ANTICANCER ACTIVITY OF MICRO-ALGAE EXTRACT ON HUMAN CANCER CELL LINE (MG-63)

Objective: The objective of the present study is to assess the anticancer effect of limnic algae extract of KACC 2 (Karunya Algae Culture Collection) and KACC 23 against MG-63 cell line.Methods: Bioactive compounds were extracted from the algae culture by Soxhlet extraction method using methanol as solvent, and the extract was checked for its anticancer activity. Polyphenols were estimated using Folin-Ciocalteu reagent method. The constituents of the extract were analyzed using gas chromatography-mass spectrometry (GC-MS).Results: The results showed that KACC 2 had more phenolic content than KACC 23. The half maximal inhibitory concentration value for KACC 2 and 23 was found as 0.386 μl/ml and 0.285 μl/ml of extracts necessary for the 50% of cell death. GC-MS analysis revealed that the compounds with possible therapeutic effects are quercetin, stigmast-4-en-3-one, epoxygedunin, which has got anticancer activity.Conclusion: These observations point out that the algae extract of KACC 2 and KACC 23 has anticancer property and the phytoconstituents contributed to the anticancer effects

P-glycoprotein expression in oral lichen planus

<div><p>Abstract: Oral lichen planus (OLP) is a stress induced inflammatory condition with malignant potency. The mdr1 (multidrug resistance) is a stress gene overexpressed in cancerous conditions and its translated form, the p-glycoprotein efflux transporter is usually overexpressed with chemotherapy, leading to chemoresistance. OLP, a lesion with carcinogenic potency, is broadly classified into the asymptomatic reticular form and the aggressive erosive form. The objective of the study was to verify the expression level of p-glycoprotein in antifungal-treated and untreated reticular OLP, in untreated erosive OLP and erosive OLP patients treated with corticosteroid. Semi-quantitative reverse transcriptase polymerase chain reaction (SQ-RTPCR) and ELISA were performed on biopsy tissue samples to evaluate the mdr1 mRNA and protein expression of p-glycoprotein, respectively. The present study shows for the first time that mdr1 mRNA as well as its translated form p-glycoprotein are overexpressed in OLP subjects compared to healthy individuals. This overexpression is significantly higher in erosive than in reticular OLP patients, further confirming that the erosive form has higher risk for multidrug resistance. A higher expression is also observed in corticosteroid-treated erosive cases than similar untreated ones. The gradation of expression is in conformity with severity of the disease.</p></div