Approximated penalized maximum likelihood for exploratory factor analysis: an orthogonal case

The problem of penalized maximum likelihood (PML) for an exploratory factor analysis (EFA) model is studied in this paper. An EFA model is typically estimated using maximum likelihood and then the estimated loading matrix is rotated to obtain a sparse representation. Penalized maximum likelihood simultaneously fits the EFA model and produces a sparse loading matrix. To overcome some of the computational drawbacks of PML, an approximation to PML is proposed in this paper. It is further applied to an empirical dataset for illustration. A simulation study shows that the approximation naturally produces a sparse loading matrix and more accurately estimates the factor loadings and the covariance matrix, in the sense of having a lower mean squared error than factor rotations, under various conditions

A multifunctional tripodal fluorescent probe for the recognition of Cr3+, Al3+, Zn2+ and F− with controllable ESIPT processes

Three 4-(benzo[d]thiazol-2-yl)-2,5-dihydroxybenzaldehyde fluorophores were introduced to construct a tripodal multifunctional ESPIT fluorescence probe L. The fluorescent analysis revealed that probe L exhibited excellent recognition capabilities towards Cr3+, Al3+, Zn2+ and F− ions with large Stokes shifts. Furthermore, under optimal conditions, the detection limit of probe L towards Cr3+, Al3+, Zn2+ and F− were low, of the order of 10−8 M, which indicated that probe L was sensitive to these four ions. Interestingly, the fluorescent and 1H NMR titration experiments revealed that the recognition mechanism of probe L towards the ions Cr3+, Al3+, Zn2+ and F− were different. The presence of Cr3+ and Al3+ recovered the ESIPT, but the presence of Zn2+ trigger a moderate deprotonation of the phenolic OH and induced an ESIPT red-shifted (60 nm) emission wavelength. Finally, the presence of F− completely deprotonated the free phenolic OH and a remarkable red-shifted (130 nm) ESIPT emission was observed. In other words, the ESIPT process of probe L is controllable. Furthermore, the utility of probe L as a biosensor in living cells (PC3 cells) towards Cr3+, Al3+ and Zn2+ ions has been demonstrated

Investigation into antiepileptic effect of ganoderic acid A and its mechanism in seizure rats induced by pentylenetetrazole

Ganoderic acid A (GAA) exhibited neuron protection in in vitro epilepsy study, but no study has been done in vivo. Rats were administered (i.p.) pentylenetetrazole daily for 28 days to induce seizure. Rats with grade II or above of epileptic score were divided into three groups and given placebo, sodium valproate, or GAA treatment, respectively, for 7 days. The electrical signals of brain were monitored with electroencephalography (EGG); epileptic behavior was assessed using the Racine scale; morphological changes and apoptosis rate of cortical neurons were assessed with H&E staining and TUNEL staining, respectively. Protein expression of calcium-sensing receptor, p-ERK, p-JNK, and p-p38 in hippocampal tissue and Bcl-2, cleaved caspase-3, and Bax in cortical tissues was observed by Western blot and immunohistochemistry assay, respectively. After GAA treatment, apparent seizure-like EEG with significant arrhythmic disorder and spike waves was reduced or disappeared, and wave amplitude of EEG was reduced significantly. GAA showed similar effect with sodium valproate treatments on epilepsy. There were an apparent improvement of the epileptic behavior and a significant increase in the epileptic latency and shortening of the epileptic duration in the treatment group compared to control. GAA treatment ameliorated the nuclear pyknosis of neurons which appeared seriously in the epilepsy group. GAA treatment significantly reduced the cortical neuron apoptosis of epilepsy and the expression of calcium-sensing receptor, p-P38, p-JNK, cleaved caspase-3, and Bax but increased the expression of both p-ERK and Bcl-2. In conclusion, GAA treatment showed strong antiepileptic effect by decreasing apoptosis in cortical neuron and the expression of calcium-sensing receptor and stimulating the MAPK pathwa

High-efficiency polarization multiplexing metalenses

The polarization multiplexing technique is a well-established method that improves the communication capacity of an optical system. In this paper, we designed orthogonal linear and circular polarization multiplexing metalens using a library of rectangle TiO2 nanostructures. The former can independently focus x- and y-linearly polarized incident lights to designed positions with a focusing efficiency of 53.81% and 51.56%, respectively, whereas the latter with two preset focal points can independently control left and right circularly polarized incident lights with a focusing efficiency of 42.45% and 42.46%, respectively. We also show that both metalenses can produce diffraction-limited focal spots for four polarization states with no obvious distortion, which opens up new applications in polarization imaging and polarization detection

