4 research outputs found
Additional file 4: of Evaluation of skeletal muscle microvascular perfusion of lower extremities by cardiovascular magnetic resonance arterial spin labeling, blood oxygenation level-dependent, and intravoxel incoherent motion techniques
Table S1. Spearman rank correlation coefficients for ASL, BOLD, and IVIM imaging parameters. (DOCX 16 kb
Additional file 2: of Evaluation of skeletal muscle microvascular perfusion of lower extremities by cardiovascular magnetic resonance arterial spin labeling, blood oxygenation level-dependent, and intravoxel incoherent motion techniques
Figure S2. Graphs depicting serial measurements of imaging parameters from ASL, BOLD, and IVIM for the anterior, lateral, soleus, and gastrocnemius muscle groups in the control side in healthy young subjects. (PPTX 727 kb
Additional file 3: of Evaluation of skeletal muscle microvascular perfusion of lower extremities by cardiovascular magnetic resonance arterial spin labeling, blood oxygenation level-dependent, and intravoxel incoherent motion techniques
Figure S3. Graphs depicting serial measurements of imaging parameters from ASL, BOLD, and IVIM for the anterior, lateral, soleus, and gastrocnemius muscle groups in the control side in healthy old subjects. (PPTX 654 kb
Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures
The
DNA origami technology holds great promise for the assembly
of nanoscopic technological devices and studies of biochemical reactions
at the single-molecule level. For these, it is essential to establish
well controlled attachment of functional materials to predefined sites
on the DNA origami nanostructures for reliable measurements and versatile
applications. However, the two-sided nature of the origami scaffold
has shown limitations in this regard. We hypothesized that holes of
the commonly used two-dimensional DNA origami designs are large enough
for the passage of single-stranded (ss)-DNA. Sufficiently long ssDNA
initially located on one side of the origami should thus be able to
“thread” to the other side through the holes in the
origami sheet. By using an origami sheet attached with patterned biotinylated
ssDNA spacers and monitoring streptavidin binding with atomic force
microscopic (AFM) imaging, we provide unambiguous evidence that the
biotin ligands positioned on one side have indeed threaded through
to the other side. Our finding reveals a previously overlooked critical
design feature that should provide new interpretations to previous
experiments and new opportunities for the construction of origami
structures with new functional capabilities