Additional file 1: of Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato

Figure S1. Purification and necrosis activity of the protein BcGs1. A: SDS-PAGE analysis of purified BcGs1. M, Protein marker. 1, Purified BcGs1. B: Necrosis activity of BcGs1 in tomato, tobacco, cucumber and pea leaves. (DOCX 673 kb

Unexpected C–C Bond Cleavage: A Route to 3,6-Diarylpyridazines and 6‑Arylpyridazin-3-ones from 1,3-Dicarbonyl Compounds and Methyl Ketones

An unexpected C–C bond cleavage has been revealed in the absence of metal. This observation has been exploited to develop an efficient approach toward 3,6-diarylpyridazines and 6-arylpyridazin-3-ones from simple and commercially available 1,3-dicarbonyl compounds and methyl ketones

Unexpected C–C Bond Cleavage: A Route to 3,6-Diarylpyridazines and 6‑Arylpyridazin-3-ones from 1,3-Dicarbonyl Compounds and Methyl Ketones

An unexpected C–C bond cleavage has been revealed in the absence of metal. This observation has been exploited to develop an efficient approach toward 3,6-diarylpyridazines and 6-arylpyridazin-3-ones from simple and commercially available 1,3-dicarbonyl compounds and methyl ketones

Unexpected C–C Bond Cleavage: A Route to 3,6-Diarylpyridazines and 6‑Arylpyridazin-3-ones from 1,3-Dicarbonyl Compounds and Methyl Ketones

An unexpected C–C bond cleavage has been revealed in the absence of metal. This observation has been exploited to develop an efficient approach toward 3,6-diarylpyridazines and 6-arylpyridazin-3-ones from simple and commercially available 1,3-dicarbonyl compounds and methyl ketones

Table_4_Is there a causal association between gestational diabetes mellitus and immune mediators? A bidirectional Mendelian randomization analysis.xlsx

BackgroundDiabetes that only appears or is diagnosed during pregnancy is referred to as gestational diabetes mellitus (GDM). The maternal physiological immune profile is essential for a positive pregnancy outcome. However, the causal relationship between GDM and immunophenotypes is not fully defined.MethodsBased on the high-density genetic variation data at the genome-wide level, we evaluated the logical associations between 731 specific immune mediators and GDM using bidirectional Mendelian randomization (MR). The inverse variance weighted (IVW) was the main method employed for MR analysis. We performed multiple methods to verify the robustness and dependability of the MR results, and sensitivity measures were applied to rule out potential heterogeneity and horizontal pleiotropy.ResultsA substantial causal association between several immune mediators and GDM was detected. After FDR testing, HLA DR++ monocyte %leukocyte and HLA DR on plasmacytoid DC were shown to increase the risk of GDM; in contrast, CD127 on CD28+ CD45RA+ CD8br and CD19 on PB/PC were shown to attenuate the effect of GDM. Moreover, the progression of GDM has been shown to decrease the maternal levels of CD39+ activated Treg AC, CD39+ activated Treg �4 Treg, CD39+ resting Treg AC, CD39+ resting Treg �4 Treg, and CD39+ CD8BR %T cell.ConclusionsOur findings support a possible causal association between GDM and various immunophenotypes, thus facilitating the provision of multiple options for preventive recognition as well as for the diagnostic and therapeutic management of GDM in clinical practice.</p

Secondary clustering of LIHC patients in TCGA based on the expression of NCLs.

(A) Tumor samples were divided into four subgroups. (B) Correlation plots between the four subgroups and the high & low risk categories. (C) Survival curves for four subgroups. (D-G) PCA and t-SNE analyses of four subgroups. (H-J) Immune cell infiltration in four subgroups.</p

Enrichment analyses.

GO (A, B) and KEGG (C) enrichment, along with multiple GSEA analyses (D, E).</p

Modelling to determine the prognostic value of NCLs in LIHC.

(A) Co-expression analysis of NETosis with lncRNAs. (B) Forest plot findings from univariate Cox regression analysis. (C, D) Lasso analysis and Cox regression for the construction of models. (E) Correlation analysis of lncRNAs with NETosis genes.</p

Image_4_Is there a causal association between gestational diabetes mellitus and immune mediators? A bidirectional Mendelian randomization analysis.tif

BackgroundDiabetes that only appears or is diagnosed during pregnancy is referred to as gestational diabetes mellitus (GDM). The maternal physiological immune profile is essential for a positive pregnancy outcome. However, the causal relationship between GDM and immunophenotypes is not fully defined.MethodsBased on the high-density genetic variation data at the genome-wide level, we evaluated the logical associations between 731 specific immune mediators and GDM using bidirectional Mendelian randomization (MR). The inverse variance weighted (IVW) was the main method employed for MR analysis. We performed multiple methods to verify the robustness and dependability of the MR results, and sensitivity measures were applied to rule out potential heterogeneity and horizontal pleiotropy.ResultsA substantial causal association between several immune mediators and GDM was detected. After FDR testing, HLA DR++ monocyte %leukocyte and HLA DR on plasmacytoid DC were shown to increase the risk of GDM; in contrast, CD127 on CD28+ CD45RA+ CD8br and CD19 on PB/PC were shown to attenuate the effect of GDM. Moreover, the progression of GDM has been shown to decrease the maternal levels of CD39+ activated Treg AC, CD39+ activated Treg �4 Treg, CD39+ resting Treg AC, CD39+ resting Treg �4 Treg, and CD39+ CD8BR %T cell.ConclusionsOur findings support a possible causal association between GDM and various immunophenotypes, thus facilitating the provision of multiple options for preventive recognition as well as for the diagnostic and therapeutic management of GDM in clinical practice.</p

Table_2_Is there a causal association between gestational diabetes mellitus and immune mediators? A bidirectional Mendelian randomization analysis.xlsx

BackgroundDiabetes that only appears or is diagnosed during pregnancy is referred to as gestational diabetes mellitus (GDM). The maternal physiological immune profile is essential for a positive pregnancy outcome. However, the causal relationship between GDM and immunophenotypes is not fully defined.MethodsBased on the high-density genetic variation data at the genome-wide level, we evaluated the logical associations between 731 specific immune mediators and GDM using bidirectional Mendelian randomization (MR). The inverse variance weighted (IVW) was the main method employed for MR analysis. We performed multiple methods to verify the robustness and dependability of the MR results, and sensitivity measures were applied to rule out potential heterogeneity and horizontal pleiotropy.ResultsA substantial causal association between several immune mediators and GDM was detected. After FDR testing, HLA DR++ monocyte %leukocyte and HLA DR on plasmacytoid DC were shown to increase the risk of GDM; in contrast, CD127 on CD28+ CD45RA+ CD8br and CD19 on PB/PC were shown to attenuate the effect of GDM. Moreover, the progression of GDM has been shown to decrease the maternal levels of CD39+ activated Treg AC, CD39+ activated Treg �4 Treg, CD39+ resting Treg AC, CD39+ resting Treg �4 Treg, and CD39+ CD8BR %T cell.ConclusionsOur findings support a possible causal association between GDM and various immunophenotypes, thus facilitating the provision of multiple options for preventive recognition as well as for the diagnostic and therapeutic management of GDM in clinical practice.</p