A Molecular Dynamics Analysis of the Thickness and Adhesion Characteristics of the Quasi-Liquid Layer at the Asphalt–Ice Interface

The quasi-liquid layer (QLL), a microstructure located between ice and an adhering substrate, is critical in generating capillary pressure, which in turn influences ice adhesion behavior. This study employed molecular dynamics (MD) methods to obtain QLL thickness and utilized these measurements to estimate the adhesive strength between ice and asphalt. The research involved constructing an ice–QLL–asphalt MD model, encompassing four asphalt types and five temperature ranges from 250 K to 270 K. The QLL thickness was determined for various asphalts and temperatures using the tetrahedral order parameter gradient. Additionally, capillary pressure was calculated based on the QLL thickness and other geometric parameters obtained from the MD analysis. These findings were then compared with ice adhesion strength data acquired from pull-off tests. The results indicate that QLL thickness varies with different asphalt types and increases with temperature. At a constant temperature, the QLL thickness decreases in the order of the basal plane, primary prism plane, and secondary prism plane. Furthermore, the adhesion strength of the QLL diminishes as the temperature rises, attributed to the disruption of hydrogen bonds at lower temperatures. The greater the polarity of the asphalt’s interface molecules, the stronger the adhesion strength and binding free energy. The MD simulations of the asphalt–ice interface offer insights into the atomic-scale adhesive properties of this interface, contributing to the enhancement in QLL property prediction and calibration at larger scales

Improving border irrigation performance with predesigned varied-discharge.

Insufficient water resources restrict wheat production in the North China Plain, so it is urgent and essential to improve the border irrigation performance and water use efficiency. This study developed a predesigned varied-discharge irrigation scheme in the closed-ended border. Field treatments, including continuous-discharge (CD), increased-discharge (ID) and decreased-discharge (DD) border irrigation tests, were conducted to evaluate the irrigation performance of the proposed varied-discharge scheme. The DD border irrigation treatment had great application efficiency (AE), distribution uniformity (DU) and requirement efficiency (RE), and its comprehensive evaluation indicator (Y) was also significantly higher than other treatments. DD treatment achieved the average AE, DU, RE and Y values of 91.4%, 95.5%, 99.5% and 95.4%, respectively. Furthermore, the hydraulic simulation model WinSRFR was used to optimize the scheme of predesigned varied-discharge border irrigation, and sensitivity analyses of infiltration parameters, roughness coefficient, slope and inflow rate were carried out. The results indicate that the predesigned varied-discharge border irrigation scheme can improve the irrigation performance, and the DD border irrigation scheme has more satisfactory robustness than that of the ID border irrigation scheme

Enhanced N2O Emissions from Winter Wheat Field Induced by Winter Irrigation in the North China Plain

Winter irrigation is important for wheat in meeting crop water requirements in spring, but it alters soil moisture dynamics and affects soil N2O production and emission. To assess the effects of winter irrigation on soil N2O emissions in a winter wheat field, an in situ experiment was conducted from 1 October 2019 to 1 March 2020 with one control treatment (CK) and five levels of winter irrigation quantities (irrigated to 60%, 70%, 80%, 90%, 100% of the soil water holding capacity, namely WHC60–WHC100, respectively). The results showed that winter irrigation had an impact on soil N2O emission. The emission peaks were not investigated immediately after winter irrigation, but at two days after, which were increased by 4.3–17.0 μg·m−2·h−1 under WHC60–100 treatments compared to the CK. The cumulative N2O emissions after winter irrigation from WHC60–100 were 1.1–3.9 times higher than that of CK, indicating that the cumulative N2O emission has an increase trend with the increase of soil water content regulated by irrigations. Pearson correlation analysis showed that the correlation between soil N2O flux and soil temperature were moderate with correlation coefficients of about 0.65. While the correlation between soil N2O flux and soil water content was poor during the investigate winter season with correlation coefficients ranging between 0.08 and 0.25. Future studies should focus on the general N2O emission responses to winter irrigation and environmental factors with the support of experiment data from several winter seasons

Enhanced N₂O Emissions from Winter Wheat Field Induced by Winter Irrigation in the North China Plain

Winter irrigation is important for wheat in meeting crop water requirements in spring, but it alters soil moisture dynamics and affects soil N2O production and emission. To assess the effects of winter irrigation on soil N2O emissions in a winter wheat field, an in situ experiment was conducted from 1 October 2019 to 1 March 2020 with one control treatment (CK) and five levels of winter irrigation quantities (irrigated to 60%, 70%, 80%, 90%, 100% of the soil water holding capacity, namely WHC60–WHC100, respectively). The results showed that winter irrigation had an impact on soil N2O emission. The emission peaks were not investigated immediately after winter irrigation, but at two days after, which were increased by 4.3–17.0 μg·m−2·h−1 under WHC60–100 treatments compared to the CK. The cumulative N2O emissions after winter irrigation from WHC60–100 were 1.1–3.9 times higher than that of CK, indicating that the cumulative N2O emission has an increase trend with the increase of soil water content regulated by irrigations. Pearson correlation analysis showed that the correlation between soil N2O flux and soil temperature were moderate with correlation coefficients of about 0.65. While the correlation between soil N2O flux and soil water content was poor during the investigate winter season with correlation coefficients ranging between 0.08 and 0.25. Future studies should focus on the general N2O emission responses to winter irrigation and environmental factors with the support of experiment data from several winter seasons

