Effects of Full-Length Borealin on the Composition and Protein-Protein Interaction Activity of a Binary Chromosomal Passenger Complex

The chromosomal passenger complex (CPC) comprises at least four protein components and functions at various cellular localizations during different mitotic stages to ensure correct chromosome segregation and completion of cytokinesis. Borealin, the most recently identified member of the CPC, is an intrinsically unstructured protein of low solubility and stability. Recent reports have demonstrated the formation binary or ternary CPC sub-complexes incorporating short Borealin fragments in vitro. Using isothermal titration calorimetry, we show that full-length Borealin, instead of a Borealin fragment possessing the complete Survivin and INCENP-recognition sequence, is required for the composition of a Borealin-Survivin complex competent to interact with INCENP. In addition, we show evidence that full-length Borealin, which forms high-order oligomers in its isolated form, is a monomer in the Borealin-Survivin CPC sub-complex

Should Structured Exercise Be Promoted As a Model of Care? Dissemination of the Department of Veterans Affairs Gerofit Program

Exercise provides a wide range of health‐promoting benefits, but support is limited for clinical programs that use exercise as a means of health promotion. This stands in contrast to restorative or rehabilitative exercise, which is considered an essential medical service. We propose that there is a place for ongoing, structured wellness and health promotion programs, with exercise as the primary therapeutic focus. Such programs have long‐lasting health benefits, are easily implementable, and are associated with high levels of participant satisfaction. We describe the dissemination and implementation of a long‐standing exercise and health promotion program, Gerofit, for which significant gains in physical function that have been maintained over 5 years of follow‐up, improvements in well‐being, and a 10‐year 25% survival benefit among program adherents have been documented. The program has been replicated at 6 Veterans Affairs Medical Centers. The pooled characteristics of enrolled participants (n = 691) demonstrate substantial baseline functional impairment (usual gait speed 1.05 ± 0.3 m/s, 8‐foot up and go 8.7 ± 6.7 seconds, 30‐second chair stands 10.7 ± 5.1, 6‐minute walk distance 404.31 ± 141.9 m), highlighting the need for such programs. Change scores over baseline for 3, 6, and 12 months of follow‐up are clinically and statistically significant (P < .05 all measures) and replicate findings from the parent program. Patient satisfaction ratings of high ranged from 88% to 94%. We describe the implementation process and present 1‐year outcomes. We suggest that such programs be considered essential elements of healthcare systems

Mobility Improvements Are Found in Older Veterans After 6 Months of Gerofit Regardless of Body Mass Index Classification

Veterans represent a unique population of older adults, as they are more likely to self-report a disability and be overweight or obese compared with the general population. We sought to compare changes in mobility function across the obesity spectrum in older veterans participating in 6 months of Gerofit, a clinical exercise program. A total of 270 veterans (mean age: 74 years) completed baseline, 3-, and 6-month mobility assessments and were divided post hoc into groups: normal weight, overweight, and obese. The mobility assessments included 10-m walk time, 6-min walk distance, 30-s chair stands, and 8-foot up-and-go time. No significant weight × time interactions were found for any measure. However, clinically significant improvements of 7–20% were found for all mobility measures from baseline to 3 months and maintained at 6 months (all ps <05). Six months of participation in Gerofit, if enacted nationwide, appears to be one way to improve mobility in older veterans at high risk fordisability, regardless of weight status

Economic Load Dispatch Using a Chemotactic Differential Evolution Algorithm

Abstract. This paper presents a novel stochastic optimization approach to solve constrained economic load dispatch (ELD) problem using Hybrid Bacterial Foraging-Differential Evolution optimization algorithm. In this hybrid approach computational chemotaxis of BFOA, which may also be viewed as a stochastic gradient search, has been coupled with DE type mutation and crossover of the optimization agents. The proposed methodology easily takes care of solving non-convex economic load dispatch problems along with different constraints like transmission losses, dynamic operation constraints (ramp rate limits) and prohibited operating zones. Simulations were performed over various standard test systems with different number of generating units and comparisons are performed with other existing relevant approaches. The findings affirmed the robustness and proficiency of the proposed methodology over other existing techniques. 1