1 research outputs found
Thermoresponsive Self-Assembled β‑Cyclodextrin-Modified Surface for Blood Purification
For patients with liver failure,
bilirubin (BR) is one of the endogenous toxins in their blood. Although
blood purification can remove the bilirubin from the body in clinics,
the detoxification system needs to be improved, and the cost needs
to be decreased. In the present study, we developed a recyclable model
surface that can strongly remove bilirubin. We first prepared <i>adamantane</i> (<i>Ad</i>) on a model gold surface
by self-assembly. Then, we integrated the <i>β-cyclodextrin
dimer</i> (<i>CDD</i>) onto the surface with host–guest
interactions between one of the CD cavities in the <i>CDD</i> and <i>Ad</i>. We characterized the surface with XPS,
static contact angle measurements, and AFM. In addition, we employed
QCM-D to characterize the preparation process as well as the detoxification
of the surface. We demonstrated that this modified surface could strongly
adsorb bilirubin through host–guest interactions between the
CD cavities in the <i>CDD</i> and bilirubin and that the
detoxification was improved 1.7 times (compared to the surface only
with <i>Ad</i>). Interestingly, after characterization with
QCM-D, this surface could be recycled due to the thermoresponsive
property of the host–guest interaction between the <i>CDD</i> and <i>Ad</i>. After adsorbing the toxin and
increasing the temperature to 45 °C, the <i>CDD</i> with bilirubin could be removed from the surface. Then, the refreshed
surface with <i>CDD</i> could be prepared again at room
temperature. This cycle could be repeated at least 3 times. Additionally,
during each cycle, the modified surface exhibited good detoxification
to bilirubin. This modified surface also showed strong resistance
to plasma proteins, decreasing the adsorption of human serum albumin
(HSA) and fibrinogen (Fg). An in vitro platelet adhesion assay showed
that the adhesion of the platelets on the modified surface decreased
and that the platelets were in an inactivated state. The hemolysis
assay showed that this surface exhibited no hemolysis activity in
the samples to red blood cells (RBCs). The CCK-8 assay showed that
this surface had negligible cytotoxicity to L929 cells. This work
has taken advantage of the host–guest self-assembly between
β-CD and BR/Ad for special recognizing adsorption, as well as
the thermoresponse of β-CD-Ad inclusion for recyclable application,
and these results demonstrate that this technology has great potential
for removing bilirubin and decreasing clinic costs