42 research outputs found
Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides
Developing an efficient
strategy to enrich the low abundance phosphopeptides
before mass spectrometry detection is a vital preprocessing step in
phosphoproteomics. In this work, we synthesized an adenosine phosphate-Ti<sup>4+</sup> functionalized magnetic mesoporous graphene oxide nanocomposite
(denoted as MG@mSiO<sub>2</sub>-ATP-Ti<sup>4+</sup>) to selectively
extract phosphorylated peptides from complex biological samples based
on the immobilized metal ion affinity chromatography (IMAC). Mesoporous
silica was coated on the substrate material of magnetic graphene oxide
and then the ATP containing three phosphate groups was grafted on
the inwall of mesoporous channels as chelating ligands to immobilize
the Ti<sup>4+</sup> cations. With favorable properties, such as large
surface area and good hydrophilicity and size-exclusion effect, the
MG@mSiO<sub>2</sub>-ATP-Ti<sup>4+</sup> exhibited excellent sensitivity
and selectivity toward phosphopeptides whether in low concentration
of β-casein digest (20 amol μL<sup>–1</sup>, 4
fmol) or the digest mixture of β-casein and bovine serum albumin
(with molar ratio of 1:1000) as well as good reusability. Furthermore,
MG@mSiO<sub>2</sub>-ATP-Ti<sup>4+</sup> could also be applied in the
selective enrichment of phosphorylated peptides from nonfat milk digest
and human saliva and serum
In Vivo Delivery of RNAi by Reducible Interfering Nanoparticles (iNOPs)
RNA interference (RNAi) has considerable
potential as a therapeutic
strategy, but the development of efficient in vivo RNA delivery methods
remains challenging. To this end, we designed and synthesized chemically
modified interfering nanoparticles (iNOPs) composed of functionalized
poly-l-lysine dendrimers modified with reducible spacers
to facilitate release of small interfering RNAs (siRNAs) in vivo.
We show that the novel siRNA–iNOP complexes mediate efficient
gene-specific RNAi in cultured cells and in mice, where they display
enhanced tissue-targeting capabilities. At a clinically feasible dose
of 1 mg kg<sup>–1</sup>, apolipoprotein B (apoB) siRNA–iNOP
complexes achieved ∼40–45% reduction of liver apoB mRNA
and plasma apoB protein levels within 48 h of administration to mice,
without apparent toxicity. Collectively, these findings demonstrate
that siRNA delivery by the modified reducible iNOPs can provide a
clinically significant and potentially tissue-specific new approach
for RNAi therapy
Selection of Amino Acids and the Biomimetic Synthesis of Amido Bond in the Presence of β-CD
<div><p></p><p>A new method was developed to construct a special amido bond in the presence of β-cyclodextrin. This process is similar to peptide synthesis in organisms. NMR experiments were performed to investigate the possible mechanism. This work has potential application in biomimetic peptide synthesis.</p>
<p>[Supplementary materials are available for this article. Go to the publisher's online edition of <i>Synthetic Communications</i>® for the following free supplemental resource(s): Full experimental and spectral details.]</p>
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Carbonate-Based Zeolitic Imidazolate Framework for Highly Selective CO<sub>2</sub> Capture
In
this study, we report the formation of a new crystal structure, ZIF-CO<sub>3</sub>-1, which results from the reaction of Zn<sup>2+</sup>, 2-methylimidazole,
and carbonate. ZIF-CO<sub>3</sub>-1 can be synthesized solvothermally
in <i>N</i>,<i>N</i>-dimethylformamide (DMF)/water
(H<sub>2</sub>O) or by utilizing of CO<sub>2</sub> gas at various
temperatures in DMF/H<sub>2</sub>O or H<sub>2</sub>O. This reaction
selectively consumes CO<sub>2</sub> because CO<sub>2</sub> is incorporated
in the ZIF as carbonate. CO<sub>2</sub> can be quantitatively released
by acidifying the ZIF. Powder X-ray diffraction, single-crystal X-ray
diffraction, FTIR spectroscopy, scanning electron microscopy, elemental
analysis, and thermogravimetric analysis were used to characterize
the ZIF structure. ZIF-CO<sub>3</sub>-1 (chemical formula C<sub>9</sub>H<sub>10</sub>N<sub>4</sub>O<sub>3</sub>Zn<sub>2</sub>), crystallizes
in the orthorhombic crystal system with noncentrosymmetric space group <i>Pba</i>2
Effect of polysaccharides extracted from <i>Sipunculus nudus</i> (SNP) on the lifespan and immune damage repair of <i>Drosophila melanogaster</i> exposed to Cd (VI)
