Protein-Protein Affinity Determination by Quantitative FRET Quenching.

The molecular dissociation constant, Kd, is a well-established parameter to quantitate the affinity of protein-protein or other molecular interactions. Recently, we reported the theoretical basis and experimental procedure for Kd determination using a quantitative FRET method. Here we report a new development of Kd determination by measuring the reduction in donor fluorescence due to acceptor quenching in FRET. A new method of Kd determination was developed from the quantitative measurement of donor fluorescence quenching. The estimated Kd values of SUMO1-Ubc9 interaction based on this method are in good agreement with those determined by other technologies, including FRET acceptor emission. Thus, the acceptor-quenched approach can be used as a complement to the previously developed acceptor excitation method. The new methodology has more general applications regardless whether the acceptor is an excitable fluorophore or a quencher. Thus, these developments provide a complete methodology for protein or other molecule interaction affinity determinations in solution

Self-Supported Sheets-on-Wire CuO@Ni(OH)2/Zn(OH)2 Nanoarrays for High-Performance Flexible Quasi-Solid-State Supercapacitor

Transition metal hydroxides have attracted a lot of attention as the electrode materials for supercapacitors owing to their relatively high theoretical capacity, low cost, and facile preparation methods. However, their low intrinsic conductivity deteriorates their high-rate performance and cycling stability. Here, self-supported sheets-on-wire CuO@Ni(OH)2/Zn(OH)2 (CuO@NiZn) composite nanowire arrays were successfully grown on copper foam. The CuO nanowire backbone provided enhanced structural stability and a highly efficient electron-conducting pathway from the active hydroxide nanosheets to the current collector. The resulting CuO@NiZn as the battery-type electrode for supercapacitor application delivered a high capacity of 306.2 mAh g−1 at a current density of 0.8 A g−1 and a very stable capacity of 195.1 mAh g−1 at 4 A g−1 for 10,000 charge–discharge cycles. Furthermore, a quasi-solid-state hybrid supercapacitor (qss HSC) was assembled with active carbon, exhibiting 125.3 mAh g−1 at 0.8 A g−1 and a capacity of 41.6 mAh g−1 at 4 A g−1 for 5000 charge–discharge cycles. Furthermore, the qss HSC was able to deliver a high energy density of about 116.0 Wh kg−1. Even at the highest power density of 7.8 kW kg−1, an energy density of 20.5 Wh kg−1 could still be obtained. Finally, 14 red light-emitting diodes were lit up by a single qss HSC at different bending states, showing good potential for flexible energy storage applications

Temporal-spatial variation and regulatory mechanism of carbon budgets in territorial space through the lens of carbon balance: A case of the middle reaches of the Yangtze River urban agglomerations, China

As China’s largest cross-regional urban agglomerations, the middle reaches of the Yangtze River urban agglomerations (MRYRUA) possess both significant societal carbon source volume and ecological carbon sequestration capacity. Nevertheless, with the uncontrolled expansion of urban energy consumption activities and the industry migration from eastern coastal regions to inland cities, the carbon budget pattern of territorial space is increasingly unbalanced in the MRYRUA. To achieve low-carbon regulation, this study utilized land use and energy consumption data from 31 cities within the MRYRUA to establish a “carbon source-carbon sink” quantification and spatiotemporal exploration model, revealing the spatial-temporal variation of carbon budgets from 2005 to 2020. Furthermore, we developed a carbon balance indicator analysis system by employing the carbon offset rate (COR), carbon productivity (CP), Gini coefficient, ecological support coefficient (ESC), economic contribution coefficient (ECC), and functional zoning was performed. Finally, using the GM (1,1) model, we derived the carbon budget pattern for 2050 and explored the differentiated regulatory mechanisms under the carbon balance perspective. The results indicated that: (1) The MRYRUA’s territorial carbon budgets have increased annually, displaying a spatial distribution pattern with the highest values in the central region, followed by the northwest, and the lowest in the southeast near water bodies. The spatiotemporal differentiation effects manifest as an east–west axial development trend, with spatiotemporal clustering effects demonstrating a propensity for outward dispersion from the northern hot spot radiation core. (2) The MRYRUA’s COR has consistently remained below 10% and decreased annually, while the CP has shown a yearly increase at an accelerating rate. The ESC and ECC exhibit evident spatial heterogeneity among cities. In response to the carbon emission economic benefits and carbon sequestration ecological carrying capacity reflected by carbon balance indicators, each city was classified into low-carbon economic zones, carbon intensity control zones, carbon sink functional zones, and high-carbon optimization zones. (3) From 2020 to 2050, the polarization trend of the carbon budget pattern continues to intensify. Subsequently, we have established a differentiated territorial spatial carbon balance regulatory mechanism. This mechanism strengthens the leading role of low-carbon economic zones in the green low-carbon transition, moderately retains the carbon sink functional zones in the southeast with solid carbon fixation capabilities, and promotes the transition of the northern carbon intensity control zones and high-carbon optimization zones to low-carbon economic zones. The research findings provide a scientific basis for formulating territorial spatial planning policies from a carbon neutrality perspective

Coordinated power-water optimization for precision irrigation among distribution network and agricultural parks

With more and more agricultural parks are integrated to the electrical distribution network, the disordered characteristics of farmers’ irrigation water consumption will not only increase the cost of water and electricity, but also cause security problems in traditional term of rural distribution network, such as large short-term load fluctuation, overload and transformer load imbalance. In this context, this paper proposes a double-layer master–slave game model of power-water interaction and coordination between distribution network and agricultural irrigation park under photovoltaic (PV) assisted agricultural precision irrigation. The upper layer (electrical distribution network) plays an interactive game with the lower layer (agricultural irrigation parks) by establishing effective time-of-use (TOU) price. On the one hand, the electrical distribution network takes the operation security as the optimization objective, it could optimize line loss by network configuration and guides agricultural irrigation parks to consume electricity orderly by formulating electricity prices. On the other hand, agricultural irrigation parks take satisfaction and cost as the optimization objective after receiving TOU price from upper layer, and regulates the operation time and intensity of irrigation pumps to realize precise irrigation. Finally, an example is given to verify the model on the modified 148-node system. It is determined that the model can effectively support the safe and reliable operation of rural distribution network while realizing the transformation of agricultural irrigation water use from low efficiency to economical and high efficiency, so as to achieve win–win between agricultural irrigation park and distribution network

Enhancing Resilience and Reliability of Active Distribution Networks through Accurate Fault Location and Novel Pilot Protection Method

The integration of distributed generation (DG) into the decentralized access of the distribution network transforms the existing structure into an active distribution network. The alteration in fault characteristics poses significant challenges to the coordinated operation of relay protection. Fault location within the distribution network plays a vital role in facilitating fault recovery and enhancing the resilience of the power system. It proves instrumental in improving the network’s ability to withstand extreme disasters, thereby enhancing the reliability of power distribution. Therefore, this paper provides a detailed analysis of the voltage fault components occurring during various fault types within an active distribution network. Building upon the identified characteristics of voltage fault components, a novel approach for the longitudinal protection of active distribution networks is proposed. This method involves comparing the calculated values of voltage fault components with their actual values. The proposed approach is applicable to various fault scenarios, including short-circuit faults, line break faults, and recurring faults. It exhibits advantages such as insensitivity to the penetration of distributed power supplies and robustness in withstanding transition resistance. The simulation results validate the effectiveness of the proposed method, affirming its applicability to diverse protection requirements within active distribution networks