1,518 research outputs found

    Bridge’s Overall Structural Scheme Analysis in High Seismic Risk Permafrost Regions

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    The mechanism of pile-soil reaction in frozen ground is not clear at present, but it is obvious that the reduction of dead weight will be beneficial to the seismic resistance of bridges. In view of the limited bridge engineering practice in high seismic risk permafrost regions, the paper addressed the structural performance of the superstructure and its effect on piles in these special regions. Four superstructures with different dead weights were compared, and bored piles were designed. Numerical simulations were implemented to investigate the refreezing time of the bored pile foundation. The concrete pile cooled rapidly in the first two days. The refreezing times of the GFRP, prestressed concrete T-girder, integrated composite girder, and MVFT girder were 15d, 37d, 39d, and 179d, respectively. The refreezing time of a pile in the same soil layer is mainly affected by the pile’s diameter, and it is significantly correlated to the square of the pile diameter. It reflects that the selection of bridge superstructures in the permafrost region is very important, which has been ignored in previous studies. The pile length and pile diameter of the lighter superstructure can be shorter and smaller to reduce the refreezing time and alleviate the thermal disturbance. Doi: 10.28991/CEJ-2022-08-07-01 Full Text: PD

    Non-receptor cytosolic protein tyrosine kinases from various rat tissues

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    AbstractAdipocytic-cytosolic non-receptor protein tyrosine kinase (CytPTK) when activated can substitute for the insulin receptor tyrosine kinase (InsRTK), in manifesting several insulin effects in insulin-receptor independent fashion. Our aims here were to utilize PolyGlu4Tyr, a good experimental exogenous substrate for protein tyrosine kinases (PTKs) in general, for studying qualitative and quantitative parameters of CytPTKs extracted from different tissue cytosols. At the same time, we would search for a unique specific marker specifically characterizing CytPTKs. High speed supernatants of spleen, thymus, smooth muscle, lung and kidney were found to be rich in CytPTK activities. Their specific activities being 6- to 13-fold that of liver or adipose cytosols. Brain, testis and adrenal cytosols were an intermediate source of CytPTK activity, whereas CytPTK activity of heart and skeletal muscle was low. It was also evaluated that the capacity of the cytosol to phosphorylate PolyGlu4Tyr is 15–50% that of the non-stimulated Triton X-100 extractable plasma membrane PTKs. Fractionation of the cytosols on superose 12 column revealed several CytPTKs within the same tissue, their peaks ranging between 30 and 450 kDa. Immunoblotting analysis showed Fyn and Lyn were present in most tissue cytosols. Upon immunoprecipitation, however, with anti-Fyn or anti-Lyn, negligible amounts (< 2%) of the total cellular CytPTK were precipitated. Thus, these general markers of CytPTKs comprise only a minor proportion of the total intracellular PolyGlu4Tyr phosphorylating capacity. To see whether a specific marker for CytPTK could be detected, we also examined the requirement of CytPTKs for divalent ions, their preferred phosphate donor and their sensitivity to inhibition by known PTK inhibitors. We found that the order of reactivity with divalent cations was Co2+ > Mn2+ > Mg2+, while Zn2+ and Ca2+ did not support CytPTK activity. The best phosphate donor was ATP (ED50 = μM), but other nucleoside 3-phosphates could substitute for ATP at high concentrations. With respect to these parameters, no basic difference exists between cytosolic and plasma-membrane PTKs. The PTK inhibitors, genestein and quercetin, inhibited both cytosolic and membranal PTKs at micromolar concentrations. In contrast, staurosporine was a potent inhibitor of CytPTKs (IC50 5–20 nM) and a poor inhibitor of membranal PTKs (IC50 10–40 μM). One of the conclusions we can draw from this study is that tissue cytosols contain PolyGlu4Tyr phosphorylating capacity in quantities greater than previously assumed and that the low level of phosphotyrosine found in cells is not the result of limited intracellular levels of CytPTKs

    Cloud-structural health monitoring based on smartphone

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    Smartphone, integrated with CPU, sensors, network, and storage capability, is developed rapidly in recent years. A cloud-structural health monitoring method based on smartphone was proposed, and a structural health monitoring system Orion-CC, which integrates functions of data acquisition, data analysis and data upload, was developed on smartphone to perform structural health monitoring without any other professional devices. And the feasibility of Orion-CC was proved by the cable force test. A cloud-SHM data sharing website was built to make the data synchronization between smartphone and website, and realize data uploading and sharing, which can improve the efficiency of monitoring and big data integration, make the possibility for big data collection and quick structural safety evaluation
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