JH and insulin regulate starvation resistance.

<p>A. RNAi-aided knockdown in the expression of genes coding for JHAMT or Met extended the starvation survival. Percent beetles survived during starvation after injection of control <i>malE</i>, JHAMT, or Met dsRNA respectively into 40 newly emerged male adults are shown. Starvation survival was recorded from day 4 to day 14. B. RNAi-aided knockdown in the expression of genes coding for ILP2 extend starvation survival. Percent beetles survived during starvation after injection of control <i>malE</i>, ILP1, ILP2, ILP3, or ILP4 dsRNA respectively into 40 new emerged male adults are shown. Starvation survival was recorded from day 4 to day 14. C. Bovine insulin can rescue the starvation survival of JHAMT or ILP2 RNAi beetles. Percent beetles survived after injection of control <i>malE</i>, JHAMT, ILP2 dsRNA into day 0 male adults followed by injection of either 25 mM HEPES or 25 mM HEPES containing 10 mg/ml insulin into day 5 adults are shown. The starvation survival was recorded from day 9 to day 14. D. JH III rescues starvation survival in JHAMT but not in ILP2 RNAi beetles. Shown are percentages of beetles survived after injection of control <i>malE</i>, JHAMT, ILP2 dsRNA into day 0 male adults followed by topical application of either acetone or 10 mM JH III in acetone on days 3, 5, and 7. The starvation survival was recorded from day 9 to day 14.</p

The relative mRNA levels of TRET and trehalase in the alimentary canal, fat body, and head after manipulation of JH or insulin levels.

<p>A. The relative mRNA levels of TRET in the alimentary canal, fat body, and head after injection of <i>malE</i>, JHAMT, ILP2, or Met dsRNA into newly emerged male adults. Total RNA was isolated and used to measure relative TRET mRNA by qRT-PCR using RP49 as a control. The data shown are the Mean+S.D. (n = 3). B. The relative mRNA levels of TRET in the alimentary canal, fat body, and head after topical application of 0.5 µl acetone, 0.5 µl 10 mM JH III in acetone, or injection of 0.3 µl HEPES solution or bovine insulin in HEPES solution at 10 mg/ml concentration. Total RNA was isolated from the tissues including fat body, alimentary canal, and head dissected from the starved beetles at 6 hours after treatment on day 5 for JH and insulin induction experiment. The total RNA was converted to cDNA, and the relative TRET mRNA levels were determined by qRT-PCR using RP49 as a control. The data shown are the Mean+S.D. (n = 3). C. Same treatments as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003535#pgen-1003535-g005" target="_blank">Figure 5A</a> except trehalose mRNA levels were determined. D. Same treatments as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003535#pgen-1003535-g005" target="_blank">Figure 5B</a> except trehalose mRNA levels were determined.</p

Juvenile Hormone and Insulin Regulate Trehalose Homeostasis in the Red Flour Beetle, <i>Tribolium castaneum</i>

<div><p>Insulin/IGF-1 signaling (IIS) has been well studied for its role in the control of life span extension and resistance to a variety of stresses. The <i>Drosophila melanogaster</i> insulin-like receptor (InR) mutant showed extended life span due to reduced juvenile hormone (JH) levels. However, little is known about the mechanism of cross talk between IIS and JH in regulation of life span extension and resistance to starvation. In the current study, we investigated the role of IIS and JH signaling in regulation of resistance to starvation. Reduction in JH biosynthesis, JH action, or insulin-like peptide 2 (ILP2) syntheses by RNA interference (RNAi)-aided knockdown in the expression of genes coding for juvenile hormone acid methyltransferase (JHAMT), methoprene-tolerant (Met), or ILP2 respectively decreased lipid and carbohydrate metabolism and extended the survival of starved beetles. Interestingly, the extension of life span could be restored by injection of bovine insulin into JHAMT RNAi beetles but not by application of JH III to ILP2 RNAi beetles. These data suggest that JH controls starvation resistance by regulating synthesis of ILP2. More importantly, JH regulates trehalose homeostasis, including trehalose transport and metabolism, and controls utilization of stored nutrients in starved adults.</p></div

JH and ILP2 regulate carbohydrate, protein, and lipid metabolism during starvation.

<p>A. Total carbohydrate, protein, and lipid levels were determined by Anthrone reagent, Bradford, and vanillin reagent respectively in samples collected from day 3 to day 8 starved and fed male beetles. Three beetles were used for each time point and six biological replicate were used. The Means+S.D (n = 6) are shown. B. The nutrient levels including carbohydrate, protein, and lipid are regulated by JH and ILP2 during starvation. The male beetles injected with <i>malE</i>, ILP2, or JHAMT dsRNA were collected on day 8. The total carbohydrate, protein, and lipid were determined. Shown are the Means+S.D (n = 6). Asterisks show treatments that are significantly different (<i>P</i><0.05) by one-way ANOVA.</p

Trehalose metabolism plays an important role in extending life span during starvation.

