An unusual S-adenosylmethionine synthetase gene from dinoflagellate is methylated

Background: S-Adenosylmethionine synthetase (AdoMetS) catalyzes the formation of S-Adenosylmethionine (AdoMet), the major methyl group donor in cells. AdoMet-mediated methylation of DNA is known to have regulatory effects on DNA transcription and chromosome structure. Transcription of environmental-responsive genes was demonstrated to be mediated via DNA methylation in dinoflagellates. Results: A full-length cDNA encoding AdoMetS was cloned from the dinoflagellate Crypthecodinium cohnii. Phylogenetic analysis suggests that the CcAdoMetS gene, is associated with the clade of higher plant orthrologues, and not to the clade of the animal orthrologues. Surprisingly, three extra stretches of residues ( 8 to 19 amino acids) were found on CcAdoMetS, when compared to other members of this usually conserved protein family. Modeled on the bacterial AdeMetS, two of the extra loops are located close to the methionine binding site. Despite this, the CcAdoMetS was able to rescue the corresponding mutant of budding yeast. Southern analysis, coupled with methylation-sensitive and insensitive enzyme digestion of C. cohnii genomic DNA, demonstrated that the AdoMetS gene is itself methylated. The increase in digestibility of methylation-sensitive enzymes on AdoMet synthetase gene observed following the addition of DNA methylation inhibitors L-ethionine and 5-azacytidine suggests the presence of cytosine methylation sites within CcAdoMetS gene. During the cell cycle, both the transcript and protein levels of CcAdoMetS peaked at the G1 phase. L- ethionine was able to delay the cell cycle at the entry of S phase. A cell cycle delay at the exit of G2/M phase was induced by 5-azacytidine. Conclusion: The present study demonstrates a major role of AdoMet-mediated DNA methylation in the regulation of cell proliferation and that the CcAdoMetS gene is itself methylated

Rockport Comprehensive Plan

This document was developed and prepared by Texas Target Communities (TxTC) at Texas A&M University in partnership with the City of Rockport, Texas Sea Grant, Texas A&M University - Corpus Christi, Texas A&M University - School of Law and Texas Tech University.Founded in 1871, the City of Rockport aims to continue growing economically and sustainably. Rockport is a resilient community dedicated to sustainable growth and attracting businesses to the area. Rockport is a charming town that offers a close-knit community feel and is a popular tourist destination for marine recreation, fairs, and exhibitions throughout the year. The Comprehensive Plan 2020-2040 is designed to guide the city of Rockport for its future growth. The guiding principles for this planning process were Rockport's vision statement and its corresponding goals, which were crafted by the task force. The goals focus on factors of growth and development including public participation, development considerations, transportation, community facilities, economic development, parks, and housing and social vulnerability

Efficient Option Pricing under Levy Processes, with CVA and FVA

We generalize the Piterbarg (2010) model to include 1) bilateral default risk as in Burgard and Kjaer (2012), and 2) jumps in the dynamics of the underlying asset using general classes of L'evy processes of exponential type. We develop an efficient explicit-implicit scheme for European options and barrier options taking CVA-FVA into account. We highlight the importance of this work in the context of trading, pricing and management a derivative portfolio given the trajectory of regulations

O papel do supervisor na criação e na dinamização de uma cultura reflexiva e colaborativa numa Instituição Particular de Solidariedade Social

Dissertação de Mestrado em Ciências da Educação - Especialização em Supervisão Pedagógica, apresentada à Escola Superior de Educação de Paula FrassinettiO projeto que denominamos de O Papel do Supervisor na Criação e na Dinamização de uma Cultura Reflexiva e Colaborativa numa Instituição Particular de Solidariedade Social insere-se numa Instituição Particular de Solidariedade Social com duas respostas sociais – Creche e Jardim de Infância – e que juntamente com mais quatro equipamentos similares fazem parte de um grupo que abrange outras áreas, nomeadamente o desporto e a habitação. É nossa pretensão analisar de que forma as práticas reflexivas e o trabalho colaborativo são trabalhadas pelas educadoras que colaboram neste equipamento, bem como de que forma o trabalho do supervisor pode ajudar à implementação de mecanismos de ajuda, acompanhamento e controle na sistematização de práticas pedagógicas reflexivas e de trabalho colaborativo e em equipa.The project denominated by O Papel do Supervisor na Criação e na Dinamização de uma Cultura Reflexiva e Colaborativa numa Instituição Particular de Solidariedade Social is part of a Private Institution of Social Solidarity with two social responses - Nursery & Kindergarten - and that along with four similar equipment are part of a group that includes other areas, like sport and housing. It is our intention to examine how reflective practice and collaborative work are worked by the educators who work on this equipment, as well as how the work of the supervisor can help the implementation of support mechanisms, monitoring and control in the systematization of reflective teaching practices and collaborative work and team