3 research outputs found
Organocatalytic Enantioselective Formal [4 + 2] Cycloaddition of Enones with Cyclic <i>N</i>‑Sulfonylimines and Methylene Chromene for Chiral Spirocyclic Compounds
A highly enantioselective
synthesis of spirocycles and bridged
rings has been developed through a formal [4 + 2] cycloaddition reaction
between enones and <i>N</i>-sulfonylimines. The unprecedented
strategy has been realized utilizing <i>N</i>-sulfonylimine
as a novel dienophile through enamine–iminium tautomerism of <i>N</i>-sulfonylimine. In addition, a γ,ε-regioselective
cycloaddition reaction proceeded by employing methylene chromene species
as dienophiles
Influence of hydrothermal treatment on the microstructure and oxidation resistance of a Zn<sub>4</sub>B<sub>2</sub>O<sub>7</sub>·H<sub>2</sub>O (4ZnO·B<sub>2</sub>O<sub>3</sub>·H<sub>2</sub>O) coating for C/C composites
<p>Antioxidant modification for C/C composites by <i>in situ</i> hydrothermal synthesise at 140 °C of a 4ZnO·B<sub>2</sub>O<sub>3</sub>·H<sub>2</sub>O crystallite coating has been successfully achieved. The influence of hydrothermal time on the phase composition, microstructure of the as-prepared Zn<sub>4</sub>B<sub>2</sub>O<sub>7</sub>·H<sub>2</sub>O (4ZnO·B<sub>2</sub>O<sub>3</sub>·H<sub>2</sub>O), and its antioxidant modification for C/C composites were investigated. Samples were characterised by XRD, SEM, isothermal oxidation test and TG-DSC. Results show that, 4ZnO·B<sub>2</sub>O<sub>3</sub>·H<sub>2</sub>O crystalline coating is achieved on the surface of C/C composites after the hydrothermal treatment at 140 °C for time in the range of 2–12 h. A smooth and crack-free 4ZnO·B<sub>2</sub>O<sub>3</sub>·H<sub>2</sub>O layer can be obtained when the hydrothermal time reaches 8 h. Isothermal oxidation test demonstrates that the oxidation resistance of C/C composites is improved. The as-modified composites exhibit only 1.52 g·cm<sup>−2</sup> weight loss after oxidation at 600 °C for 15 h, while the non-modified one shows a 6.57 g·cm<sup>−2</sup> weight loss after only 10 h oxidation. For the uncoated C/C composite the oxidation rate is approximately linear with time (non-protective oxidation), thus at 15 h exposure one can estimate the mass loss to be 6.57 g·cm<sup>−2</sup> after 10 h for direct comparison with the coated samples.</p
Minimum Distance Between Two Epitopes in Sandwich Immunoassays for Small Molecules
The pursuit of the limit between dimensionalities is
a scientific
goal with high applicability. Sandwich immunoassay, usually based
on two antibodies binding two epitopes, is one of the most popular
mainstay tools in both academic and industrial fields. Herein, we
determined and evaluated the minimum distance of two epitopes in sandwich
immunoassays for small molecules. Briefly, nine model analytes comprising
two hapten epitopes, that is, melamine (MEL) and p-nitroaniline (NIA), were designed by increasing the linear chain
linkers brick by brick. Two groups of monoclonal antibodies (mAbs)
were produced with different recognition properties toward MEL and
NIA using 12 new haptens with different spacer arms. The results indicated
that two epitopes of the analyte with a distance of only 2.4 Å
could be simultaneously bound by two mAbs, which is the known limit
of epitope distance in sandwich immunoassays thus far. We further
found that an epitope distance of below 8.8 Å for the analyte
generally induces noticeable steric hindrance of antibodies, preventing
a sandwich immunoassay with high probability. These observations were
investigated and evaluated by molecular docking, molecular dynamics,
and surface plasmon resonance and using model and real analytes. Altogether,
we determined the minimum distance of two epitopes and explored the
molecular mechanism of the antibody–analyte–antibody
ternary complex in sandwich immunoassays, providing a theoretical
basis for hapten design, antibody discovery and development, and sandwich
immunoassay establishment for small molecules