Additional file 3 of THZ1 targeting CDK7 suppresses c-KIT transcriptional activity in gastrointestinal stromal tumours

Additional file 2. The different expression gene set of GIST-T1 cells after THZ1 treatment by RNA-sequencing analysis

Additional file 2 of THZ1 targeting CDK7 suppresses c-KIT transcriptional activity in gastrointestinal stromal tumours

Additional file 1. The siRNA sequences and qRT-PCR primer sequences used in this research.

Intratumor percentage of double-positive cells compared with IL-17⁺ cells.

<p>Cell numbers were assessed in ten independent fields at 400× magnification. Average cell numbers (third column) and ratios to the total amount of IL-17⁺ cells (fourth column) for CD3⁺IL-17⁺ (n = 20), CD4⁺IL-17⁺ (n = 20), CD68⁺IL-17⁺ (n = 20), MCT⁺IL-17⁺ (n = 20) cells were shown.</p><p>Intratumor percentage of double-positive cells compared with IL-17⁺ cells.</p

Gastric cancer tissue samples (n = 20) were stained for IL-17 (red and green) and indicated markers.

<p>Sections were counterstained with DAPI. Markers of CD3 or CD4 (green) and B cell marker CD20 (green), nature killer (NK) marker CD56 (green) and macrophage marker CD68 (orange), as well as mast cell tryptase (MCT) (orange) are shown at 400× magnifications.</p

Intratumor accumulation of IL-17⁺ cells and MCT⁺IL-17⁺ cells predicts poor survival in gastric cancer patients.

<p>(A) The overall survival for all enrolled 112 patients (B) High intratumor IL-17⁺ cells infiltration conferred a significant high risk of death. (C) Patients with high MCT⁺IL-17⁺ cell intratumor had significant poorer survival than patients with low MCT⁺IL-17⁺ cell.</p

Correlation between tumor-infiltrated parameters with MCT⁺IL-17⁺ cells.

<p>(A) CD34⁺ microvessel density. (B) CD66b⁺ neutrophils. (C) CD68⁺ macrophages. (D) FoxP3⁺ lymphocytes. P values were determined by Pearson correlation coefficient for CD34⁺ microvessels and FoxP3⁺ lymphocytes, while Spearman correlation coefficient was used for CD66b⁺ neutrophils and CD68⁺ macrophages analysis.</p

Colocalization between IL-17R (green) and CD34⁺ vascular endothelial cells (orange).

<p>Colocalization between IL-17R (green) and CD34⁺ vascular endothelial cells (orange).</p

Immunostaining for IL-17 in gastric cancer show different cell morphology.

<p>Squares indicate different examples (brown, shown at 400× magnification).</p

The prevalence of MCT⁺ cells (orange) and IL-17⁺ cells (green) in 112 paired gastric cancer tissue samples.

<p>A. Mast cells highly express IL-17 in gastric cancer (merged in yellow, right panel). B. MCT⁺ cells and IL-17⁺ cells, as well as MCT⁺IL-17⁺ cells in intratumor tissues were significant higher than those of corresponding normal tissues.</p

Univariate and Multivariate analysis of factors associated with overall survival in 112 gastric cancer patients.

<p>HR hazard ratio; CI confidence interval; NA not applicable; NS not significant.</p>a<p>7^th Edition of American Joint of Committee On Cancer.</p>b<p>Univariate analysis were performed by the Kaplan-Meier analysis model and log-rank test.</p>c<p>Multivariate analysis were performed by Cox proportional hazards models with the backward likelihood stepwise procedures.</p><p>Univariate and Multivariate analysis of factors associated with overall survival in 112 gastric cancer patients.</p