Analysis of the Fungal Community in Ziziphi Spinosae Semen through High-Throughput Sequencing

Ziziphi Spinosae Semen (ZSS) has been widely used in traditional Chinese medicine system for decades. Under proper humidity and temperature, ZSS is easily contaminated by fungi and mycotoxins during harvest, storage, and transport, thereby posing a considerable threat to consumer health. In this study, we first used the Illumina MiSeq PE250 platform and targeted the internal transcribed spacer 2 sequences to investigate the presence of fungi in moldy and normal ZSS samples collected from five producing areas in China. Results showed that all 14 samples tested were contaminated by fungi. Ascomycota was the dominant fungus at the phylum level, accounting for 64.36⁻99.74% of the fungal reads. At the genus level, Aspergillus, Candida, and Wallemia were the most predominant genera, with the relative abundances of 13.52⁻87.87%, 0.42⁻64.56%, and 0.06⁻34.31%, respectively. Meanwhile, 70 fungal taxa were identified at the species level. Among these taxa, three potential mycotoxin-producing fungi, namely, Aspergillus flavus, A. fumigatus, and Penicillium citrinum that account for 0.30⁻36.29%, 0.04⁻7.37%, and 0.01⁻0.80% of the fungal reads, respectively, were detected in all ZSS samples. Moreover, significant differences in fungal communities were observed in the moldy and normal ZSS samples. In conclusion, our results indicated that amplicon sequencing is feasible for the detection and analysis of the fungal community in the ZSS samples. This study used a new approach to survey the fungal contamination in herbal materials. This new approach can provide early warning for mycotoxin contamination in herbal materials, thereby ensuring drug efficacy and safety

A Comprehensive Review of Stratification and Rollover Behavior of Liquefied Natural Gas in Storage Tanks

Liquefied natural gas (LNG), as cleaner transitional energy than coal, is becoming increasingly prominent in the energy structure of various countries based on their low-carbon background, and its demand has grown rapidly worldwide. Storage tanks are the most commonly used LNG storage facilities. Owing to a variety of internal composition and external environmental factors, the instability phenomenon of LNG in the tanks may occur during storage, leading to potential safety risks. An in-depth understanding of the stratification and rollover behavior of LNG is therefore required to ensure and promote efficient utilization and stable storage of LNG. This paper provides a comprehensive review of the current state of LNG stratification and rollover behavior. The factors for causing LNG stratification and rollover behavior in the storage tanks are summarized, methods for characterizing stratification and rollover behavior are discussed, and measures for inhibiting the instability phenomenon of LNG in the tanks are highlighted. Future researchers should conduct further work in enriching the factors affecting the stratification and rollover behavior, improving characterization methods, and developing inhibition measures to ensure the safe and stable storage of LNG

Different bone sites-specific response to diabetes rat models: Bone density, histology and microarchitecture.

BACKGROUND AND PURPOSE:Diabetes mellitus (DM) is the most common metabolic disorder that is characterized by hyperglycemia, it can be categorized by T1DM and T2DM. T1DM is also reported to cause bone loss. However, most reports regarding this aspect of T1DM have only investigated a single site; a comparison of bone loss from different areas of the body is still lacking. METHODS:Thirty-five 12-week-old Sprague Dawley® (SD) rats were separated to seven groups. Five rats were euthanized without any surgery at 0 weeks for histological examination and determination of baseline characteristics. In 15 of the rats, DM was induced via Streptozotocin (STZ)-injection, and they were separated to 3 groups (4 weeks, 8 weeks and 12 weeks after STZ-injection). The remaining 15 rats were used as the control group (4 weeks, 8 weeks and 12 weeks after saline-injection). We tested bone-mass loss at four skeletal sites, the tibia, the femur greater trochanter, the spine, and the mandibular bones using micro-computed tomography (CT) and histological tests. RESULTS:Tibia was influenced the most obvious(BV/TV decreased by 27.3%, 52.5%, and 81.2% at 4 weeks, 8 weeks, and 12 weeks, respectively. p<0.05). In contrast, the other three sites were influenced to a lesser extent and bone loss became prominent at a later time point according to the histological and micro-CT tests(Femur: BV/TV did not decrease significantly at the first month or second month. However, and decreased by 49.4% at the third month, P<0.05. Mandible: the BV/TV only decreased by 6.5% at 1 month after STZ-injection. There was still a significant difference between the second and third months. The BV/TV decreased by 47.0% and 68.1% at 2 months and 3 months, respectively, (p<0.05) Spine: the BV/TV only decreased by 6.7%. However, significant change was observed in the spine at the second month and third month after STZ injection. The BV/TV decreased by 45.4% and 64.3%, respectively, p<0.05). CONCLUSION:The results indicate that T1DM can severely influence the bone structure of the 4 skeletal sites. Further, areas with dense trabecular bones were influenced less and at a later time point in comparison to the tibial region. CLINICAL RELEVANCE:Our research can serve as a guide to help increase the success rate of implant treatment, and help decrease the fracture risk in different bone types with greater accuracy

