Litter Decomposition in a Semiarid Dune Grassland: Neutral Effect of Water Supply and Inhibitory Effect of Nitrogen Addition.

BACKGROUND:The decomposition of plant material in arid ecosystems is considered to be substantially controlled by water and N availability. The responses of litter decomposition to external N and water, however, remain controversial, and the interactive effects of supplementary N and water also have been largely unexamined. METHODOLOGY/PRINCIPAL FINDINGS:A 3.5-year field experiment with supplementary nitrogen and water was conducted to assess the effects of N and water addition on mass loss and nitrogen release in leaves and fine roots of three dominant plant species (i.e., Artemisia halondendron, Setaria viridis, and Phragmites australis) with contrasting substrate chemistry (e.g. N concentration, lignin content in this study) in a desertified dune grassland of Inner Mongolia, China. The treatments included N addition, water addition, combination of N and water, and an untreated control. The decomposition rate in both leaves and roots was related to the initial litter N and lignin concentrations of the three species. However, litter quality did not explain the slower mass loss in roots than in leaves in the present study, and thus warrant further research. Nitrogen addition, either alone or in combination with water, significantly inhibited dry mass loss and N release in the leaves and roots of the three species, whereas water input had little effect on the decomposition of leaf litter and fine roots, suggesting that there was no interactive effect of supplementary N and water on litter decomposition in this system. Furthermore, our results clearly indicate that the inhibitory effects of external N on dry mass loss and nitrogen release are relatively strong in high-lignin litter compared with low-lignin litter. CONCLUSION/SIGNIFICANCE:These findings suggest that increasing precipitation hardly facilitates ecosystem carbon turnover but atmospheric N deposition can enhance carbon sequestration and nitrogen retention in desertified dune grasslands of northern China. Additionally, litter quality of plant species should be considered when modelling the carbon cycle and nutrient dynamics of this system

Third-Generation Sequencing and Analysis of Four Complete Pig Liver Esterase Gene Sequences in Clones Identified by Screening BAC Library.

AIM:Pig liver carboxylesterase (PLE) gene sequences in GenBank are incomplete, which has led to difficulties in studying the genetic structure and regulation mechanisms of gene expression of PLE family genes. The aim of this study was to obtain and analysis of complete gene sequences of PLE family by screening from a Rongchang pig BAC library and third-generation PacBio gene sequencing. METHODS:After a number of existing incomplete PLE isoform gene sequences were analysed, primers were designed based on conserved regions in PLE exons, and the whole pig genome used as a template for Polymerase chain reaction (PCR) amplification. Specific primers were then selected based on the PCR amplification results. A three-step PCR screening method was used to identify PLE-positive clones by screening a Rongchang pig BAC library and PacBio third-generation sequencing was performed. BLAST comparisons and other bioinformatics methods were applied for sequence analysis. RESULTS:Five PLE-positive BAC clones, designated BAC-10, BAC-70, BAC-75, BAC-119 and BAC-206, were identified. Sequence analysis yielded the complete sequences of four PLE genes, PLE1, PLE-B9, PLE-C4, and PLE-G2. Complete PLE gene sequences were defined as those containing regulatory sequences, exons, and introns. It was found that, not only did the PLE exon sequences of the four genes show a high degree of homology, but also that the intron sequences were highly similar. Additionally, the regulatory region of the genes contained two 720bps reverse complement sequences that may have an important function in the regulation of PLE gene expression. SIGNIFICANCE:This is the first report to confirm the complete sequences of four PLE genes. In addition, the study demonstrates that each PLE isoform is encoded by a single gene and that the various genes exhibit a high degree of sequence homology, suggesting that the PLE family evolved from a single ancestral gene. Obtaining the complete sequences of these PLE genes provides the necessary foundation for investigation of the genetic structure, function, and regulatory mechanisms of the PLE gene family

Nitrogen remaining (% of initial) vs. dry mass remaining for all replicates, harvests, and treatments in leaves and roots of the three studied species.

