Clustering analysis of TS dependent cytotoxicity in HCT-C18 (TS+) cells (Lane 1) treated with 10 μM 5-FU for 4 hrs (Lane 2) and 24 hrs (Lane 3) based on the expression profiles generated from steady state total mRNAs via One-way ANOVA analysis with p 0

<p><b>Copyright information:</b></p><p>Taken from "Multi-level gene expression profiles affected by thymidylate synthase and 5-fluorouracil in colon cancer"</p><p>BMC Genomics 2006;7():68-68.</p><p>Published online 3 Apr 2006</p><p>PMCID:PMC1448211.</p><p>Copyright © 2006 Xi et al; licensee BioMed Central Ltd.</p>05

Correlation between miR-205 and PTEN expression in endometrial cancer patients.

<p>(A) Correlation of miR-205 and PTEN expression was analyzed by two-tailed Spearman nonparametric correlation test (P = 0.034, Spearman correlation coefficient = −0.502). PTEN protein expression accompanied by miR-205 expression (in triplicate) is illustrated in individual cases; undifferentiated carcinoma (B), and clear cell carcinoma (C).</p

Sequences of PCR primers and probes used in qRT-PCR of PTEN mRNA.

<p>Sequences of PCR primers and probes used in qRT-PCR of PTEN mRNA.</p

miRNA expression in normal and endometrial cancer tissue specimens.

<p>Relative quantification of miRNAs was expressed as normalized with an internal control RNU6B gene. (A) miR-26a expression (P = 0.742). (B) let-7g expression (p = 0.91). (C) miR-21 expression (P = 0.641). (D) miR-181b expression (P = 0.313). (E) miR-192 expression (P = 0.106). (F) miR-215 expression (P = 0.336). (G) miR-200c expression (P<0.0001). (H) miR-205 expression (P<0.0001). Statistical significance was calculated by a paired Student's t-test.</p

Characteristics of endometrial cancer patients used for the study.

<p>Characteristics of endometrial cancer patients used for the study.</p

Clustering analysis of predictive marker genes for TS independent cytotoxicity in HCT-C18 (TS-) cells (Lane 1) treated with 10 μM 5-FU for 4 hrs (Lane 2) and 24 hrs (Lane 3) via One-way ANOVA analysis with p 0

<p><b>Copyright information:</b></p><p>Taken from "Multi-level gene expression profiles affected by thymidylate synthase and 5-fluorouracil in colon cancer"</p><p>BMC Genomics 2006;7():68-68.</p><p>Published online 3 Apr 2006</p><p>PMCID:PMC1448211.</p><p>Copyright © 2006 Xi et al; licensee BioMed Central Ltd.</p>05

Clinical and pathologic parameters of gastric cancer patients treated with Doxifluridine/Oxaliplatin or S-1/Oxaliplatin.

<p>Clinical and pathologic parameters of gastric cancer patients treated with Doxifluridine/Oxaliplatin or S-1/Oxaliplatin.</p

Expression levels of miR-140, miR-192, miR-200c, let-7g in both normal and gastric cancer specimens.

<p>Expression levels of miR-140, miR-192, miR-200c, let-7g in both normal and gastric cancer specimens.</p

Hypoxia induces intracellular MT1-MMP trafficking to the cell surface, resulting in enhanced invasiveness of SK-3rd cells.

<p>A) Determination of MT1-MMP expression in SK-3rd cells: Total cell lysates from SK-3rd cells either overexpressing of MT1-GFP or silencing of endogenous MT1-MMP were analyzed by Western blotting using anti-MT1-MMP antibodies. β-Actin was used as a loading control. B) Examination of the role of MT1-MMP in SK-3rd TIC invasion: SK-3rd TICs with overexpression or downregulation of MT1-MMP as indicated were pretreated with or without CoCl₂ (250 µM) for 24 hours followed by evaluation of cell invasive ability in the 3-D invasion assay (Left panel). The invaded cells were microscopically counted (Right panel). Hypoxia promotes SK-3rd TIC invasion and this process requires expression of MT1-MMP in the cells. C) Expression of MT1-MMP in TICs: Total RNAs from breast cancer cells (SK-BR3 parental cells and SK-3rd TICs) and colon cancer cells (HCT116 parental cells and HCT116 TICs) were examined for the expression of MT1-MMP using a real time RT-PCR approach. Housekeeping genes (GAPDH and HPRT) were used to normalize MT1-MMP mRNA in all samples. D) Determination of functional MT1-MMP in SK-3rd TICs: Top panel: Cell surface MT1-MMP in SK-BR3 and SK-3rd cells treated with or without CoCl₂ for 24 hours were biotinylated and precipitated with streptavidin-beads followed by Western blotting using anti-MT1-MMP antibodies. Densitometry analysis was performed and ratios between MT1-MMP latent and active forms over β-actin were given. Middle panel: An aliquot of total cell lysates of cell surface biotinylated SK-BR3 and SK-3rd cells were examined by Western blotting using anti-MT1-MMP antibodies. β-actin was used as a loading control. Densitometry analysis was performed and ratios between MT1-MMP latent and active forms over β-actin were given. Bottom panel: The conditioned medium in the presence of recombinant proMMP-2 was examined by gelatin zymogram to determine functional MT1-MMP. E) Effect of CoCl₂ on MT1-MMP expression: SK-BR3 and SK-3rd cells treated with or without CoCl₂ (250 µM) for 24 hours were examined by real time RT-PCR using MT1-MMP specific primers and housekeeping genes for normalization. F–G) Correlation of cell surface expression of MT1-MMP with proMMP-2 activation: SK-3rd TICs pretreated with CoCl₂ (Pre CoCl₂; 250 µM) for 24 hours were switched to complete medium in the absence of CoCl₂ (Post CoCl₂) for indicated dates followed by flow cytometry analysis using anti-MT1-MMP antibody (F). The cells were also examined by cell surface biotinylation experiment using antibodies for MT1-MMP (G, Upper panel) and β-actin (G, loading control, Middle panel), and gelatin zymogram (G, Lower panel) and input of MT1-MMP (Bottom panel). Densitometry analysis was performed and ratios between MT1-MMP active form over β-actin was given.</p

Kaplan-Meier overall survival curves of patients treated with S-1/Oxaliplatin and Doxifluridine/Oxaliplatin in association with miR-21 (A) and miR-181b (B).

<p>Kaplan-Meier overall survival curves of patients treated with S-1/Oxaliplatin and Doxifluridine/Oxaliplatin in association with miR-21 (A) and miR-181b (B).</p