Preclinical Evidence for the Use of Sunitinib Malate in the Treatment of Plexiform Neurofibromas

Plexiform neurofibromas (pNF) are pathognomonic nerve and soft tissue tumors of neurofibromatosis type I (NF1), which are highly resistant to conventional chemotherapy and associated with significant morbidity/mortality. Disruption of aberrant SCF/c-Kit signaling emanating from the pNF microenvironment induced the first ever objective therapeutic responses in a recent phase 2 trial. Sunitinib malate is a potent, highly selective RTK inhibitor with activity against c-Kit, PDGFR, and VEGFR, which have also been implicated in the pathogenesis of these lesions. Here, we evaluate the efficacy of sunitinib malate in a preclinical Krox20;Nf1flox/− pNF murine model. Experimental Design Proliferation, β-hexosaminidase release (degranulation), and Erk1/2 phosphorylation were assessed in sunitinib treated Nf1+/− mast cells and fibroblasts, respectively. Krox20;Nf1flox/− mice with established pNF were treated sunitinib or PBS-vehicle control for a duration of 12 weeks. pNF metabolic activity was monitored by serial [18F]DG-PET/CT imaging. Results Sunitinib suppressed multiple in vitro gain-in-functions of Nf1+/− mast cells and fibroblasts and attenuated Erk1/2 phosphorylation. Sunitinib treated Krox20;Nf1flox/− mice exhibited significant reductions in pNF size, tumor number, and FDG uptake compared to control mice. Histopathology revealed reduced tumor cellularity and infiltrating mast cells, markedly diminished collagen deposition, and increased cellular apoptosis in sunitinib treated pNF. Conclusions Collectively, these results demonstrate the efficacy of sunitinib in reducing tumor burden in Krox20;Nf1flox/− mice. These preclinical findings demonstrate the utility of inhibiting multiple RTKs in pNF and provide insights into the design of future clinical trials

Characterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China

BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) is recognized as an important human diarrheal pathogen. Swine plays an important role as a carrier of this pathogen. In this study we determined the prevalence and characteristics of STEC from healthy swine collected between May 2011 and August 2012 from 3 cities/provinces in China. RESULTS: A total of 1003 samples, including 326 fecal, 351 small intestinal contents and 326 colon contents samples, was analyzed. Two hundred and fifty five samples were stx-positive by PCR and 93 STEC isolates were recovered from 62 stx-positive samples. Twelve O serogroups and 19 O:H serotypes including 6 serotypes (O100:H20/[H20], O143:H38/[H38], O87:H10, O172:H30/[H30], O159:H16, O9:H30/[H30]) rarely found in swine and ruminants were identified. All 93 STEC isolates harbored stx(2) only, all of which were stx(2e) subtype including 1 isolate being a new variant of stx(2e). 53.76%, 15.05% and 2.15% STEC isolates carried astA, hlyA and ehxA respectively. Four STEC isolates harbored the high-pathogenicity island. Of the 15 adherence-associated genes tested, 13 (eae, efa1, iha, lpfA(O113), lpfA(O157/OI-154), lpfA(O157/OI-141), toxB, saa, F4, F5, F6, F17 or F41) were all absent while 2 (paa and F18) were present in 7 and 4 STEC isolates respectively. The majority of the isolates were resistant to tetracycline (79.57%), nalidixic acid (78.49%), trimethoprim-sulfamethoxazole (73.12%) and kanamycin (55.91%). The STEC isolates were divided into 63 pulsed-field gel electrophoresis patterns and 21 sequence types (STs). Isolates of the same STs generally showed the same or similar drug resistance patterns. A higher proportion of STEC isolates from Chongqing showed multidrug resistance with one ST (ST3628) resistant to 14 antimicrobials. CONCLUSIONS: Our results indicate that swine is a significant reservoir of STEC strains in China. Based on comparison by serotypes and sequence types with human strains and presence of virulence genes, the swine STEC may have a low potential to cause human disease