Residue detection and correlation analysis of multiple neonicotinoid insecticides and their metabolites in edible herbs

In this work, a green analytical method was established for the simultaneous extraction and detection of 20 analytes–10 neonicotinoid insecticides and their 10 major toxic metabolites in edible herbs. QuEChERS and LC-MS/MS were used to analyze the 20 analytes in five edible herbs. The residues of the 20 neonicotinoid insecticides and their metabolites in 109 herbal samples were detected, of which 90 samples were positive, and the residue of total neonicotinoid insecticides ranged from 0.26 to 139.28 μg/kg. Acetamiprid (77.06 %, ≤85.95 μg/kg), imidacloprid (67.89 %, ≤32.49 μg/kg) and their metabolites (N-desmethyl-acetamiprid (44.04 %, ≤18.42 μg/kg) and desnitro imidacloprid (48.62 %, ≤16.55 μg/kg) were most frequently detected in herbs. Significant positive correlations were found between imidacloprid/acetamiprid and their metabolites in Lycii fructus and Citri reticulatae pericarpium. Therefore, more attention may be given to the neonicotinoid insecticide residues in edible herbs in the future

Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model

Introduction: Zearalenone-14-glucoside (Z14G) is a modified mycotoxin that widely contaminates food across the world. Our preliminary experiment showed that Z14G degrades to zearalenone (ZEN) in the intestine exerting toxicity. Notably, oral administration of Z14G in rats induces intestinal nodular lymphatic hyperplasia. Objectives: To investigate the mechanism of Z14G intestinal toxicity and how it differs from ZEN toxicity. We conducted a precise toxicology study on the intestine of rats exposed to Z14G and ZEN using multi-omics technology. Methods: Rats were exposed to ZEN (5 mg/kg), Z14G-L (5 mg/kg), Z14G-H (10 mg/kg), and pseudo germ free (PGF)-Z14G-H (10 mg/kg) for 14 days. Histopathological studies were performed on intestines from each group and compared. Metagenomic, metabolomic, and proteomic analyses were performed on rat feces, serum, and intestines, respectively. Results: Histopathological studies showed that Z14G exposure resulted in dysplasia of gut-associated lymphoid tissue (GALT) compared to ZEN exposure. The elimination of gut microbes in the PGF-Z14G-H group alleviated or eliminated Z14G-induced intestinal toxicity and GALT dysplasia. Metagenomic analysis revealed that Z14G exposure significantly promoted the proliferation of Bifidobacterium and Bacteroides compared to ZEN. Metabolomic analysis showed that Z14G exposure significantly reduced bile acid, while proteomic analysis found that Z14G exposure significantly reduced the expression of C-type lectins compared to ZEN. Conclusions: Our experimental results and previous research suggest that Z14G is hydrolyzed to ZEN by Bifidobacterium and Bacteroides promoting their co-trophic proliferation. This leads to inactivation of lectins by hyperproliferative Bacteroides when ZEN caused intestinal involvement, resulting in abnormal lymphocyte homing and ultimately GALT dysplasia. It is noteworthy that Z14G is a promising model drug to establish rat models of intestinal nodular lymphatic hyperplasia (INLH), which is of great significance for studying the pathogenesis, drug screening and clinical application of INLH

Thiessen polygon analysis and spatial pattern evolution of Neolithic cultural sites (8.0-4.0 ka BP) in Huaibei Plain of Anhui, East China