WTAP-mediated m6A modification modulates bone marrow mesenchymal stem cells differentiation potential and osteoporosis

Abstract An imbalance in the differentiation potential of bone marrow mesenchymal stem cells (BMSCs) is an important pathogenic mechanism underlying osteoporosis (OP). N6-methyladenosine (m6A) is the most common post-transcriptional modification in eukaryotic cells. The role of the Wilms’ tumor 1-associated protein (WTAP), a member of the m6A functional protein family, in regulating BMSCs differentiation remains unknown. We used patient-derived and mouse model-derived samples, qRT-PCR, western blot assays, ALP activity assay, ALP, and Alizarin Red staining to determine the changes in mRNA and protein levels of genes and proteins associated with BMSCs differentiation. Histological analysis and micro-CT were used to evaluate developmental changes in the bone. The results determined that WTAP promoted osteogenic differentiation and inhibited adipogenic differentiation of BMSCs. We used co-immunoprecipitation (co-IP), RNA immunoprecipitation (RIP), methylated RNA immunoprecipitation (MeRIP), RNA pulldown, and dual-luciferase assay to explore the direct mechanism. Mechanistically, the expression of WTAP increased during osteogenic differentiation and significantly promoted pri-miR-181a and pri-miR-181c methylation, which was recognized by YTHDC1, and increased the maturation to miR-181a and miR-181c. MiR-181a and miR-181c inhibited the mRNA expression of SFRP1, promoting the osteogenic differentiation of BMSCs. Our results demonstrated that the WTAP/YTHDC1/miR-181a and miR-181c/SFRP1 axis regulated the differentiation fate of BMSCs, suggesting that it might be a potential therapeutic target for osteoporosis

Increased Cerebral Level of P2X7R in a Tauopathy Mouse Model by PET Using [18F]GSK1482160.

Neuroinflammation plays an important role in Alzheimer's disease and primary tauopathies. The aim of the current study was to map [18F]GSK1482160 for imaging of purinergic P2X7R in Alzheimer's disease and primary tauopathy mouse models. Small animal PET was performed using [18F]GSK1482160 in widely used mouse models of Alzheimer's disease (APP/PS1, 5×FAD, and 3×Tg), 4-repeat tauopathy (rTg4510) mice, and age-matched wild-type mice. Increased uptake of [18F]GSK1482160 was observed in the brains of 7-month-old rTg4510 mice compared to wild-type mice and compared to 3-month-old rTg4510 mice. A positive correlation between hippocampal tau [18F]APN-1607 and [18F]GSK1482160 uptake was found in rTg4510 mice. No significant differences in the uptake of [18F]GSK1482160 was observed for APP/PS1 mice, 5×FAD mice, or 3×Tg mice. Immunofluorescence staining further indicated the distribution of P2X7Rs in the brains of 7-month-old rTg4510 mice with accumulation of tau inclusion. These findings provide in vivo imaging evidence for an increased level of P2X7R in the brains of tauopathy mice

Arginine methylation of PPP1CA by CARM1 regulates glucose metabolism and affects osteogenic differentiation and osteoclastic differentiation

Abstract Background The imbalance between osteoblasts and osteoclasts may lead to osteoporosis. Osteoblasts and osteoclasts have different energy requirements, with aerobic glycolysis being the prominent metabolic feature of osteoblasts, while osteoclast differentiation and fusion are driven by oxidative phosphorylation. Methods By polymerase chain reaction as well as Western blotting, we assayed coactivator‐associated arginine methyltransferase 1 (CARM1) expression in bone tissue, the mouse precranial osteoblast cell line MC3T3‐E1 and the mouse monocyte macrophage leukaemia cell line RAW264.7, and expression of related genes during osteogenic differentiation and osteoclast differentiation. Using gene overexpression (lentivirus) and loss‐of‐function approach (CRISPR/Cas9‐mediated knockout) in vitro, we examined whether CARM1 regulates osteogenic differentiation and osteoblast differentiation by metabolic regulation. Transcriptomic assays and metabolomic assays were used to find the mechanism of action of CARM1. Furthermore, in vitro methylation assays were applied to clarify the arginine methylation site of PPP1CA by CARM1. Results We discovered that CARM1 reprogrammed glucose metabolism in osteoblasts and osteoclasts from oxidative phosphorylation to aerobic glycolysis, thereby promoting osteogenic differentiation and inhibiting osteoclastic differentiation. In vivo experiments revealed that CARM1 significantly decreased bone loss in osteoporosis model mice. Mechanistically, CARM1 methylated R23 of PPP1CA, affected the dephosphorylation of AKT‐T450 and AMPK‐T172, and increased the activities of phosphofructokinase‐1 and pructose‐2,6‐biphosphatase3, causing an up‐regulation of glycolytic flux. At the same time, as a transcriptional coactivator, CARM1 regulated the expression of pyruvate dehydrogenase kinase 3, which resulted in the inhibition of pyruvate dehydrogenase activity and inhibition of the tricarboxylic acid cycle, leading to a subsequent decrease in the flux of oxidative phosphorylation. Conclusions These findings reveal for the first time the mechanism by which CARM1 affects both osteogenesis and osteoclast differentiation through metabolic regulation, which may represent a new feasible treatment strategy for osteoporosis