Effect of compounds on the purification and antibody preparation of the extracellular domain fragment of the receptor CD163

<p>Abstract</p> <p>Background</p> <p>Porcine reproductive and respiratory syndrome virus (PRRSV) has been acknowledged as one of the most important agents affecting swine. The scavenger receptor CD163 is one of the important entry mediators for PRRSV.</p> <p>Results</p> <p>The tD4 and tD5 CD163 genes were amplified, and the PCR products were cloned into pET-28a(+) (designated pET-28a-tD4 and pET-28a-tD5, respectively). The plasmids pET-28a-tD4 and pET-28a-tD5 were then transformed into the <it>E. coli </it>BL21 (DE3) strain and expressed by adding 1 mmol/L of isopropyl-beta-D-thiogalactopyranoside. The proteins were highly expressed in the supernatant from the tD4- and tD5-producing cells that were incubated with a binding buffer containing the following compounds: β-mercaptoethanol, urea, Tween 20, glycerol, and SDS, while they were rarely expressed in the supernatant from the tD4- and tD5-producing cells that were incubated with binding buffer without the compounds. The tD4 and tD5 proteins were purified, and BALB/c mice were immunized with the purified proteins. Western blotting analysis showed that the tD4 and tD5 proteins were capable of reacting with tD5 antibodies; the titer of both the tD4 and tD5 antiserums was 1:160 against the tD5 protein, as shown by ELISA.</p> <p>Conclusions</p> <p>These studies provide a new way for the purification of proteins expressed in inclusion bodies and the preparation of the corresponding antibodies.</p

Genetic characterization of H1N2 influenza a virus isolated from sick pigs in Southern China in 2010

In China H3N2 and H1N1 swine influenza viruses have been circulating for many years. In January 2010, before swine were infected with foot and mouth disease in Guangdong, some pigs have shown flu-like symptoms: cough, sneeze, runny nose and fever. We collected the nasopharyngeal swab of all sick pigs as much as possible. One subtype H1N2 influenza viruses were isolated from the pig population. The complete genome of one isolate, designated A/swine/Guangdong/1/2010(H1N2), was sequenced and compared with sequences available in GenBank. The nucleotide sequences of all eight viral RNA segments were determined, and then phylogenetic analysis was performed using the neighbor-joining method. HA, NP, M and NS were shown to be closely to swine origin. PB2 and PA were close to avian origin, but NA and PB1were close to human origin. It is a result of a multiple reassortment event. In conclusion, our finding provides further evidence about the interspecies transmission of avian influenza viruses to pigs and emphasizes the importance of reinforcing swine influenza virus (SIV) surveillance, especially before the emergence of highly pathogenic FMDs in pigs in Guangdong

Search for hidden-charm tetraquark with strangeness in e+e−→K+Ds∗−D∗0+c.c.e^{+}e^{-}\rightarrow K^+ D_{s}^{*-} D^{*0}+c.c.

We report a search for a heavier partner of the recently observed $Z_{cs}(3985)^{-}$ state, denoted as $Z_{cs}^{\prime -}$, in the process $e^{+} e^{-}\rightarrow K^{+}D_{s}^{*-}D^{* 0}+c.c.$, based on $e^+e^-$ collision data collected at the center-of-mass energies of $\sqrt{s}=4.661$, 4.682 and 4.699 GeV with the BESIII detector. The $Z_{cs}^{\prime -}$ is of interest as it is expected to be a candidate for a hidden-charm and open-strange tetraquark. A partial-reconstruction technique is used to isolate $K^+$ recoil-mass spectra, which are probed for a potential contribution from $Z_{cs}^{\prime -}\to D_{s}^{*-}D^{* 0}$ ($c.c.$). We find an excess of $Z_{cs}^{\prime -}\rightarrow D_{s}^{*-}D^{*0}$ ($c.c.$) candidates with a significance of $2.9\sigma$, after considering systematic uncertainties, at a mass of $(4123.5 \pm 0.7_{\mathrm{stat.}} \pm 1.1_{\mathrm{syst.}}) \mathrm{MeV}/c^{2}$. As the data set is limited in size, the upper limits are evaluated at the 90% confidence level on the product of the Born cross section and the branching fraction of $Z_{cs}^{\prime-}\rightarrow D_{s}^{*-}D^{* 0}$, $\sigma^{\rm Born}\cdot\mathcal{B}$ at the three energy points, under different assumptions of the $Z_{cs}^{\prime -}$ mass from 4.120 to 4.140 MeV and of the width from 10 to 50 MeV. Under various mass and width assumptions, the upper limits of $\sigma^{\rm Born}\cdot\mathcal{B}$ are found to lie in the range of $2\sim6$, $3\sim7$ and $3\sim6$ pb at $\sqrt{s}=4.661$, 4.682 and 4.699 GeV, respectively. The larger data samples that will be collected in the coming years will allow a clearer picture to emerge concerning the existence and nature of the $Z_{cs}^{\prime -}$ state.Comment: 17 pages, 7 figure

