Signature generation cost comparison.

<p>Signature generation cost comparison.</p

Performance comparisons with previous schemes.

<p>Performance comparisons with previous schemes.</p

Verifier’s computational cost comparison.

<p>Verifier’s computational cost comparison.</p

Ultrasonic-Assisted Extraction of Raspberry Seed Oil and Evaluation of Its Physicochemical Properties, Fatty Acid Compositions and Antioxidant Activities - Fig 3

<p>Three-dimensional surface and contour plots reflect the relationship of process variables on (a) extraction yield, (b) vitamin E content, and (c) antioxidant capacity of raspberry seed oil under ultrasonic-assisted extraction.</p

Inhibitory effect of extract from raspberry seed on H2O2-induced production of intracellular ROS of UAE and SE.

<p>RAW 264.7 macrophages were incubated in the presence or absence of H2O2. (A) The ROS levels in the macrophages were determined using fluorescence plate reader. The y axis of the ROS fluorescence represented the intensity of the fluorescent DCF in cell samples relative to unstained cells. The results are the means of three independent experiments. Bars with different alphabets are significantly different (P < 0.05). DC: H2O2-damaged group. (B) The ROS levels were monitored with laser scanning confocal microscope.</p

Effect of different solvent types on raspberry seed oil extraction under UAE.

<p>Effect of different solvent types on raspberry seed oil extraction under UAE.</p

Central composite design (CCD) for natural variable levels as well as experimental values of dependent responses of extraction yield and antioxidant capacity for raspberry seed oil using ultrasonic-assisted extraction.

<p>Central composite design (CCD) for natural variable levels as well as experimental values of dependent responses of extraction yield and antioxidant capacity for raspberry seed oil using ultrasonic-assisted extraction.</p

Gas chromatograph mass spectrometry (GC-MS) profile of essential oil from raspberry seed.

<p>Peak 1, Linoleic acid; Peak 2, γ-Linolenic acid; Peak 3, γ-Tocopherol; Peak 4, α-Tocopherol; Peak 5, γ-Sitosterol.</p

Fatty acid compositions of ultrasonic-assisted extracted oil (UAE) and soxhlet extracted (SE) raspberry seed oil.

<p>Fatty acid compositions of ultrasonic-assisted extracted oil (UAE) and soxhlet extracted (SE) raspberry seed oil.</p

Targeting and Microenvironment-Activated Nanoreactor for Diabetic Chronic Wound Healing via Multienzyme Cascade Reactions

The development of cell-like nanoreactors with the ability to initiate biocatalytic cascades under special conditions holds tremendous potential for therapeutic applications. Herein, conformationally gated nanoreactors that respond to the acidic microenvironment of infected diabetic wounds were developed by cucur[8]bituril (CB[8])-based supramolecular assembly. The bioinspired nanoreactors exhibit not only self-regulated permeability and selectivity to control internal enzyme activities by substance exchange but also distinct binding specificities toward Gram-positive and Gram-negative bacteria via noncovalent modification with different ligands. The encapsulation of glucose oxidase (GOx), Fe3O4 nanozyme, and l-arginine (l-Arg) into the nanocarriers enables intelligent activation of multienzyme cascade reactions upon glucose (Glu) uptake to produce gluconic acid (GA) and hydrogen peroxide (H2O2), which is further converted into highly toxic hydroxyl radicals (·OH) for selective antibacterial activity. Moreover, acidic H2O2 promotes the oxidization of l-Arg, leading to the release of nitric oxide (NO). Consequently, this nanoreactor provides a multifunctional and synergistic platform for diabetic chronic wound healing by combining enzyme dynamic therapy with NO gas therapy to combat bacterial infections and inflammation under high blood Glu levels