An integrated molecular cytogenetic map of Cucumis sativus L. chromosome 2

<p>Abstract</p> <p>Background</p> <p>Integration of molecular, genetic and cytological maps is still a challenge for most plant species. Recent progress in molecular and cytogenetic studies created a basis for developing integrated maps in cucumber (<it>Cucumis sativus </it>L.).</p> <p>Results</p> <p>In this study, eleven fosmid clones and three plasmids containing 45S rDNA, the centromeric satellite repeat Type III and the pericentriomeric repeat CsRP1 sequences respectively were hybridized to cucumber metaphase chromosomes to assign their cytological location on chromosome 2. Moreover, an integrated molecular cytogenetic map of cucumber chromosomes 2 was constructed by fluorescence <it>in situ </it>hybridization (FISH) mapping of 11 fosmid clones together with the cucumber centromere-specific Type III sequence on meiotic pachytene chromosomes. The cytogenetic map was fully integrated with genetic linkage map since each fosmid clone was anchored by a genetically mapped simple sequence repeat marker (SSR). The relationship between the genetic and physical distances along chromosome was analyzed.</p> <p>Conclusions</p> <p>Recombination was not evenly distributed along the physical length of chromosome 2. Suppression of recombination was found in centromeric and pericentromeric regions. Our results also indicated that the molecular markers composing the linkage map for chromosome 2 provided excellent coverage of the chromosome.</p

Binary Code Summarization: Benchmarking ChatGPT/GPT-4 and Other Large Language Models

Binary code summarization, while invaluable for understanding code semantics, is challenging due to its labor-intensive nature. This study delves into the potential of large language models (LLMs) for binary code comprehension. To this end, we present BinSum, a comprehensive benchmark and dataset of over 557K binary functions and introduce a novel method for prompt synthesis and optimization. To more accurately gauge LLM performance, we also propose a new semantic similarity metric that surpasses traditional exact-match approaches. Our extensive evaluation of prominent LLMs, including ChatGPT, GPT-4, Llama 2, and Code Llama, reveals 10 pivotal insights. This evaluation generates 4 billion inference tokens, incurred a total expense of 11,418 US dollars and 873 NVIDIA A100 GPU hours. Our findings highlight both the transformative potential of LLMs in this field and the challenges yet to be overcome

1,4-Diazo­niabicyclo­[2.2.2]octane bis­(2,4,6-trinitro­phenolate)

In the title compound, C6H14N2 2+·2C6H2N3O7 −, the cation possesses crystallographically imposed twofold rotation symmetry. In the crystal structure, the cation and anions are linked into a trimeric aggregate by inter­molecular N—H⋯O hydrogen bonds. The trimeric units are further connected by π–π inter­actions [centroid–centroid distances = 3.507 (2)–3.660 (3) Å], forming layers parallel to the bc plane