Study of η(1405)/η(1475)\eta(1405)/\eta(1475) in J/ψ→γKS0KS0π0J/\psi\to\gamma K^{0}_{S} K^{0}_{S}\pi^{0} decay

Using a sample of $(10.09\pm0.04)\times10^{9}$ $J/\psi$ decays collected with the BESIII detector, partial wave analyses of the decay $J/\psi\to\gamma K^{0}_{S} K^{0}_{S}\pi^{0}$ are performed within the $K^{0}_{S} K^{0}_{S}\pi^{0}$ invariant mass region below 1.6 ${\textrm{GeV}/c^2}$. The covariant tensor amplitude method is used in both mass independent and mass dependent approaches. Both analysis approaches exhibit dominant pseudoscalar and axial vector components, and show good consistency for the other individual components. Furthermore, the mass dependent analysis reveals that the $K^{0}_{S} K^{0}_{S}\pi^{0}$ invariant mass spectrum for the pseudoscalar component can be well described with two isoscalar resonant states, ${\it i.e.}$, the $\eta(1405)$ with a mass of $1391.7\pm0.7_{-0.3}^{+11.3}$ $\textrm{MeV}/c^2$ and a width of $60.8\pm1.2_{-12.0}^{+5.5}$ $\textrm{MeV}$, and the $\eta(1475)$ with a mass of $1507.6\pm1.6_{-32.2}^{+15.5}$ $\textrm{MeV}/c^2$ and a width of $115.8\pm2.4_{-10.9}^{+14.8}$ $\textrm{MeV}$. The first and second uncertainties are statistical and systematic, respectively. Alternate models for the pseudoscalar component are also tested, but the description of the $K^{0}_{S} K^{0}_{S}\pi^{0}$ invariant mass spectrum deteriorates significantly.Comment: 20 pages, 6 figures, 6 table

Production of doubly-charged Δ\Delta baryon in e+e−e^{+}e^{-} annihilation at energies from 2.3094 to 2.6464 GeV

The processes $e^{+}e^{-} \to \Delta^{++}\bar{\Delta}^{--}$ and $e^{+}e^{-}\to \Delta^{++} \bar{p} \pi^{-} + c.c.$ are studied for the first time with $179~{\rm pb}^{-1}$ of $e^{+}e^{-}$ annihilation data collected with the BESIII detector at center-of-mass energies from $2.3094$ GeV to $2.6464$ GeV. No significant signal for the $e^{+}e^{-}\to \Delta^{++}\bar{\Delta}^{--}$ process is observed and the upper limit of the Born cross section is estimated at each energy point. For the process $e^{+}e^{-} \to \Delta^{++} \bar{p} \pi^{-} + c.c.$, a significant signal is observed at center-of-mass energies near 2.6454 GeV and the corresponding Born cross section is reported.Comment: 10 pages, 4 figure

Measurement of the C ⁣PC\!P-even fraction of D0→K+K−π+π−D^0\to K^+K^-\pi^+\pi^-

A determination of the $C\!P$-even fraction $F_+$ in the decay $D^0 \to K^+K^-\pi^+\pi^-$ is presented. Using $2.93$ fb$^{-1}$ of $e^+e^-\to\psi(3770)\to D\bar{D}$ data collected by the BESIII detector, one charm meson is reconstructed in the signal mode and the other in a $C\!P$ eigenstate or the decay $D\to K_{S, L}^0\pi^+\pi^-$. Analysis of the relative rates of these double-tagged events yields the result $F_+ = 0.730 \pm 0.037 \pm 0.021$, where the first uncertainty is statistical and the second is systematic. This is the first model-independent measurement of $F_+$ in $D^0 \to K^+K^-\pi^+\pi^-$ decays.Comment: 13 pages, 5 figure

