Over-expression of <i>STO</i> promotes <i>FT</i> and <i>SOC1</i> expression independent of <i>CO</i> or <i>CCA1.</i>

<p>The level of <i>CCA1</i> (A), <i>CO</i> (B), <i>FT</i> (C) and <i>SOC1</i> (D) expression in eight-day-old seedlings under SD conditions. Data from two independent replicates are shown, with <i>UBQ10</i> used as a control. White and black bars represent the light and dark periods, respectively.</p

<i>sto-1</i> and <i>STO-OE</i> show diverse phenotypes.

<p>Col, <i>sto-1</i> and <i>STO-OE</i> were grown under SD conditions and different growth characteristics analyzed. (A) Seed germination as a percentage of total seeds analyzed; (B) hypocotyl length; (C) and (E) Seedling growth as measured by average hypocotyl length (<i>n</i> = 20–30) after 5 d on1/2 MS plates treated with BL (0–1000 nM) in SD; (D) and (F) Seedling growth as measured by average hypocotyl length (<i>n</i> = 20–30) after treatment with ACC (0–10 µM) for 3.5 days in darkness; (G) Appearance of adult rosette leaves on both adaxial and abaxial surfaces, with arrows indicating the position of leaf serration; (H–K) Flowering time of Col, <i>sto-1</i> and <i>STO-OE</i> in LD and SD conditions. The average number of rosette leaves in LD (J) and SD (K) was also calculated (<i>n</i> = 15–28, * means p<0.05 and ** means p<0.01 in TTEST).</p

Over-expression of <i>STO</i> promotes <i>FT</i> and <i>SOC1</i> expression independent of <i>FLC</i> repression.

<p>The level of <i>STO</i> (A), <i>FT</i> (B) and <i>SOC1</i> (C) expression in eight-day-old seedlings of the indicated genotypes. In the analysis, the data from STO-OE/flc/FRI-4 and -15 were compared to that of flc/FRI, while the data for STO-OE/FLC/FRI-11 was compared to that of FRI. Data from three independent replicates are shown, with <i>UBQ10</i> used as a control. (D) The number of rosette leaves in each indicated genotype at the time of flowering. All seedlings and mature plants were grown in SD. ** means p<0.01 in TTEST.</p