The Thiessen polygon concept was applied to analyze how Neolithic sites were gathered and the features of agriculture, living environment and transportation in the Huaibei Plain, Anhui Province, East China, in the Shishanzi cultural period (8.0-6.5 ka BP), Dawenkou cultural period (6.5-4.5 ka BP), and Longshan cultural period (4.5-4.0 ka BP). The Thiessen polygon analysis method was effective in determining the settlement center of the region and helped to confer the complex relationships among agriculture, the living environment and transportation during the Neolithic Age. From the Shishanzi cultural period to the Longshan cultural period, a growing number of small polygons showed enhancement in the degree of gathering and cultural communication. In the Dawenkou and Longshan cultural periods, the central part of Huaibei Plain was the settlement center and the distance from the center of each Thiessen polygon to its edges was decreasing. This indicates that the ancients' dependence on agriculture and development conditions increased over the three cultures. The development of Neolithic cultures was also affected by the environmental changes in the Huaibei Plain. From the Shishanzi cultural period to the Longshan cultural period, the climate tended to be dry, and the area of marsh decreased. However, there was little change in temperature, which was beneficial to the ancients' lives and production. Not only did cultural sites and small polygons increase constantly, but groups of sites and settlement centers also appeared in the central part of plain and areas along the main stream of the Huai River. The Neolithic culture developed vigorously, and the abilities of ancients to adapt to environment and transform nature gradually improved. The NW to SE direction of the small polygons' distribution suggests the control of the ancient rivers on transportation in the Huaibei Plain

Sedimentary control on the formation of a multi-superimposed gas system in the development of key layers in the sequence framework

Based on core observations, well logs and test results of siderite-bearing mudstone from the Benxi Formation to the Member 2 of the Shanxi Formations in the Linxing block, northeastern Ordos Basin, a logging identification model for siderite-bearing mudstone (key layer) was established. The porosity characteristics and sealing property were quantitatively evaluated by logging data. Sedimentary control on the formation of multi-superimposed gas-bearing system in the development of key layers in the sequence framework was also discussed. The results showed that the siderite-bearing mudstone has obvious logging response characteristics, e.g., high photoelectric absorption cross-section index (PE), high density (DEN), high amplitude natural gamma ray (GR), low acoustic (AC), low resistivity (M2RX) and low neutron porosity (CNCF). The quantitatively evaluated results of the porosity characteristics and sealing property for the key layer showed that the key layer has the characteristics of low porosity (with an average of 1.20 percent), low permeability (with an average of 2.29 × 10μm), and high breakthrough pressure (with an average of 12.32 MPa) in the study area. This layer acts as an impermeable gas barrier in a multi-superimposed gas system. The results also indicated that the material composition of the multi-superimposed gas-bearing system can be established by the sequence stratigraphic framework. The sedimentary evolution results in a cyclic rhythm of material composition vertically. The spatial distribution of the corresponding transgressive event layer near the maximum flooding surface (MFS) in the sequence framework restricts the spatial distribution of the key layer with high breakthrough pressure and low porosity, which constitutes the gas-bearing system boundary. The siderite-bearing mudstone formed near the MFS in the second-order sequence and constitutes a stable comparison of the first-order gas-bearing system boundary, which has a wide range of regional distribution and stable thickness. The siderite-bearing mudstone formed near the MFS in the third-order sequence is often incompletely preserved due to the late (underwater) diversion channel erosion and cutting. This layer forms the coal-bearing reservoirs, which we termed as a second-order gas-bearing system in adjacent third-order sequences to form a uniform gas-bearing system

Isolation of a T7-Like Lytic <i>Pasteurella</i> Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against <i>Pasteurella multocida</i> Infections

A lytic bacteriophage PHB01 specific for Pasteurella multocida type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family Podoviridae, order Caudovirales, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was able to infect most of the non-toxigenic P. multocida type D strains tested, but not toxigenic type D strains and those belonging to other capsular types. Phage PHB01, the first lytic phage specific for P. multocida type D sequenced thus far, presents a 37,287-bp double-stranded DNA genome with a 223-bp terminal redundancy. The PHB01 genome showed the highest homology with that of PHB02, a lytic phage specific for P. multocida type A. Phylogenetic analysis showed that PHB01 and PHB02 were composed of a genus that was close to the T7-virus genus. In vivo tests using mouse models showed that the administration of PHB01 was safe to the mice and had a good effect on treating the mice infected with different P. multocida type D strains including virulent strain HN05. These findings suggest that PHB01 has a potential use in therapy against infections caused by P. multocida type D