Water-Enabled Visual Detection of DNA

A water-enabled visual detection strategy has been developed for the sequence-specific identification of target DNA. The conceptual basis of the assay scheme, water condensation, is environmentally friendly and chemical transformation-free, thus offering significant assay advantages over conventional diagnostic systems. This label-free strategy operates on a target-driven generation of a hydrophilic structure and alteration of surface wettability and, consequently, transition of morphological state of and light propagation mode in the surface-condensed water. The chip array detection system, implemented herein with the ligase chain reaction-rolling circle amplification protocol, has allowed the achievement of high sensitivity (600 copies), high selectivity (single-base discrimination specificity), and multiplexed analysis capability

Cobalt(III)-Catalyzed Oxadiazole-Directed C–H Activation for the Synthesis of 1‑Aminoisoquinolines

Aromatic heterocycles have been identified as effective directing groups (DGs) in C–H functionalization but can be retained as undesired bulky substituents in the final products. Herein, we report a Co­(III)-catalyzed 1-aminoisoquinoline synthesis strategy based on oxadiazole-directed aromatic C–H coupling with alkynes and a subsequent redox-neutral C–N cyclization reaction. This labile N–O bond-based protocol has allowed the toleration of a broad range of functional groups

Dirhodium(II)-Catalyzed (3 + 2) Cycloaddition of the <i>N</i>‑Arylaminocyclopropane with Alkene Derivatives

Several (3 + 2) cycloaddition reactions catalyzed by dirhodium­(II) complexes between <i>N</i>-arylaminocyclopropane and alkenes derivative have been developed. Preliminary mechanism studies suggest that dirhodium­(II) complexes may decrease the bond-dissociation energy (BDE) of the N–H bond of <i>N</i>-arylaminocyclopropanes for N–H bond activation, thus facilitating the formation of N-centered radicals by loss of a hydrogen radical

Cobalt(III)-Catalyzed Oxadiazole-Directed C–H Activation for the Synthesis of 1‑Aminoisoquinolines

Aromatic heterocycles have been identified as effective directing groups (DGs) in C–H functionalization but can be retained as undesired bulky substituents in the final products. Herein, we report a Co­(III)-catalyzed 1-aminoisoquinoline synthesis strategy based on oxadiazole-directed aromatic C–H coupling with alkynes and a subsequent redox-neutral C–N cyclization reaction. This labile N–O bond-based protocol has allowed the toleration of a broad range of functional groups

Rhodium(III)-Catalyzed Directed <i>ortho</i>-C–H Bond Functionalization of Aromatic Ketazines via C–S and C–C Coupling

Described herein is a convenient and efficient method for sulfuration and olefination of aromatic ketazines via rhodium-catalyzed oxidative C–H bond activation. A range of substituted substrates are supported, and a possible mechanism is proposed according to experimental results of kinetic isotopic effect, reversibility studies, and catalysis of rhodacycle intermediate <b>c1</b>

Rhodium(III)-Catalyzed Oxadiazole-Directed Alkenyl C–H Activation for Synthetic Access to 2‑Acylamino and 2‑Amino Pyridines

We report herein a Rh­(III)-catalyzed alkenyl C–H activation protocol for the coupling of oxadiazoles with alkynes and synthesis of 2-acylamino and 2-amino pyridines, an important heterocyclic scaffold for various naturals products and synthetic pharmaceuticals bearing a readily reacting functional group. The selective protection/deprotection of amino groups through simple solvent switching, good functional group compatibility, superior product yield, and high regioselectivity are some of the notable synthetic features witnessed in this reaction protocol

Western-blot assay.

<p>Protein expression levels of osteogenic factors in P-MSCs treated with NPWT at different time points were shown in (A) and (B). (A) Cbfa1/Runx2 was statistically overexpressed at day 1 after treatment with NPWT, with the increase being more significant after day 3. (B) Compared with control group, the expression of OC was markedly upregulated in NPWT group after day 3. ITGB5 protein level was shown in (C), it was not affected at day 1, but was strikingly upregulated after day 3 in NPWT group. (*<i>p</i><0.05, **<i>p</i><0.01).</p

Cell apoptosis assay.

<p>Following 72 hours of treatment with suction or static conditions, apoptosis of MSCs was evaluated by TUNEL assay. The images of TUNEL⁺ cells was shown in (A, bars = 20 µm). Percentages of apoptotic cells in clots are shown in (B). Compared with control group, 72 hours of suction didn’t result in a significant increase in cell apoptosis (<i>p</i>>0.05).</p

Co(III)-Catalyzed Enaminone-Directed C–H Amidation for Quinolone Synthesis

We report herein the development of a Co­(III)-catalyzed enaminone-directed C–H amidation method for synthetic access to quinolones, an important heterocyclic scaffold for diverse pharmaceutically active structures. The C–H coupling with dioxazolones and subsequent deacylation of an installed amide group allow consecutive C–N coupling generation of quinolones with wide-ranging compatible substituent patterns

Synthesis of 2,3-Benzodiazepines via Rh(III)-Catalyzed C–H Functionalization of <i>N</i>‑Boc Hydrazones with Diazoketoesters

An efficient Rh­(III)-catalyzed C–H activation protocol has been developed for the synthesis of 2,3-benzodiazepines with use of <i>N</i>-Boc hydrazones and diazoketoesters as substrates. The reaction features retention of the CN and N–N bonds and selective cleavage of the <i>N</i>-Boc moiety