The Association of Adiponectin Gene Promoter Variations with Non-Small Cell Lung Cancer in a Han Chinese Population

<div><p>Recently, in vitro studies have demonstrated that adiponectin has antiangiogenic and tumor growth-limiting properties. Additionally, serum adiponectin levels have been associated with the risk of several cancers; specifically, serum adiponectin was significantly lower in lung cancer patients with advanced-stage disease. In this study, we examined the association of adiponectin gene promoter variations associated with adiponectin gene expression and plasma levels in non-small cell lung cancer (NSCLC) in a Han Chinese population. A total of 319 patients with NSCLC and 489 healthy individuals were recruited to evaluate the association of four adiponectin gene promoter single-nucleotide polymorphisms (SNPs) (SNP-12140G>A, SNP-11426A>G, SNP-11391G>A and SNP-11377C>G) with NSCLS risk. Additionally, we constructed haplotypes of these four SNPs and evaluated the association of these haplotypes with NSCLS risk. Our results showed that among these four SNPs, only SNP-12140G>A was associated with NSCLC risk(P<0.05). The haplotype analysis showed that no haplotype was associated with NSCLC after performing a Bonferroni correction (P>0.05). Additionally, an association analysis of the four SNPs stratified into pathologic stages I+II and III+IV showed that these SNPs did not exhibit significant differences between pathologic stages I+II and III+IV. Moreover, we did not observe any differences in allele and genotype frequency for these SNPs between adenocarcinoma and squamous cell carcinoma. Our results indicated that the G allele of SNP-12140may be a risk factor for NSCLC (OR = 1.516; 95% CI: 1.098–2.094) in this Han Chinese population.</p></div

Different inheritance models analysis of the SNP-11377C>G in adiponectin gene promoter between NSCLC and control groups.

<p>Different inheritance models analysis of the SNP-11377C>G in adiponectin gene promoter between NSCLC and control groups.</p

Different haplotypes analysis of SNP-12140G>A, SNP-11426A>G, SNP-11377C>G in adiponectin gene promoter between NSCLC and control groups.

<p>Different haplotypes analysis of SNP-12140G>A, SNP-11426A>G, SNP-11377C>G in adiponectin gene promoter between NSCLC and control groups.</p

Clinical characteristics of the subjects enrolled in the present study (Data are mean±SD).

<p>Clinical characteristics of the subjects enrolled in the present study (Data are mean±SD).</p

Association analysis of the adiponectin promoter SNP-12140G>A, SNP-11426A>G, SNP-11377C>G between adenocarcinoma (AC) and squamous cell carcinoma (SCC).

<p>Association analysis of the adiponectin promoter SNP-12140G>A, SNP-11426A>G, SNP-11377C>G between adenocarcinoma (AC) and squamous cell carcinoma (SCC).</p

Comparison of genotypic and allelic distribution of SNP-12140G>A, SNP-11426A>G, SNP-11377C>G between pathologic stages I+II and III+IV.

<p>Comparison of genotypic and allelic distribution of SNP-12140G>A, SNP-11426A>G, SNP-11377C>G between pathologic stages I+II and III+IV.</p

Different inheritance models analysis of the SNP-11426A>G in adiponectin gene promoter between NSCLC and control groups.

<p>Different inheritance models analysis of the SNP-11426A>G in adiponectin gene promoter between NSCLC and control groups.</p