Purification and characterization of a cold-adapted α-amylase produced by Nocardiopsis sp. 7326 isolated from Prydz Bay, Antarctic

This is the author accepted manuscript. The final version is available from Springer via the DOI in this record. An actinomycete strain 7326 producing cold-adapted α-amylase was isolated from the deep sea sediment of Prydz Bay, Antarctic. It was identified as Nocardiopsis based on morphology, 16S rRNA gene sequence analysis, and physiological and biochemical characteristics. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zymogram activity staining of purified amylase showed a single band equal to a molecular mass of about 55 kDa. The optimal activity temperature of Nocardiopsis sp. 7326 amylase was 35°C, and the activity decreased dramatically at temperatures above 45°C. The enzyme was stable between pH 5 and 10, and exhibited a maximal activity at pH 8.0. Ca2+, Mn2+, Mg2+, Cu2+, and Co2+stimulated the activity of the enzyme significantly, and Rb2+, Hg2+, and EDTA inhibited the activity. The hydrolysates of soluble starch by the enzyme were mainly glucose, maltose, and maltotriose. This is the first report on the isolation and characterization of cold-adapted amylase from Nocardiopsis sp.National Natural Science Funds of Chin

Molecular cloning and expression of a cold-adapted lipase gene from an antarctic deep sea psychrotrophic bacterium Pseudomonas sp. 7323

This is the author accepted manuscript. The final version is available from Springer via the DOI in this record .A psychrotrophic bacterium producing a cold-adapted lipase was isolated from the deep-sea sediment of Prydz Bay, Antarctic and identified as a Pseudomonas strain. Determination of the nucleotide sequence of the gene encoding a lipase from Pseudomonas sp. 7323 (lipA) revealed that LipA is composed of 617 amino acid residues with a calculated molecular weight of 64,466 Da. LipA has a GXSXG motif, which is conserved in lipases/esterases and generally contains the active-site serine. The lipase purified from the Escherichia coli transformant (rLipA) by metal-chelating chromatography exhibited the same electrophoretic mobility as did the wild-type lipase (wLipA) purified from strain 7323, and both enzymes were quite similar in physicochemical properties. The optimal temperature and pH value for the lipases activity were 30°C and 9.0, respectively. They were unstable at temperatures above 25°C and only retained half of their highest activity after incubation at 60°C for 5 min. These results indicated that the enzymes were typical alkaline cold-adapted enzymes. Both enzymes were particularly activated by Ca2+. Additionally, the enzymes hydrolyzed p-nitrophenyl caprate and tributyrin at the highest velocity among the other p-nitrophenyl esters and triglycerides.Hi-Tech research and development program of Chin

Systematic investigation of the rotational bands in nuclei with Z≈100Z \approx 100 using a particle-number conserving method based on a cranked shell model

The rotational bands in nuclei with $Z \approx 100$ are investigated systematically by using a cranked shell model (CSM) with the pairing correlations treated by a particle-number conserving (PNC) method, in which the blocking effects are taken into account exactly. By fitting the experimental single-particle spectra in these nuclei, a new set of Nilsson parameters ($\kappa$ and $\mu$) and deformation parameters ($\varepsilon_2$ and $\varepsilon_4$) are proposed. The experimental kinematic moments of inertia for the rotational bands in even-even, odd-$A$ and odd-odd nuclei, and the bandhead energies of the 1-quasiparticle bands in odd-$A$ nuclei, are reproduced quite well by the PNC-CSM calculations. By analyzing the $\omega$-dependence of the occupation probability of each cranked Nilsson orbital near the Fermi surface and the contributions of valence orbitals in each major shell to the angular momentum alignment, the upbending mechanism in this region is understood clearly.Comment: 21 pages, 24 figures, extended version of arXiv: 1101.3607 (Phys. Rev. C83, 011304R); added refs.; added Fig. 4 and discussions; Phys. Rev. C, in pres

Local Buckling of Concrete Filled Rectangular Steel Tube with Longitudinal Stiffener under Axial Compression

Width-thickness ratio was an important parameter for designing Concrete Filled Rectangular Steel Tube (CFRST). Welding longitudinal stiffener on the internal wall of steel pipe could delay the local buckling, which increased the limit of width-thickness ratio. If there was not enough stiffener and its sectional dimension was too small, the local buckling of steel pipe would occur, inducing its bearing capacity seriously. If the stiffener sectional dimension was too large, concrete filled in steel tube would be broken up, which reduces its bearing capacity. To solve that problem, this paper studied local buckling of CFRST with longitudinal stiffener under axial compression and design of longitudinal stiffener. It established buckling analysis model, simplified local buckling analysis as calculating buckling load of thin plate clamped on loading side and unloading side under axial force. It deduced buckling load and buckling coefficient based on the principle of energy. The results showed that buckling mode depended on stiffening rigidity. Therefore, it put forward minimum stiffening rigidity ratio that controlled the stiffener design. This paper also came up with a formula to calculate minimum stiffening rigidity ratio. It provided guidance on designing number, sectional dimension and material performance