<p>The water-soluble polysaccharides extracted from <i>Sipunculus nudus</i> (SNP) was investigated on the lifespan and immune damage repair of <i>Drosophila melanogaster</i> exposed to Cd (VI). SNP increased superoxyde dismutase (SOD), nitrogen monoxide (NO), glutathione peroxidase (GSH-Px) and total anti-oxidation competence (T-AOC), with decreased malondialdehyde (MDA) on <i>D. melanogaster</i> demonstrated that SNP could <i>attenuate</i> <i>oxidative damage of D. melanogaster Exposed to Cd (VI).</i> Real-time PCR and western blot analysis showed that SNP enhanced the gene expression of Diptericin, Drosomycin, Defensin, PGRP-LC and the protein level of Toll, p-JNK and Relish, that suggested the promoting effect of SNP on the immune damage repair of <i>D. melanogaster</i> exposed to Cd (VI). The increased level of Indy, Parkin and AMPK indicated the regulated effect of SNP on the longevity-related pathways through ageing-related moleculars of <i>D. melanogaster</i> exposed to Cd (VI). These results suggested that SNP could also improve the lifespan of <i>D. melanogaster</i> exposed to Cd (VI).</p
Carbonate-Based Zeolitic Imidazolate Framework for Highly Selective CO<sub>2</sub> Capture
In
this study, we report the formation of a new crystal structure, ZIF-CO<sub>3</sub>-1, which results from the reaction of Zn<sup>2+</sup>, 2-methylimidazole,
and carbonate. ZIF-CO<sub>3</sub>-1 can be synthesized solvothermally
in <i>N</i>,<i>N</i>-dimethylformamide (DMF)/water
(H<sub>2</sub>O) or by utilizing of CO<sub>2</sub> gas at various
temperatures in DMF/H<sub>2</sub>O or H<sub>2</sub>O. This reaction
selectively consumes CO<sub>2</sub> because CO<sub>2</sub> is incorporated
in the ZIF as carbonate. CO<sub>2</sub> can be quantitatively released
by acidifying the ZIF. Powder X-ray diffraction, single-crystal X-ray
diffraction, FTIR spectroscopy, scanning electron microscopy, elemental
analysis, and thermogravimetric analysis were used to characterize
the ZIF structure. ZIF-CO<sub>3</sub>-1 (chemical formula C<sub>9</sub>H<sub>10</sub>N<sub>4</sub>O<sub>3</sub>Zn<sub>2</sub>), crystallizes
in the orthorhombic crystal system with noncentrosymmetric space group <i>Pba</i>2
A Family of Flexible Lanthanide Bipyridinedicarboxylate Metal–Organic Frameworks Showing Reversible Single-Crystal to Single-Crystal Transformations
A family of flexible lanthanide metal–organic
frameworks,
[Ln<sub>2</sub>(bpydc)<sub>3</sub>(H<sub>2</sub>O)<sub>3</sub>]·nDMF
(denoted as SUMOF-6-Ln; Ln = Nd, Sm, Eu, Gd, Tb, Dy, Ho, and Er, H<sub>2</sub>bpydc =2,2′-bipyridine-5,5′-dicarboxylic acid),
was synthesized and characterized. SUMOF-6-Ln has a monoclinic space
group <i>P</i>2<sub>1</sub><i>/c</i>. The three-dimensional
framework contains chains of LnO<sub><i>n</i></sub> (<i>n</i> = 7–8) polyhedra connected through the bpydc linkers
forming 1D rhombic channels along the <i>c</i>-axis. SUMOF-6-Ln
showed reversible breathing phenomenon upon desorption/adsorption
of the solvent, with up to 27% changes of the unit cell dimensions
and 23% changes of the unit cell volume. Single crystal X-ray diffraction
(XRD) revealed that the desolvation and resolvation of SUMOF-6-Ln
occurred via single-crystal to single-crystal transformations. The
thermal behavior of SUMOF-6-Sm was also examined. SUMOF-6-Eu and SUMOF-6-Tb
showed solid-state luminescent properties
A Family of Flexible Lanthanide Bipyridinedicarboxylate Metal–Organic Frameworks Showing Reversible Single-Crystal to Single-Crystal Transformations
A family of flexible lanthanide metal–organic
frameworks,
[Ln<sub>2</sub>(bpydc)<sub>3</sub>(H<sub>2</sub>O)<sub>3</sub>]·nDMF
(denoted as SUMOF-6-Ln; Ln = Nd, Sm, Eu, Gd, Tb, Dy, Ho, and Er, H<sub>2</sub>bpydc =2,2′-bipyridine-5,5′-dicarboxylic acid),
was synthesized and characterized. SUMOF-6-Ln has a monoclinic space
group <i>P</i>2<sub>1</sub><i>/c</i>. The three-dimensional
framework contains chains of LnO<sub><i>n</i></sub> (<i>n</i> = 7–8) polyhedra connected through the bpydc linkers
forming 1D rhombic channels along the <i>c</i>-axis. SUMOF-6-Ln
showed reversible breathing phenomenon upon desorption/adsorption
of the solvent, with up to 27% changes of the unit cell dimensions
and 23% changes of the unit cell volume. Single crystal X-ray diffraction
(XRD) revealed that the desolvation and resolvation of SUMOF-6-Ln
occurred via single-crystal to single-crystal transformations. The
thermal behavior of SUMOF-6-Sm was also examined. SUMOF-6-Eu and SUMOF-6-Tb
showed solid-state luminescent properties
Additional file 1 of The effect of haloperidol’s perioperative application on postoperative delirium in elderly patients: a systematic review and meta-analysis
Supplementary Material 1: PRISMA checklis
Piezofluorochromic Metal–Organic Framework: A Microscissor Lift
We
have successfully constructed a metal–organic framework,
denoted as PCN-128W, starting from chromophoric linker and zirconium
salt. PCN-128W exhibits interesting piezofluorochromic behavior, the
color reversibly changes from white to yellow and so does the emission
maximum from 470 to 538 nm. The stepwise fluorescence change was monitored
by fluorospectroscopy which demonstrated gradual shift of the emission
maximum when sequential compression was exerted. In order to gain
deep insights into the piezofluorochromic mechanism, both the white
and yellow phases are structurally characterized