Palmitate acid promotes gastric cancer metastasis via FABP5/SP1/UCA1 pathway

Abstract Background Gastric cancer (GC) has a clear predilection for metastasis toward omentum which is primarily composed of adipose tissue, combine with our previous research that long non-coding RNA Urothelial cancer associated 1 (UCA1) could promote the peritoneal metastasis of GC, we put forward the hypothesis that fatty acids (FAs) might contribute to these phenomena and a connection between FAs and UCA1 might exist. Methods TCGA database was applied to investigate the expression levels of UCA1 in GC tissues and normal gastric tissues and its correlation with GC patients’ survival. Transfection of siRNA was utilized to knockdown cellular levels of FA-binding protein 5 (FABP5), SP1, UCA1. Migration assay and invasion assay were performed to assess the biological effects of palmitate acid (PA), FABP5, SP1 and UCA1 on GC metastasis. The underlying mechanism was investigated via western blot, immunofluorescence (IF), semi-quantitative RT-PCR (sqRT-PCR) and quantitative RT-PCR (qRT-PCR) analysis. Results Here we demonstrated that PA could promote the nuclear transport of FABP5, which then increased the nuclear protein levels of SP1. Consequently, GC cellular expression levels of UCA1 were increased which promoted the metastatic properties of GC. Besides, the cellular levels of UCA1 in GC tumor tissues were significantly higher than that in normal tissues. Its levels in GC tumor tissues also negatively correlated with the prognosis of GC patients using TCGA database. Conclusions Our research revealed the potential tumor-promoting effect of FA transport protein FABP5. We also established a connection between non-coding RNA and FA metabolism, treatment targeted either to patients’ diets or FABP5 might improve the prognosis of GC patients

The Relationship between Impulsivity and Internet Addiction in Chinese College Students: A Moderated Mediation Analysis of Meaning in Life and Self-Esteem

<div><p>Internet addiction (IA) has increasingly been recognized as a serious psychological malady among college students. Impulsivity has been shown to be associated to addictive behaviors, also to IA, and that the purpose of the study is to investigate whether or not there are variables modulating the relation between impulsivity and IA. “Meaning in life” is regarded as a desirable attribute, with positive mental health outcomes. “Self-esteem” is often regarded as an important component of psychological health which has relation to IA. Therefore, we examined meaning in life and self-esteem’s possible effects in this relationship. A total of 1068 Chinese college students ranging in age from 18 to 25 years were recruited for this cross-sectional survey study. Correlations and multivariate regressions were used to calculate the possible mediation and moderation relationship among the variables of meaning in life, self-esteem, impulsivity, and IA. In the analyses that we conducted, IA was shown to be prevalent among Chinese university students. The relationship between impulsivity and IA was partially mediated by meaning in life, and the relationship between meaning in life and IA was moderated by self-esteem. Our findings demonstrate that meaning in life and self-esteem can be useful buffers to IA for highly impulsive individuals. Further randomized trials to confirm these results are needed.</p></div

Standardized regression coefficients for paths within the mediation model

<p>. Explained variance: β, standardized regression coefficient.</p

Means, standard deviations, intercorrelations, and internal consistency between study variables.

<p>Note: N = 1068;</p><p>** <i>p</i> < 0.01.</p><p>Means, standard deviations, intercorrelations, and internal consistency between study variables.</p

Summary of hierarchical regression analysis for meaning in life in mediating the relationship between impulsivity and internet addiction.

<p>Note:</p><p>*** <i>p</i> < 0.001</p><p>Explained variance: step 1, <i>R</i>^<i>2</i> = .606; step 2, <i>R</i>^<i>2</i> = .561; step 3, <i>R</i>^<i>2</i> = .763; <i>B</i>, unstandardized regression coefficient; <i>SE</i>, standard error of <i>B</i>.</p><p>Summary of hierarchical regression analysis for meaning in life in mediating the relationship between impulsivity and internet addiction.</p

The buffering effect of self-esteem on the relationship between meaning in life and internet addiction.

<p>Note:</p><p>*** <i>p</i> < 0.001,</p><p>* <i>p</i> ≤ 0.05.</p><p>The buffering effect of self-esteem on the relationship between meaning in life and internet addiction.</p