3 research outputs found

    Media 2: 3D differential phase-contrast microscopy with computational illumination using an LED array

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    Originally published in Optics Letters on 01 March 2014 (ol-39-5-1326

    A Graphene-Based Sensor Array for High-Precision and Adaptive Target Identification with Ensemble Aptamers

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    In this work, we report a new concept of adaptive “ensemble aptamers” (ENSaptamers) that exploits the collective recognition abilities of a small set of rationally designed, nonspecific DNA sequences to identify molecular or cellular targets discriminatively. In contrast to in vitro-selected aptamers, which possess specific “lock-and-key” recognition, ENSaptamers rely on pattern recognition that mimics natural olfactory or gustatory systems. Nanographene oxide was employed to provide a low-background and highly reproducible fluorescent assay system. We demonstrate that this platform provides a highly discriminative and adaptive tool for high-precision identification of a wide range of targets for diagnostic and proteomic applications with a nearly unlimited supply of ENSaptamer receptors
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