<p>The dashed line indicates the initial N value of 100%. The abbreviations are as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0162663#pone.0162663.g002" target="_blank">Fig 2</a>.</p

Pore-Scale Simulations of CO2/Oil Flow Behavior in Heterogeneous Porous Media under Various Conditions

Miscible and near-miscible flooding are used to improve the performance of carbon-dioxide-enhanced oil recovery in heterogeneous porous media. However, knowledge of the effects of heterogeneous pore structure on CO2/oil flow behavior under these two flooding conditions is insufficient. In this study, we construct pore-scale CO2/oil flooding models for various flooding methods and comparatively analyze CO2/oil flow behavior and oil recovery efficiency in heterogeneous porous media. The simulation results indicate that compared to immiscible flooding, near-miscible flooding can increase the CO2 sweep area to some extent, but it is still inefficient to displace oil in small pore throats. For miscible flooding, although CO2 still preferentially displaces oil through big throats, it may subsequently invade small pore throats. In order to substantially increase oil recovery efficiency, miscible flooding is the priority choice; however, the increase of CO2 diffusivity has little effect on oil recovery enhancement. For immiscible and near-miscible flooding, CO2 injection velocity needs to be optimized. High CO2 injection velocity can speed up the oil recovery process while maintaining equivalent oil recovery efficiency for immiscible flooding, and low CO2 injection velocity may be beneficial to further enhancing oil recovery efficiency under near-miscible conditions

Dry mass remaining and decomposition rates in leaves and roots of the three species in the four treatments: N addition, water addition, combined addition of N and water, and control.

<p>The values are means±SE (<i>n</i> = 5). The results of two-way ANOVA (N addition and water addition as main effects) are shown on each sampling date for each litter type of the three species. Significant effects of nitrogen addition and water addition on dry mass loss is indicated by * and #, respectively, at each sampling time. * or # (<i>p</i><0.05); **(<i>p</i><0.01); ***(<i>p</i><0.001). The abbreviations are as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0162663#pone.0162663.g002" target="_blank">Fig 2</a>.</p

Initial leaf and root chemistry of the three dominant plant species in a desertified dune grassland in Northern China.

<p>Initial leaf and root chemistry of the three dominant plant species in a desertified dune grassland in Northern China.</p

Pore-Scale Simulations of CO₂/Oil Flow Behavior in Heterogeneous Porous Media under Various Conditions

Miscible and near-miscible flooding are used to improve the performance of carbon-dioxide-enhanced oil recovery in heterogeneous porous media. However, knowledge of the effects of heterogeneous pore structure on CO2/oil flow behavior under these two flooding conditions is insufficient. In this study, we construct pore-scale CO2/oil flooding models for various flooding methods and comparatively analyze CO2/oil flow behavior and oil recovery efficiency in heterogeneous porous media. The simulation results indicate that compared to immiscible flooding, near-miscible flooding can increase the CO2 sweep area to some extent, but it is still inefficient to displace oil in small pore throats. For miscible flooding, although CO2 still preferentially displaces oil through big throats, it may subsequently invade small pore throats. In order to substantially increase oil recovery efficiency, miscible flooding is the priority choice; however, the increase of CO2 diffusivity has little effect on oil recovery enhancement. For immiscible and near-miscible flooding, CO2 injection velocity needs to be optimized. High CO2 injection velocity can speed up the oil recovery process while maintaining equivalent oil recovery efficiency for immiscible flooding, and low CO2 injection velocity may be beneficial to further enhancing oil recovery efficiency under near-miscible conditions

Microclimatic conditions during the experimental period from 2010 to 2013 at the study site: natural rainfall, artificial rainfall (water addition) and mean temperature.

<p>Microclimatic conditions during the experimental period from 2010 to 2013 at the study site: natural rainfall, artificial rainfall (water addition) and mean temperature.</p

The gene sequence map of vector pCC1BAC.

<p>The gene sequence map of vector pCC1BAC.</p