15 research outputs found
Genotipificación molecular de las cepas del virus sarampión en el Perú, 2018
Mr. Editor, in the year 2017, four countries of the Americas region reported confirmed cases of measles, namely: Argentina (3 cases), Canada (45 cases), the United States of America (120 cases) and the Republic Bolivariana de Venezuela (727 cases). In the first months of 2018, the following 9 countries have reported confirmed cases: Antigua and Barbuda (1 case), Brazil (14 cases), Canada (4 cases), Colombia (1), United States of America (13 cases), Guatemala (1 case), Mexico (4 cases), Peru (2 cases) and Venezuela (159 cases) (1).Sr. Editor, en el año 2017, cuatro países de la región de las Américas notificaron casos confirmados de sarampión, a saber: Argentina (3 casos), Canadá (45 casos), los Estados Unidos de América (120 casos) y la República Bolivariana de Venezuela (727 casos). En los primeros meses de 2018, los siguientes 9 países han notificado casos confirmados: Antigua y Barbuda (1 caso), Brasil (14 casos), Canadá (4 casos), Colombia (1), Estados Unidos de América (13 casos), Guatemala (1 caso), México (4 casos), Perú (2 casos) y Venezuela (159 casos)(1)
Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions
Single nucleotide variations shown across all three sequenced regions (<i>ialB</i>, <i>gltA</i> and <i>rpoB</i>).
Single nucleotide variations shown across all three sequenced regions (ialB, gltA and rpoB).</p
Fig 1 -
Sequence logo of the multiple sequence alignment of amino acid sequence for the identification of non-synonymous mutations: A) IalB; B) GltA; C) RpoB. Amino acids are colored according to their chemical properties: polar amino acids are green, basic amino acids are blue, acidic amino acids are red, and hydrophobic amino acids are black.</p
Predicted amino acid substitutions in <i>B</i>. <i>bacilliformis</i> presenting DNA differences compared to the <i>B</i>. <i>bacilliformis</i> reference strain KC583 (ATCC35685).
Predicted amino acid substitutions in B. bacilliformis presenting DNA differences compared to the B. bacilliformis reference strain KC583 (ATCC35685).</p
S1 Fig -
Single-nucleotide variations displayed along the aligned sequences of: A)ialB; B) gltA, consensus sequence includes only 782–1160 nucleotide sequences; C) rpoB, consensus sequence includes only 1451–2275 nucleotide sequences. (PDF)</p
Phylogenetic tree based on the <i>rpoB gene</i> from 10 <i>B</i>. <i>bacilliformis</i> isolates from Cajamarca and 10 from Ancash, and 17 genomes downloaded from the GenBank.
For the construction of the tree, the NJ method with 1,000 replicates was used. Bootstrap values are shown between branches and evolutionary distances are calculated using Kimura’s 2-parameter method. The tree was made in the MEGA 7.0 program. Red and green symbols highlight strains sequenced in the study. (PDF)</p
Concatenated phylogenetic tree based on the <i>ialB</i>, <i>gltA</i>, <i>rpoB genes</i> from 20 strains isolated from Cajamarca and Ancash, and 20 genomes downloaded from GenBank.
For the construction of the tree, the NJ method with 1,000 replicates was used. The Bootstrap values are displayed between the branches respectively. The tree was made in the MEGA 7.0 program.</p
Phylogenetic tree based on the <i>ialB</i> gene in 10 isolates from Cajamarca and 10 from Ancash and 17 genomes downloaded from the Genbank.
The construction of the tree used the NJ method with 1,000 replicates. The Bootstrap values are displayed between the branches respectively. The tree was made in the MEGA 7.0 program. Red and green symbols highlight strains sequenced in the study. (PDF)</p
Phylogenetic tree based on the <i>gltA</i> gene of 10 <i>B</i>. <i>bacilliformis</i> isolated in Cajamarca and 10 from Ancash; 17 genomes and 2 partial genes downloaded from the GenBank.
The construction of the tree used the NJ method with 1,000 replicates. Bootstrap values are shown between branches respectively. The tree was made in the MEGA 7.0 program. Red and green symbols highlight strains sequenced in the study. (PDF)</p