SDS-PAGE and circular dichroism. Cellular uptake in RAW264.7 cells indicating the concentration-dependent internalisation of nanoparticles. Qualitatively, CLSM exhibited enhanced cellular uptake of non-aggregated TT-C-NLPs4 owing to interaction with negatively charged plasma membrane and clevaloe mediated/independent endocytosis. In last, <i>in vivo</i> immunisation with non-aggregated TT-C-NLPs4 elicited strong humoral (anti-TT IgG) and cellular (IFN-Ξ³) immune responses at day 42, as compared to non-aggregated TT-NLPs1 and TT-Alum following booster immunisation at day 14 and 28. Thus, non-aggregated cationic lipid nanoparticles may be a potent immune-adjuvant for parenteral delivery of weak antigens.</p