Statistical Literacy among Practicing Clinicians from India: A Brief Survey

Objectives: To understand statistical literacy among practicing clinicians as well as to take suggestions on its implementation in medical curriculum. Methods: A web based online survey was conducted among practising clinicians of India. The questionnaire consisted of 30 questions in three parts. Part A included questions on general information (5 questions), Part B included questions on application of statistical concepts (20 questions), and Part C asked for opinions on integration of biostatistics in medical curriculum (5 questions). Part B questions were rated on a 5-point Likert scale in which 1 indicated no confidence and 5 indicated complete confidence. Results: A total of 416 clinicians responded to the questionnaire. Complete confidence in the use of SPSS software was seen in 15.8% whereas 26.7% had no confidence in it. The highest confidence was seen in statistical equations like graphical representation of data (44.7%), and sensitivity and specificity (45.2%) whereas lowest confidence was seen in COX proportional hazard regression (12.9%) and ROC curves (11.7%). Out of 416 clinicians, 136 (32.4%) had done training in statistics at undergraduate level, 128 (30.5%) did self-learning and 152 (37.1%) had received no formal training

Process intensification for the small footprint compact heat transfer device

Process intensification for the development of compact heat exchanger with small footprint is greatest challenge of the heat exchanger technology today. In the present study, a heat transfer device, coiled flow inverter (CFI) is revamped for the better heat transfer efficiency with a smaller footprint. The proposed small foot-print coiled flow inverter (SFCFI) is fabricated by bending of helical coil at 90° with equal arm lengths before and after the bend with variable curvature radius. In integration to the improved centrifugal force due to variable curvature, the SFCFI additionally offers a complete 90 flow inversion caused by each 90 bend, which results in higher radial mixing and heat transfer. The velocity and temperature flow fields depict the improved radial mixing under the laminar flow regime for the Dean number ranges from 8 to 1581. The performance of existing CFI of same heat transfer area (0.17 m2) was studied and compared with the novel SFCFI device. The results suggest, the proposed SFCFI device provides three-fold heat transfer enhancement as compared to the straight tube of same heat transfer area at Dean number 400. Additionally, heat transfer coefficient in SFCFI enhanced by 48 % as compared to helical coil. Furthermore, SFCFI provides 18 % higher value of Nusselt number as compared to the CFI. The reason for improved heat transfer may be the enhanced centrifugal force due to additional curvature effect provided in each arm of SFCFI in the plane of vortex formation. It was interesting to note that the proposed device provides 11 % lower pressure drop as compared to the CFI. The present study may aids to the development of a novel design of compact coiled and small footprint heat transfer device.Papers presented at the 13th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Portoroz, Slovenia on 17-19 July 2017 .International centre for heat and mass transfer.American society of thermal and fluids engineers

Integrating Association Mapping, Linkage Mapping, Fine Mapping with RNA Seq Conferring Seedling Vigor Improvement for Successful Crop Establishment in Deep Sown Direct-Seeded Rice

Background: Ongoing large-scale shift towards direct seeded rice (DSR) necessitates a convergence of breeding and genetic approaches for its sustenance and harnessing natural resources and environmental benefits. Improving seedling vigour remains key objective for breeders working with DSR. The present study aims to understand the genetic control of seedling vigour in deep sown DSR. Combined genome-wide association mapping, linkage mapping, fine mapping, RNA-sequencing to identify candidate genes and validation of putative candidate genes were performed in the present study. Results: Significant phenotypic variations were observed among genotypes in both F3:4:5 and BC2F2:3 populations. The mesocotyl length showed significant positive correlation with %germination, root and shoot length. The 881 kb region on chromosome 7 reported to be associated with mesocotyl elongation. RNA-seq data and RT-PCR results identified and validated seven potential candidate genes. The four promising introgression lines free from linkage drag and with longer mesocotyl length, longer root length, semi-dwarf plant height have been identified. Conclusion: The study will provide rice breeders (1) the pre breeding material in the form of anticipated DSR adapted introgression lines possessing useful traits and alleles improving germination under deep sown DSR field conditions (2) the base for the studies involving functional characterization of candidate genes. The development and utilization of improved introgression lines and molecular markers may play an important role in genomics-assisted breeding (GAB) during the pyramiding of valuable genes providing adaptation to rice under DSR. Our results offer a robust and reliable package that can contribute towards enhancing genetic gains in direct seeded rice breeding programs

Comprehensive genome-wide identification and transferability of chromosome-specific highly variable microsatellite markers from citrus species

Abstract Citrus species among the most important and widely consumed fruit in the world due to Vitamin C, essential oil glands, and flavonoids. Highly variable simple sequence repeats (SSR) markers are one of the most informative and versatile molecular markers used in perennial tree genetic research. SSR survey of Citrus sinensis and Citrus maxima were identified perfect SSRs spanning nine chromosomes. Furthermore, we categorized all SSR motifs into three major classes based on their tract lengths. We designed and validated a class I SSRs in the C. sinensis and C. maxima genome through electronic polymerase chain reaction (ePCR) and found 83.89% in C. sinensis and 78.52% in C. maxima SSRs producing a single amplicon. Then, we selected extremely variable SSRs (> 40 nt) from the ePCR-verified class I SSRs and in silico validated across seven draft genomes of citrus, which provided us a subset of 84.74% in C. sinensis and 77.53% in C. maxima highly polymorphic SSRs. Out of these, 129 primers were validated on 24 citrus genotypes through wet-lab experiment. We found 127 (98.45%) polymorphic HvSSRs on 24 genotypes. The utility of the developed HvSSRs was demonstrated by analysing genetic diversity of 181 citrus genotypes using 17 HvSSRs spanning nine citrus chromosomes and were divided into 11 main groups through 17 HvSSRs. These chromosome-specific SSRs will serve as a powerful genomic tool used for future QTL mapping, molecular breeding, investigation of population genetic diversity, comparative mapping, and evolutionary studies among citrus and other relative genera/species