DC-101: How Adaptive Aggregate Contextual Recommender Benefits IP-based TV Services

<p>DC-101: How Adaptive Aggregate Contextual Recommender Benefits IP-based TV Services</p

Video_1_Case report: Varicella-zoster virus infection triggering progressive encephalomyelitis with rigidity and myoclonus.MP4

Progressive encephalomyelitis with rigidity and myoclonus (PERM) is a rare neurological disease of unknown etiology, and most patients with PERM are positive for anti-glycine receptor (GlyR) antibody. In this case study, we report a clinical case of a varicella-zoster virus-infected patient who developed anti-GlyR antibody-positive PERM. He initially suffered from herpes zoster and gradually developed symptoms of impaired brainstem functions including hoarse voice and dysphagia, accompanied by paroxysmal sympathetic hyperactivity. The patient also suffered from severe spasms, which were easily triggered by external stimuli. Glycine receptor antibodies were then found to be positive in serum and cerebrospinal fluid, and the diagnosis of PERM was confirmed. Methylprednisolone and gamma globulin treatments were given, and spasms were improved after treatment. Unfortunately, the patient's family insisted on automatic discharge and the patient passed away several days later.</p

Primers used to make constructs to analyse promoter activity in the study.

<p>C2-A, C2-B, C2-C, C2-D, C2-E, C2-a, C2-b, C2-c and C2-d: Forward primers for construction of the luciferase reporter gene vectors pGL₃-A, pGL₃-B, pGL₃-C, pGL₃-D, pGL₃-E, pGL₃-a, pGL₃-b, pGL₃-c and pGL₃-d, respectively; C2-R: Reverse primer for construction of luciferase reporter gene vectors pGL₃-A, pGL₃-B, pGL₃-C, pGL₃-D, pGL₃-E, pGL₃-a, pGL₃-b, pGL₃-c and pGL₃-d.</p

Binding activities of MyoD and Sp1 in nuclear extracts.

<p>(A) Assay of binding activity of MyoD in nuclear extracts; (B) Assay of binding activity of Sp1 in nuclear extracts. Lanes 1, 6: nuclear extracts from C2C12 myoblasts; lanes 2, 3, 4, 7, 8, 9: nuclear extracts from C2C12 myotubes after a 4 day induction with horse serum; lanes 5, 11: competition for binding by the unlabeled probe; lane 10: positive control.</p

The expression of the <i>CRABP2</i> gene during differentiation was assessed by quantitative real-time PCR (qRT-PCR).

<p>The values were normalized to <i>GAPDH</i> mRNA expression level and the value of day 0 was set to 1. The error bars indicate the SD (n = 3).</p

The target gene expression in C2C12 cells transfected with the <i>CRABP2</i> lentivirus vector.

<p>(CON: Blank control; NC: Negative control; OE: Overexpression).</p

The second deletion analysis of the promoter of the mouse <i>CRABP2</i> gene in C2C12 cells.

<p>C2C12 cells were co-transfected with various promoter regions fused to firefly luciferase and a Renilla luciferase expression control vector. The resulting firefly luciferase activity was then normalized to Renilla luciferase activity, and the relative values were presented as the fold-increase over the activity of the promoter-less pGL₃-basic vector. Values represent the mean ± SD of three independent experiments.</p

The first deletion analysis of the promoter of the mouse <i>CRABP2</i> gene in C2C12 cells.

<p>C2C12 cells were co-transfected with various promoter regions fused to firefly luciferase and a Renilla luciferase expression control vector. The resulting firefly luciferase activity was then normalized to Renilla luciferase activity, and the relative values were presented as the fold-increase over the activity of the promoter-less pGL₃-basic vector. Values represent the mean ± SD of three independent experiments.</p

The cell morphology of 293T under fluorescence microscope (Olympus, micropublisher 3.3RTV, 100×) transfected by CRABP2 overexpression vector and the expression of CRABP2 detected by Westernblot.

<p>(A) Visible image transfected with lentivirus; (B) Green fluorescence image transfected with lentivirus; (C) CRABP2 expression by westernblot.</p

The cell morphology of C2C12 under fluorescence microscope (Olympus, micropublisher 3.3RTV, 200×) transfected by different lentivirus vector.

<p>(CON: Blank control; NC: Negative control; OE: Overexpression).</p