Indares.com As an Instrument for Research and Educational Support

Contemporary education and research uses information and communication technologies on a large scale. The Indares (International Database for Research and Educational Support) system (http://www.indares.com) is a purposefully developed on-line server aimed at recording, analysing and comparing physical activity of the system users. The purpose of this internet-based project is to support education and research in the area of physical activity. Other aims are to increase the awareness of the system users about the issue of physical activity and to provide means to improve their lifestyle. The system is easily accessible to all users and is provided free of charge. The Indares system includes a total of six independent modules in six language versions. In this paper are presented the possibilities of their educational and research applications. This system has a great potential to support the educational and research process in the area of physical education and physical activity. Appropriate development and promotion of the system can result in a variety of applications in practical motivation for physical activity and effective research

Real-Time PCR Diagnostics Failure Caused by Nucleotide Variability within Exon 4 of the Human Cytomegalovirus Major Immediate-Early Gene

Here we report how variability in the human cytomegalovirus genome sequence may seriously affect the outcome of its molecular diagnosis. A real-time quantitative PCR assay targeting the major immediate-early gene failed to detect the viral load in some, but not all, clinical samples from hematooncological patients. By amplification and sequencing the DNA across the regions targeted by this assay we found a number of nucleotide substitutions which accounted for decreased primer/probe binding. This decreased the sensitivity of the assay up to 1,000-fold

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