TP53RK and BCL2L2 are direct targets of miR-630.

<p>(A) Predicted binding sites of miR-630 in the 3′-UTRs of TP53RK and BCL2L2 (B and C) Fluorescence was significantly reduced in the miR-630 mimics and wild-type dual-luciferase reporter plasmid-transfected group, while it showed no significant change in the miR-630 mimics and mutant dual-luciferase reporter plasmid-transfected group (D) TP53RK and BCL2L2 protein expression levels decreased in miR-630-transfected cells. Error bars represent the mean of three separate determinations ± standard deviation (SD). Asterisk indicates statistically significant changes: * (P < 0.05), ** (P < 0.01).</p

Inhibition of miR-630 led to decreased IR sensitivity.

<p>(A) Inhibition of miR-630 significantly decreased IR-induced inhibition rate (B and C) Inhibition of miR-630 strongly decreased caspase 3 and caspase 6 activities following IR exposure. Error bars represent the mean of three separate determinations ± standard deviation (SD). Asterisk indicates statistically significant changes: * (P < 0.05), ** (P < 0.01).</p

miR-630 overexpression enhanced ionic radiation (IR)-induced cytotoxicity.

<p>(A) miR-630 significantly increased IR-induced inhibition rate (B and C) miR-630 strongly increased caspase 3 and caspase 6 activities following IR exposure (D and E) miR-630 significantly increased apoptosis rate after IR exposure. Error bars represent the mean of three separate determinations ± standard deviation (SD). Asterisk indicates statistically significant changes: * (P < 0.05), ** (P < 0.01).</p

TP53RK and BCL2L2 suppress the effects of miR-630 overexpression.

<p>(A) TP53RK or BCL2L2 protein caused a significantly decreased IR-induced inhibition rate in miR-630 transfected cell lines. (B and C) TP53RK or BCL2L2 protein decreased the fold of caspase 3 and caspase 6 activities following IR exposure in miR-630 transfected cell lines. Error bars represent the mean of three separate determinations ± standard deviation (SD). Asterisk indicates statistically significant changes: * (P < 0.05), ** (P < 0.01).</p

DNA methylation status and transcription factor CREB mediated miR-630 biogenesis.

<p>(A) 5-aza-CdR significantly upregulated miR-630 expression (B) CREB was predicted to bind to the 5′-UTR of miR-630 host gene ARIH1 (C) CREB within the ARIH1 promoter region induced luciferase activity and mutation of predicted CREB binding sites reversed its effect (D) ChIP confirmed the binding of CREB to the ARIH1 promoter. Error bars represent the mean of three separate determinations ± standard deviation (SD). Asterisk indicates statistically significant changes: * (P < 0.05), ** (P < 0.01).</p

miR-630 expression levels reduced after radiation.

<p>(A and B) Endogenous miR-630 expression and IR-induced inhibition rate of serial CRC cell lines (C) miR-630 level decrease after radiation compared with their initial level. Error bars represent the mean of three separate determinations ± standard deviation (SD). Asterisk indicates statistically significant changes: * (P < 0.05), ** (P < 0.01).</p