Observation of the decay ψ(3686)→Σ−Σˉ+\psi(3686) \to \Sigma^-\bar\Sigma^+ and measurement of its angular distribution

Using $(448.1\pm2.9)\times10^6$ $\psi(3686)$ events collected with the BESIII detector at the BEPCII collider, the decay $\psi(3686)\to\Sigma^-\bar\Sigma^+$ is observed for the first time with a branching fraction of $(2.82\pm0.04_{\rm stat.}\pm0.08_{\rm syst.})\times10^{-4}$, and the angular parameter $\alpha_{ \Sigma^-}$ is measured to be $0.96\pm0.09_{\rm stat.} \pm 0.03_{\rm syst.}$

Search for an axion-like particle in J/ψJ/\psi radiative decays

We search for an axion-like particle (ALP) $a$ through the process $\psi(3686)\rightarrow\pi^+\pi^-J/\psi$, $J/\psi\rightarrow\gamma a$, $a\rightarrow\gamma\gamma$ in a data sample with $(2708.1\pm14.5)\times10^6$ $\psi(3686)$ events collected by the BESIII detector. No significant ALP signal is observed over the expected background, and the upper limits on the branching fraction of the decay $J/\psi\rightarrow\gamma a$ and the ALP-photon coupling constant $g_{a\gamma\gamma}$ are set at the 95\% confidence level in the mass range of 0.165\leq m_a\leq2.84\,\mbox{GeV}/c^2. The limits on $\mathcal{B}(J/\psi\rightarrow\gamma a)$ range from $8.3\times10^{-8}$ to $1.8\times10^{-6}$ over the search region, and the constraints on the ALP-photon coupling are the most stringent to date for 0.165\leq m_a\leq1.468\,\mbox{GeV}/c^2.Comment: 10 pages, 5 figure

Observation of the J/ψJ/\psi and ψ(3686)\psi(3686) decays into ηΣ+Σˉ−\eta\Sigma^{+}\bar{\Sigma}^{-}

The decays $J/\psi\to\eta\Sigma^{+}\bar{\Sigma}{}^-$ and $\psi(3686)\to\eta\Sigma^{+}\bar{\Sigma}{}^-$ are observed for the first time, using $(10087 \pm 44)\times 10^{6}$ $J/\psi$ and $(448.1 \pm 2.9)\times 10^{6}$ $\psi(3686)$ events collected with the BESIII detector at the BEPCII collider. We determine the branching fractions of these two decays to be ${\cal B}(J/\psi\to\eta\Sigma^{+}\bar{\Sigma}{}^-)=(6.34 \pm 0.21 \pm 0.37)\times 10^{-5}$ and ${\cal B}(\psi(3686)\to\eta\Sigma^{+}\bar{\Sigma}{}^-)=(9.59 \pm 2.37 \pm 0.61)\times 10^{-6}$, where the first uncertainties are statistical and the second are systematic. The ratio of these two branching fractions is determined to be $\frac{{\cal B}(\psi(3686)\to\eta\Sigma^{+}\bar{\Sigma}{}^-)}{{\cal B}(J/\psi\to\eta\Sigma^{+}\bar{\Sigma}{}^-)}=(15.1 \pm 3.8)\%$, which is in agreement with the "12\% rule."Comment: 9 pages and 10 figure

Search for the weak decay $\psi(3686) \to \Lambda_c^{+} \bar{\Sigma}^- +c.c

Using $(448.1 \pm 2.9) \times 10^{6}$ $\psi(3686)$ events collected with the BESIII detector, we perform the first search for the weak baryonic decay $\psi(3686) \to \Lambda_c^{+} \bar{\Sigma}^- +c.c.$. The analysis procedure is optimized using a blinded method. No significant signal is observed, and the upper limit on the branching fraction ($\mathcal B$) of $\psi(3686) \to \Lambda_c^{+} \bar{\Sigma}^- +c.c.$ is set to be $1.4\times 10^{-5}$ at the 90\% confidence level.Comment: 9 pages, 4 figure