The densitometry analysis of protein expression.

<p>The vertical axis represented the densitometry ratio of protein HIF and β-actin, and the horizontal axis represented the different samples of Western blot assay.</p

Expression of p<i>AS</i>HIF protein in polyps and medusa.

<p>The <i>AS</i>HIF recombinant fragment protein was used as the positive control, and the actin protein was used as the standard to determine the quantity of the total protein. M represented protein marker, N represented reference group (normoxia condition), H represented hypoxic group and P was positive control.</p

Alignment of HIF-1α deduced amino acid sequences with <i>T. adhaerens</i>, <i>H. magnipapillata</i>, <i>N. vectensis</i>, <i>A</i> sp.1, <i>L. vannamei</i>, <i>S. purpuratus</i> and <i>H. sapiens</i> homologues.

<p>(A) The functional domains that are included as solid boxes contain bHLH and PAS domain. (B) The N-ODD domains. (C) The C-ODD domains. (D) The C-TAD domains.</p

The genomic structure, length and organization of <i>HIF-1α</i> from <i>T. adhaerens</i>, <i>H. magnipapillata</i>, <i>N. vectensis</i>, <i>Aurelia</i> sp.1, <i>S. purpuratus</i> and <i>H. sapiens</i>.

<p>Boxes represented the sequences of exons of the gene and thin lines indicated the sequences of introns. Values above the lines and the boxes indicated the sizes of the introns and exons, respectively. “E1 to E15” represented “Exon1 to Exon15”. The exons included bHLH domain (highlighted in green), PAS domain (highlighted in red), N-ODD domain (highlighted in yellow), C-ODD domain (highlighted in blue), and C-TAD domain (highlighted in pink).</p

The protein domain structure alignment of HIF-1α with <i>T. adhaerens</i>, <i>H. magnipapillata</i>, <i>N. vectensis</i>, <i>Aurelia</i> sp.1, <i>S. purpuratus</i> and <i>H. sapiens</i>.

<p>The protein domain structure alignment of HIF-1α with <i>T. adhaerens</i>, <i>H. magnipapillata</i>, <i>N. vectensis</i>, <i>Aurelia</i> sp.1, <i>S. purpuratus</i> and <i>H. sapiens</i>.</p

The accession numbers in GenBank for <i>HIF</i> nucleic acid sequences in alignment.

<p><b>*</b>The sequence came from DOE Joint Genome Institute.</p

Nucleotide and predicted amino acid sequence of HIF-1α in <i>Aurelia</i> sp.1.

<p>The asterisk indicated the stop codon, and the shadow portion was used as the recombinant fragment to generate the polyclonal antibody.</p