Weighted Total Least Squares of Universal EIV Adjustment Model

This paper proposes the universal errors-in-variables (EIV) adjustment model based on the fundamental adjustment theory, which covers the parametric adjustment model, conditional adjustment model,conditional adjustment model with parameters and parametric adjustment model with constrains. Applying total least squares (TLS) principle, we deduce the weighted TLS (WTLS) algorithm and the approximated precision of the EIV model. The universal EIV adjustment model and its estimator of WTLS contribute to the integrity of theory of EIV model estimation. The proposed uniform WTLS algorithm is appropriate for programming in software, which can contribute to the geodetic application of the theory of the EIV model estimation

Experimental and Theoretical Study on Dynamic Hydraulic Fracture

Hydraulic fracturing is vital in the stimulation of oil and gas reservoirs, whereas the dynamic process during hydraulic fracturing is still unclear due to the difficulty in capturing the behavior of both fluid and fracture in the transient process. For the first time, the direct observations and theoretical analyses of the relationship between the crack tip and the fluid front in a dynamic hydraulic fracture are presented. A laboratory-scale hydraulic fracturing device is built. The momentum-balance equation of the fracturing fluid is established and numerically solved. The theoretical predictions conform well to the directly observed relationship between the crack tip and the fluid front. The kinetic energy of the fluid occupies over half of the total input energy. Using dimensionless analyses, the existence of equilibrium state of the driving fluid in this dynamic system is theoretically established and experimentally verified. The dimensionless separation criterion of the crack tip and the fluid front in the dynamic situation is established and conforms well to the experimental data. The dynamic analyses show that the separation of crack tip and fluid front is dominated by the crack profile and the equilibrium fluid velocity. This study provides a better understanding of the dynamic hydraulic fracture

Mixed LS-TLS Estimation Based on Nonlinear Gauss-Helmert Model

For the case of design matrix in EIV(errors-in-variables) model containing both fixed elements and random elements, this paper proposes a mixed LS-TLS(least squares-total least squares) algorithm and deduces the precision estimator by reformulating an EIV model as a nonlinear Gauss-Helmert model, in which random elements are extracted to the random model of adjustment. This algorithm can be applied to the general design matrix including simultaneously fixed columns, fixed elements and random elements. The example illustrates that the solution of mixed LS-TLS equal the solution of structured or weighted TLS algorithms which can solve mixed LS-TLS problem. Additionally, the solution of mixed LS-TLS statistically superior to solution of LS or TLS

Mitochondrial genomes of blister beetles (Coleoptera, Meloidae) and two large intergenic spacers in Hycleus genera

Abstract Background Insect mitochondrial genomes (mitogenomes) exhibit high diversity in some lineages. The gene rearrangement and large intergenic spacer (IGS) have been reported in several Coleopteran species, although very little is known about mitogenomes of Meloidae. Results We determined complete or nearly complete mitogenomes of seven meloid species. The circular genomes encode 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs) and two ribosomal RNAs (rRNAs), and contain a control region, with gene arrangement identical to the ancestral type for insects. The evolutionary rates of all PCGs indicate that their evolution is based on purifying selection. The comparison of tRNA secondary structures indicates diverse substitution patterns in Meloidae. Remarkably, all mitogenomes of the three studied Hycleus species contain two large intergenic spacers (IGSs). IGS1 is located between trnW and trnC, including a 9 bp consensus motif. IGS2 is located between trnS2 (UCN) and nad1, containing discontinuous repeats of a pentanucleotide motif and two 18-bp repeat units in both ends. To date, IGS2 is found only in genera Hycleus across all published Coleopteran mitogenomes. The duplication/random loss model and slipped-strand mispairing are proposed as evolutionary mechanisms for the two IGSs (IGS1, IGS2). The phylogenetic analyses using MrBayes, RAxML, and PhyloBayes methods based on nucleotide and amino acid datasets of 13 PCGs from all published mitogenomes of Tenebrionoids, consistently recover the monophylies of Meloidae and Tenebrionidae. Within Meloidae, the genus Lytta clusters with Epicauta rather than with Mylabris. Although data collected thus far could not resolve the phylogenetic relationships within Meloidae, this study will assist in future mapping of the Meloidae phylogeny. Conclusions This study presents mitogenomes of seven meloid beetles. New mitogenomes retain the genomic architecture of the Coleopteran ancestor, but contain two IGSs in the three studied Hycleus species. Comparative analyses of two IGSs suggest that their evolutionary mechanisms are duplication/random loss model and slipped-strand mispairing

Olfactory Ensheathing Cells Alleviate Facial Pain in Rats with Trigeminal Neuralgia by Inhibiting the Expression of P2X7 Receptor

Trigeminal neuralgia (TN) is a common facial neuropathic pain that is mainly characterized by spontaneous or induced needling or electric shock pain in the innervation area of the trigeminal nerve. It is also referred to as “the cancer that never dies”. The olfactory ensheathing cell (OEC) is a special glial cell in the nervous system that has a strong supportive function in nerve regeneration. Cell transplantation therapy is a useful treatment modality that we believe can be applied in TN management. In this study, OECs were transplanted into the ligation site of the infraorbital nerve of rats. We found that after the OEC transplantation, mechanical pain threshold in the face of the rats was significantly increased. Western blotting, immunofluorescence assay, and reverse transcription-quantitative polymerase chain reaction were performed on the trigeminal ganglia (TG) of model rats. The results revealed a decrease in the expression of P2X7 receptor (P2X7R) in the trigeminal ganglia. Our findings show that OEC transplantation has a good therapeutic effect on TN in rats, and that can reduce the expression of P2X7R in trigeminal ganglia. Therefore, we think that OEC transplantation may be a suitable treatment for TN

Additional file 4: Table S4. of Mitochondrial genomes of blister beetles (Coleoptera, Meloidae) and two large intergenic spacers in Hycleus genera

Annotation of the Hycleus cichorii mitogenome. (DOCX 22 kb

Additional file 12: Table S11. of Mitochondrial genomes of blister beetles (Coleoptera, Meloidae) and two large intergenic spacers in Hycleus genera

The best-fit schemes and evolutionary models for two datasets. (DOCX 16 kb

Additional file 2: Table S2. of Mitochondrial genomes of blister beetles (Coleoptera, Meloidae) and two large intergenic spacers in Hycleus genera

Annotation of the Hycleus phaleratus mitogenome. (DOCX 21 kb

Design Rules of the Mixing Phase and Impacts on Device Performance in High-Efficiency Organic Photovoltaics

In nonfullerene acceptor- (NFA-) based solar cells, the exciton splitting takes place at both domain interface and donor/acceptor mixture, which brings in the state of mixing phase into focus. The energetics and morphology are key parameters dictating the charge generation, diffusion, and recombination. It is revealed that tailoringthe electronic properties of the mixing region by doping with larger-bandgap components could reduce the density of state but elevate the filling state level, leading to improved open-circuit voltage (VOC) and reduced recombination. The monomolecular and bimolecular recombinations are shown to be intercorrelated, which show a Gaussian-like relationship with VOC and linear relationship with short-circuit current density (JSC) and fill factor (FF). The kinetics of hole transfer and exciton diffusion scale with JSC similarly, indicating the carrier generation in mixing region and crystalline domain are equally important. From the morphology perspective, the crystalline order could contribute to VOC improvement, and the fibrillar structure strongly affects the FF. These observations highlight the importance of the mixing region and its connection with crystalline domains and point out the design rules to optimize the mixing phase structure, which is an effective approach to further improve device performance