<p>A. Starvation survival after feeding 10% trehalose plus cellulose, 10% glucose plus cellulose, or cellulose alone to the 8-day-old starved male beetles. The survival was recorded from day 9 to day 12. B. The ratio between hemolymph glucose and trehalose on days 4, 5, and 6 after injection of <i>malE</i>, ILP2, JHAMT, Met, TRET, or TPS dsRNA into the newly emerged adults. The hemolymph was extracted from three beetles for each treatment. The trehalose concentrations were determined using the glucose reagent and trehalase. The data shown are the Mean+S.D. (n = 6). C. Starvation survival after manipulation of endogenous trehalose level by injecting TRET, TPS, or trehalase dsRNA on day 0. The beetles were starved until 14 days. The survival was recorded from day 9 to day 14. D. The relative mRNA level of TPS, TRET, and trehalase after injecting <i>malE</i>, JHAMT, or ILP2 dsRNA. Total RNA was isolated on day 5 from beetles injected with control <i>malE</i>, JHAMT, or ILP2 dsRNA and starved. The RNA was converted to cDNA, and the relative levels of TPS, TRET, and trehalase mRNA were determined by qRT-PCR using RP49 as a control. The data shown are the Mean+S.D. (n = 3). Asterisks show treatments that are significantly different from the control (<i>P</i><0.05) in one-way ANOVA.</p

The relative mRNA levels of TRET, TPS, and trehalase in the fat body, head, male accessory gland, testis, and alimentary canal.

<p>A. Total RNA was isolated from the tissues dissected from the fed beetles from day 1 to day 4, the total RNA was converted to cDNA, and the relative TPS, TRET, and trehalase mRNA levels were determined by qRT-PCR using RP49 as a control. The data shown are the Mean+S.D. (n = 3). Asterisks show treatments that are significantly different from the control (<i>P</i><0.05) in one-way ANOVA. B. The relative mRNA level of TRET, TPS, and trehalase in the starved and fed male beetles collected on day 1 to day 5 after adult emergence. Total RNA was isolated from the whole body of both fed and starved beetles on days 1 to 5. The total RNA was converted to cDNA, and the relative TPS, TRET, and trehalase mRNA levels were determined by qRT-PCR using RP49 as a control. The data shown are the Mean+S.D. (n = 3).</p

Core–Shell Molecularly Imprinted Polymer Nanoparticles as Synthetic Antibodies in a Sandwich Fluoroimmunoassay for Trypsin Determination in Human Serum

We describe the application of a fluorescently labeled water-soluble core–shell molecularly imprinted polymer (MIP) for fluorescence immunoassay (FIA) to detect trypsin. <i>p</i>-Aminobenzamidine (PAB), a competitive inhibitor of trypsin, was immobilized in the wells of a microtiter plate enabling the capture of trypsin in an oriented position, thus maintaining its native conformation. Fluorescent MIP nanoparticles, which bound selectively to trypsin, were used for quantification. The MIP was prepared by a multistep solid-phase synthesis approach on glass beads functionalized with PAB, orientating all trypsin molecules in the same way. The core–MIP was first synthesized, using a thermoresponsive polymer based on <i>N</i>-isopropylacrylamide, so as to enable its facile liberation from the immobilized template by a simple temperature change. The shell, mainly composed of allylamine to introduce primary amino groups for postconjugation of fluorescein isothiocyanate (FITC), was grafted in situ on the core–MIP, whose binding cavities were still bound and protected by the immobilized trypsin. The resulting core–shell MIP was endowed with a homogeneous population of high-affinity binding sites, all having the same orientation. The MIP has no or little cross-reactivity with other serine proteases and unrelated proteins. Our MIP-based FIA system was successfully applied to detect low trypsin concentrations spiked into nondiluted human serum with a low limit of quantification of 50 pM, which indicates the significant potential of this assay for analytical and biomedical diagnosis applications

Additional file 1: Figure S1. of Performance of Nano-Submicron-Stripe Pd Thin-Film Temperature Sensors

The red lines are the isothermal lines for the expected temperature distribution on the substrate surface. The sensors in the testing array are numbered the same series numbers from 1 to 9 as that in the main text (DOC 363 kb)

Desarrollo de un programa para optimizar el grado de servicio de las centrales digitales de la Ciudad de Guayaquil para IETEL R-2

Presenta un programa para calcular el grado de servicios y obtener una mejor calidad de servicio de las centrales telefónicas. Hace una breve descripción de las centrales telefónicas analógicas y digitales y de la medición de tráfico y estudio de rutas. Planifica la adaptación de la central digital a un computador personal y por último presenta el programa con sus algoritmos y ejemplos.GuayaquilIngeniero en Electricidad Especialización Electrónic

A novel adaptive terahertz system for reliable and efficient maritime communications under hostile sea conditions

Terahertz (THz) frequency band has been widely used in indoor, outdoor and space communications due to its advantage of large available bandwidth. However, limited research has been conducted to apply THz technique in maritime communications as hostile sea conditions cause significant path loss, thus leading to unacceptable signal error rate. In this paper, we propose a novel adaptive THz maritime communication system to tackle the above-mentioned challenge. Specifically, we design a joint source-channel coding scheme by using system random linear network coding (sRLNC) and Reed-Solomon (RS) to ensure transmission reliability. To further improve the transmission efficiency, we propose a novel triple-channel communication architecture facilitated by a very high frequency (VHF) feedback channel. With this design, the source data can be transmitted via the THz main channel while the coding redundancy is dispatched in the auxiliary channel. Meanwhile, the feedback channel allows sender to use an adaptive mechanism to achieve the transmission efficiency with higher transmission rate over long communication distance. In addition, we adopt the Doppler frequency offset in maritime environment to compensate both relative movement between communication parties and adversarial maritime factors, e.g., strong wind and extreme sea states. Simulation results demonstrate that our proposed THz system has remarkable capability not only to improve the communication efficiency up to 20Gbps compared to those conventional high frequency (HF), VHF and millimeter wave communication systems but also to transmit data over a longer distance with lower BERs.</p